Braking and Body Angles Control of an Insect-Computer Hybrid Robot by Electrical Stimulation of Beetle Flight Muscle in Free Flight

While engineers put lots of effort, resources, and time in building insect scale micro aerial vehicles (MAVs) that fly like insects, insects themselves are the real masters of flight. What if we would use living insect as platform for MAV instead? Here, we reported a flight control via electrical stimulation of a flight muscle of an insect-computer hybrid robot, which is the interface of a mountable wireless backpack controller and a living beetle. The beetle uses indirect flight muscles to drive wing flapping and three major direct flight muscles (basalar, subalar and third axilliary (3Ax) muscles) to control the kinematics of the wings for flight maneuver. While turning control was already achieved by stimulating basalar and 3Ax muscles, electrical stimulation of subalar muscles resulted in braking and elevation control in flight. We also demonstrated around 20 degrees of contralateral yaw and roll by stimulating individual subalar muscle. Stimulating both subalar muscles lead to an increase of 20 degrees in pitch and decelerate the flight by 1.5 m/s2 as well as an induce an elevation of 2 m/s2.Comment: 9 pages, 7 figures, supplemental video: https://youtu.be/P9dxsSf14LY . Cyborg and Bionic Systems 202

Towards understanding of climbing, tip-over prevention and self-righting behaviors in Hexapoda

Die vorliegende Dissertation mit dem Titel “Towards understanding of climbing, tip-over prevention and self-righting behaviors in Hexapoda” untersucht in drei Studien exemplarisch, wie (i) Wüstenameisen ihre Beine einsetzen um An- und Abstiege zu überwinden, wie (ii) Wüsten- und Waldameisen ein Umkippen an steilen Anstiegen vermeiden, und wie sich (iii) Madagaskar-Fauchschaben, Amerikanische Großschaben und Blaberus discoidalis Audinet-Servill, 1839 aus Rückenlagen drehen und aufrichten. Neuartige biomechanischen Beschreibungen umfassen unter anderem: Impuls- und Kraftwirkungen einzelner Ameisenbeine auf den Untergrund beim Bergauf- und Bergabklettern, Kippmomente bei kletternden Ameisen, Energiegebirge-Modelle (energy landscapes) zur Quantifizierung der Körperform für die funktionelle Beschreibung des Umdrehens aus der Rückenlage

Towards Agility: Definition, Benchmark and Design Considerations for Small, Quadrupedal Robots

Agile quadrupedal locomotion in animals and robots is yet to be fully understood, quantified or achieved. An intuitive notion of agility exists, but neither a concise definition nor a common benchmark can be found. Further, it is unclear, what minimal level of mechatronic complexity is needed for this particular aspect of locomotion. In this thesis we address and partially answer two primary questions: (Q1) What is agile legged locomotion (agility) and how can wemeasure it? (Q2) How can wemake agile legged locomotion with a robot a reality? To answer our first question, we define agility for robot and animal alike, building a common ground for this particular component of locomotion and introduce quantitative measures to enhance robot evaluation and comparison. The definition is based on and inspired by features of agility observed in nature, sports, and suggested in robotics related publications. Using the results of this observational and literature review, we build a novel and extendable benchmark of thirteen different tasks that implement our vision of quantitatively classifying agility. All scores are calculated from simple measures, such as time, distance, angles and characteristic geometric values for robot scaling. We normalize all unit-less scores to reach comparability between different systems. An initial implementation with available robots and real agility-dogs as baseline finalize our effort of answering the first question. Bio-inspired designs introducing and benefiting from morphological aspects present in nature allowed the generation of fast, robust and energy efficient locomotion. We use engineering tools and interdisciplinary knowledge transferred from biology to build low-cost robots able to achieve a certain level of agility and as a result of this addressing our second question. This iterative process led to a series of robots from Lynx over Cheetah-Cub-S, Cheetah-Cub-AL, and Oncilla to Serval, a compliant robot with actuated spine, high range of motion in all joints. Serval presents a high level of mobility at medium speeds. With many successfully implemented skills, using a basic kinematics-duplication from dogs (copying the foot-trajectories of real animals and replaying themotion on the robot using a mathematical interpretation), we found strengths to emphasize, weaknesses to correct and made Serval ready for future attempts to achieve even more agile locomotion. We calculated Servalâs agility scores with the result of it performing better than any of its predecessors. Our small, safe and low-cost robot is able to execute up to 6 agility tasks out of 13 with the potential to reachmore after extended development. Concluding, we like to mention that Serval is able to cope with step-downs, smooth, bumpy terrain and falling orthogonally to the ground

Insect-computer hybrid robot achieves a walking gait rarely seen in nature by replacing the anisotropic natural leg spines with isotropic artificial leg spines

This paper demonstrates that our developed beetle–computer hybrid legged robot achieves backward walk, which is impossible by intact beetles themselves in nature. Judging from the curvature of the natural leg spine, we hypothesized that the natural spine has an anisotropic function: the natural spine would provide foot traction only in forward walk but not in backward. The hypothesis was verified as beetles hardly walk backward due to often slips. We then designed an artificial leg spine, which isotropically functions in walk: the foot traction was increased and slipless walk was achieved in both backward and forward walk with the artificial spines being implanted into the beetle legs. For these investigations, a wireless communication device, or “backpack,” was mounted and wired to a live beetle for electrically stimulating leg muscles to remotely modulate leg motions and to perform the forward and backward walk on demand. Overall, the beetle hybrid robot revealed the anisotropic function of the natural leg spine and also achieved the backward walk, which the intact beetle cannot intrinsically perform.Ministry of Education (MOE)Nanyang Technological UniversityThe work was supported in part by the Nanyang Assistant Professorship (NAP, M4080740) and in part by the Singapore Ministry of Education under Grant MOE2013-T2-2-049 and Grant 2016-T1-002-156

Psr1p interacts with SUN/sad1p and EB1/mal3p to establish the bipolar spindle

Regular Abstracts - Sunday Poster Presentations: no. 382During mitosis, interpolar microtubules from two spindle pole bodies (SPBs) interdigitate to create an antiparallel microtubule array for accommodating numerous regulatory proteins. Among these proteins, the kinesin-5 cut7p/Eg5 is the key player responsible for sliding apart antiparallel microtubules and thus helps in establishing the bipolar spindle. At the onset of mitosis, two SPBs are adjacent to one another with most microtubules running nearly parallel toward the nuclear envelope, creating an unfavorable microtubule configuration for the kinesin-5 kinesins. Therefore, how the cell organizes the antiparallel microtubule array in the first place at mitotic onset remains enigmatic. Here, we show that a novel protein psrp1p localizes to the SPB and plays a key role in organizing the antiparallel microtubule array. The absence of psr1+ leads to a transient monopolar spindle and massive chromosome loss. Further functional characterization demonstrates that psr1p is recruited to the SPB through interaction with the conserved SUN protein sad1p and that psr1p physically interacts with the conserved microtubule plus tip protein mal3p/EB1. These results suggest a model that psr1p serves as a linking protein between sad1p/SUN and mal3p/EB1 to allow microtubule plus ends to be coupled to the SPBs for organization of an antiparallel microtubule array. Thus, we conclude that psr1p is involved in organizing the antiparallel microtubule array in the first place at mitosis onset by interaction with SUN/sad1p and EB1/mal3p, thereby establishing the bipolar spindle.postprin

Removal of antagonistic spindle forces can rescue metaphase spindle length and reduce chromosome segregation defects

Regular Abstracts - Tuesday Poster Presentations: no. 1925Metaphase describes a phase of mitosis where chromosomes are attached and oriented on the bipolar spindle for subsequent segregation at anaphase. In diverse cell types, the metaphase spindle is maintained at a relatively constant length. Metaphase spindle length is proposed to be regulated by a balance of pushing and pulling forces generated by distinct sets of spindle microtubules and their interactions with motors and microtubule-associated proteins (MAPs). Spindle length appears important for chromosome segregation fidelity, as cells with shorter or longer than normal metaphase spindles, generated through deletion or inhibition of individual mitotic motors or MAPs, showed chromosome segregation defects. To test the force balance model of spindle length control and its effect on chromosome segregation, we applied fast microfluidic temperature-control with live-cell imaging to monitor the effect of switching off different combinations of antagonistic forces in the fission yeast metaphase spindle. We show that spindle midzone proteins kinesin-5 cut7p and microtubule bundler ase1p contribute to outward pushing forces, and spindle kinetochore proteins kinesin-8 klp5/6p and dam1p contribute to inward pulling forces. Removing these proteins individually led to aberrant metaphase spindle length and chromosome segregation defects. Removing these proteins in antagonistic combination rescued the defective spindle length and, in some combinations, also partially rescued chromosome segregation defects. Our results stress the importance of proper chromosome-to-microtubule attachment over spindle length regulation for proper chromosome segregation.postprin