Go for It! Supporting people with learning disabilities and / or Autistic Spectrum Disorders in Employment

This study, commissioned to inform The same as you? National Implementation Group, looked at what employment support there is in Scotland for people with learning disabilities and/or autistic spectrum disorders (ASD) and identified good practice. The research surveyed providers of employment support, looked at the literature on best practice, gathered opinions about barriers and key issues, and explored experiences and views of supported employees, their families and employers

Cardiovascular, respiratory, gastrointestinal and behavioral effects of intravenous lidocaine in healthy, conscious horses and evaluation of the relationship with lidocaine and monoethylglycinexylidide serum concentrations

This study aimed to evaluate the relationship between the serum concentrations of lidocaine/ monoethylglycinexylidide (MEGX) and their effects on several systems in horses. Five healthy, conscious horses received a two-hour placebo intravenous infusion followed by a two-hour lidocaine infusion (bolus of 1.3 mg/kg over ten minutes followed by a continuous rate infusion of 0.05 mg/kg/min). Lidocaine and MEGX serum concentrations were sampled every ten to fifteen minutes during the experiment, and the presence of muscle fasciculations and loss of balance as well as the respiratory, digestive and cardiovascular systems of the five horses were evaluated by means of different non-invasive methods. During the lidocaine infusion, the mean (f SD) lidocaine and MEGX concentrations were respectively 768.88 +/- 93.32ng/ml and 163.08 +/- 108.98 ng/ml. The infusion of lidocaine significantly influenced the presence of fasciculations, caused a statistically but non-clinically significant decrease of systolic and diastolic blood pressures, which were both correlated with lidocaine and MEGX serum concentrations, and it increased the duodenal contractions frequency, which was correlated with the serum lidocaine concentration. In this study, mild hypotensive and prokinetic effects of short-term lidocaine infusion were observed

Vacuum infusion of natural fibre composites for structural applications

Numerous methods of manufacturing natural fibre composites have been reported in the literature, including compression moudling, often in conjunction with a hot press. Other forms of composite manufacture include 'Vacuum Assisted Resin Transfer Moulding' (VATRM) and the 'Seemann Composite Resin Infusion Moulding Process' (SCRIMP). These methods have been reported to produce natural fibre composies with reasonable mechanical properties [1-2]. In this paper, a vacuum infusion rig is described that has been developed to produce consistent quality composite plates for studies into optimising natural fibre composites. The process aims to harness the benefits of vacuum infusion and compression moulding, where vacuum infusion encourages the removal of trapped air in the system and hence avoid reduction, and additional compression moulding can help to achieve high volume fractions that are otherwise difficult in other processes

Infusion pasteurization of whole milk and skim milk: Influence on viscosity and particle size

Infusion pasteurization was performed on both whole milk and skim milk and at different temperatures in the range 72°C-120°C. The skim milk was prepared at a commercial dairy and had been heated to approx. 60°C during the separation process. The whole milk was skimmed by centrifugation prior to the analyses. In the analyses, the infusion pasteurized samples were compared to a standard low pasteurization on the same batches of milk and samples of the raw milks. Particle sizes were analyzed using dynamic light scattering, and the viscosity of the samples were measured with a capillary viscometer. The viscosity measurements showed no significant changes in viscosity after infusion pasteurization of skim milk, nor did the particle sizes change. On the other hand, when whole milk was infusion pasteurized an increase in viscosity of the skim milk fraction was seen as treatment temperature increased, and an increase in the z-average diameter of particles and broadening of the size distributions was observed. These observations were quite surprising and might be the result of influence of several different processes during and after infusion pasteurization

Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy

Chimeric antigen receptor (CAR) T-cell therapy has produced remarkable anti-tumor responses in patients with B-cell malignancies. However, clonal kinetics and transcriptional programs that regulate the fate of CAR-T cells after infusion remain poorly understood. Here we perform TCRB sequencing, integration site analysis, and single-cell RNA sequencing (scRNA-seq) to profile CD8+ CAR-T cells from infusion products (IPs) and blood of patients undergoing CD19 CAR-T immunotherapy. TCRB sequencing shows that clonal diversity of CAR-T cells is highest in the IPs and declines following infusion. We observe clones that display distinct patterns of clonal kinetics, making variable contributions to the CAR-T cell pool after infusion. Although integration site does not appear to be a key driver of clonal kinetics, scRNA-seq demonstrates that clones that expand after infusion mainly originate from infused clusters with higher expression of cytotoxicity and proliferation genes. Thus, we uncover transcriptional programs associated with CAR-T cell behavior after infusion.Published versio

The effect of exogenous glucose infusion on early embryonic development in lactating dairy cows

peer-reviewedThe objective of this study was to examine the effect of intravenous infusion of glucose on early embryonic development in lactating dairy cows. Nonpregnant, lactating dairy cows (n = 12) were enrolled in the study (276 ± 17 d in milk). On d 7 after a synchronized estrus, cows were randomly assigned to receive an intravenous infusion of either 750 g/d of exogenous glucose (GLUC; 78 mL/h of 40% glucose wt/vol) or saline (CTRL; 78 mL/h of 0.9% saline solution). The infusion period lasted 7 d and cows were confined to metabolism stalls for the duration of the study. Coincident with the commencement of the infusion on d 7 after estrus, 15 in vitro-produced grade 1 blastocysts were transferred into the uterine horn ipsilateral to the corpus luteum. All animals were slaughtered on d 14 to recover conceptuses, uterine fluid, and endometrial tissue. Glucose infusion increased circulating glucose concentrations (4.70 ± 0.12 vs. 4.15 ± 0.12 mmol/L) but did not affect milk production or dry matter intake. Circulating β-hydroxybutyrate concentrations were decreased (0.51 ± 0.01 vs. 0.70 ± 0.01 mmol/L for GLUC vs. CTRL, respectively) but plasma fatty acids, progesterone, and insulin concentrations were unaffected by treatment. Treatment did not affect either uterine lumen fluid glucose concentration or the mRNA abundance of specific glucose transporters in the endometrium. Mean conceptus length, width, and area on d 14 were reduced in the GLUC treatment compared with the CTRL treatment. A greater proportion of embryos in the CTRL group had elongated to all length cut-off measurements between 11 and 20 mm (measured in 1-mm increments) compared with the GLUC treatment. In conclusion, infusion of glucose into lactating dairy cows from d 7 to d 14 post-estrus during the critical period of conceptus elongation had an adverse impact on early embryonic development

Infusion pasteurization of skim milk: Effects of different time-temperature combinations

Infusion pasteurization technology was used in different time-temperature combinations for heat treatment of skim milk and compared to untreated skim milk and a standard pasteurization treatment. Aerobic count of microorganisms and activity of alkaline phosphatase showed that all infusion-pasteurized samples had received proper pasteurization. There were no difference in the size of casein micelles, but differences were seen in activity of the enzyme xanthine oxidase. The results indicate possible differences in properties of infusion-pasteurized skim milk compared to standard pasteurized skim milk

Circulating free fatty acids, insulin, and glucose during chemical stimulation of hypothalamus in rats

The aim of this study was to investigate plasma free fatty acids (FFA), insulin, and blood glucose during chemical stimulation of the lateral and ventromedial hypothalamic areas (LHA and VMH) in rats. Therefore male Wistar rats were implanted with bilateral cannulas in the LHA or the VMH and into the left and right jugular veins. Freely moving rats were then infused into the LHA and VMH with norepinephrine (NE), epinephrine (E), or acetylcholine or intravenously with NE or E. Before, during, and after the infusions, simultaneous blood samples were taken without disturbing the animals. Infusion of NE into the LHA resulted in a decrease of plasma FFA and a simultaneous increase of insulin. NE infusion in the VMH elicited an increase of plasma FFA, plasma insulin, and blood glucose. E infusion into the LHA did not lead to a change of plasma FFA, whereas insulin and glucose showed an increase. E infusion into the VMH evoked increases of plasma FFA and insulin. Peripheral administration of NE led to a sharp increase of FFA, whereas plasma insulin and blood glucose did not change. E in the periphery elicited an augmentation of plasma FFA and blood glucose and a suppression of insulin during infusion. After termination of E infusion, plasma FFA and glucose levels decreased, whereas plasma insulin showed a sharp increase. It is concluded 1) that the effects produced by administration of NE and E are dependent on hypothalamic localization and local receptor population characteristics; 2) that there are striking differences regarding the effects on the investigated blood parameters between hypothalamically infused NE and E and peripherally infused NE and E; and 3) that the LHA and VMH are able to alter plasma FFA levels independently of blood glucose and insulin levels.

UJI EFEKTIFITAS INFUS DAUN SIRIH (Piper betle L) TERHADAP PERTUMBUHAN Colletotrichum gloeosporioides SECARA IN VITRO

There are various disease attacking plant. According to the cause, plant disease divided into two kinds, biotic (parasite) and abiotic (non-parasite). Biotic disease or parasite is disease caused by fungus, bacterial, virus, nematode, another plant or disturbing plant. Abiotic disease or non- parasite is plant disease caused by mineral poisoned, soil deficiency, polution, un-fit environment. Post harvest disease organisme generally caused by fungus or bacterial. Colletorichum sp is a fungi which caused anthraxnose, developed further, caused a brown color in fruit skin. It would made the skin disintegrated, soft, and change its color into brown. The brown spot became larger and darker, and it became rotten. Betel vine infusion, beside has antiseptic ability and anti-oxydant, it also functioned as fungicide, it caused by eugenol in betel vine from phenol which could be useful as disinfectant. This research aimed to find out the influence of betel vine infusion to the growth of Colletotrichum gloeosporioides and also to find out the infusion concentrate which effective in inhibiting the Colletotrichum gloeosporioide development. Parameter used is the diameter of Colletotrichum gloeosporioide development after betel vine infusion. The research was real experiment used Complete Random Design, It was Randomized Control-Group Posttest Design which consist of 8 treatments. They were concentrate 0%, 75%, 80%, 85%, 90%, 95%, 100% and dithane M45 control with 4 times repeat. Subject in this research was Colletotrichum gloeosporioides from Brawijaya University Farming Laboratory. The research was done at Microbiology Division Biology Laboratory University of Muhammadiyah Malang at May 29th to August 10th 2007. Data analysis used one way variant analysis and Duncan’s Test Phase 1%. The research showed that there was influence of betel vine infusion to the development of Colletotrichum gloeosporioides also optimal concentration in inhibiting the development of Colletotrichum gloeosporioides. From Duncan’s phase 1% analysis, could be found that in each concentration showed different aggregate in every treatment and betel vine infusion at concentrate 75% gave the largest effective influence in inhibiting the development of colletotrichum gloeosporioides since the diameter was the smallest

Perioperative donor bone marrow infusion augments chimerism in heart and lung transplant recipients

Background.: We and others have demonstrated that a low level of donor cell chimerism was present for years after transplantation in tissues and peripheral blood of heart and lung recipients; it was associated, in the latter, with a lower incidence of chronic rejection. To augment this phenomenon, we initiated a trial combining simultaneous infusion of donor bone marrow with heart or lung allotransplantation. Methods.: Between September 1993 and January 1995, 15 nonconditioned patients received either heart (n = 10) or lung (n = 5) allografts concurrently with an infusion of unmodified donor bone marrow (3.0 × 108 cells/kg), and were maintained on an immunosuppressive regimen consisting of tacrolimus and steroids. Results.: There was no complication associated with the infusion of donor bone marrow. Chimerism was detectable in 73% of bone marrow-augmented patients up to the last sample tested. Of the 5 control recipients who did not receive bone marrow infusion, only 1 had detectable chimerism by flow on postoperative day 15, which dwindled to an undetectable level by postoperative day 36. None of the patients had evidence of donor-specific immune modulation by mixed lymphocyte reaction. Conclusions.: The combined infusion of donor bone marrow and heart or lung transplantation, without preconditioning of the recipient, is safe and is associated with an augmentation of donor cell chimerism. © 1995 The Society of Thoracic Surgeons