Geometry Processing of Conventionally Produced Mouse Brain Slice Images

Brain mapping research in most neuroanatomical laboratories relies on conventional processing techniques, which often introduce histological artifacts such as tissue tears and tissue loss. In this paper we present techniques and algorithms for automatic registration and 3D reconstruction of conventionally produced mouse brain slices in a standardized atlas space. This is achieved first by constructing a virtual 3D mouse brain model from annotated slices of Allen Reference Atlas (ARA). Virtual re-slicing of the reconstructed model generates ARA-based slice images corresponding to the microscopic images of histological brain sections. These image pairs are aligned using a geometric approach through contour images. Histological artifacts in the microscopic images are detected and removed using Constrained Delaunay Triangulation before performing global alignment. Finally, non-linear registration is performed by solving Laplace's equation with Dirichlet boundary conditions. Our methods provide significant improvements over previously reported registration techniques for the tested slices in 3D space, especially on slices with significant histological artifacts. Further, as an application we count the number of neurons in various anatomical regions using a dataset of 51 microscopic slices from a single mouse brain. This work represents a significant contribution to this subfield of neuroscience as it provides tools to neuroanatomist for analyzing and processing histological data.Comment: 14 pages, 11 figure

Robust point correspondence applied to two and three-dimensional image registration

Accurate and robust correspondence calculations are very important in many medical and biological applications. Often, the correspondence calculation forms part of a rigid registration algorithm, but accurate correspondences are especially important for elastic registration algorithms and for quantifying changes over time. In this paper, a new correspondence calculation algorithm, CSM (correspondence by sensitivity to movement), is described. A robust corresponding point is calculated by determining the sensitivity of a correspondence to movement of the point being matched. If the correspondence is reliable, a perturbation in the position of this point should not result in a large movement of the correspondence. A measure of reliability is also calculated. This correspondence calculation method is independent of the registration transformation and has been incorporated into both a 2D elastic registration algorithm for warping serial sections and a 3D rigid registration algorithm for registering pre and postoperative facial range scans. These applications use different methods for calculating the registration transformation and accurate rigid and elastic alignment of images has been achieved with the CSM method. It is expected that this method will be applicable to many different applications and that good results would be achieved if it were to be inserted into other methods for calculating a registration transformation from correspondence

Online Mutual Foreground Segmentation for Multispectral Stereo Videos

The segmentation of video sequences into foreground and background regions is a low-level process commonly used in video content analysis and smart surveillance applications. Using a multispectral camera setup can improve this process by providing more diverse data to help identify objects despite adverse imaging conditions. The registration of several data sources is however not trivial if the appearance of objects produced by each sensor differs substantially. This problem is further complicated when parallax effects cannot be ignored when using close-range stereo pairs. In this work, we present a new method to simultaneously tackle multispectral segmentation and stereo registration. Using an iterative procedure, we estimate the labeling result for one problem using the provisional result of the other. Our approach is based on the alternating minimization of two energy functions that are linked through the use of dynamic priors. We rely on the integration of shape and appearance cues to find proper multispectral correspondences, and to properly segment objects in low contrast regions. We also formulate our model as a frame processing pipeline using higher order terms to improve the temporal coherence of our results. Our method is evaluated under different configurations on multiple multispectral datasets, and our implementation is available online.Comment: Preprint accepted for publication in IJCV (December 2018

Coercive Region-level Registration for Multi-modal Images

We propose a coercive approach to simultaneously register and segment multi-modal images which share similar spatial structure. Registration is done at the region level to facilitate data fusion while avoiding the need for interpolation. The algorithm performs alternating minimization of an objective function informed by statistical models for pixel values in different modalities. Hypothesis tests are developed to determine whether to refine segmentations by splitting regions. We demonstrate that our approach has significantly better performance than the state-of-the-art registration and segmentation methods on microscopy images.Comment: This work has been accepted to International Conference on Image Processing (ICIP) 201