Delimiting Cryptic Morphological Variation among Human Malaria Vector Species using Convolutional Neural Networks

Deep learning is a powerful approach for distinguishing classes of images, and there is a growing interest in applying these methods to delimit species, particularly in the identification of mosquito vectors. Visual identification of mosquito species is the foundation of mosquito-borne disease surveillance and management, but can be hindered by cryptic morphological variation in mosquito vector species complexes such as the malaria-transmitting Anopheles gambiaecomplex. We sought to apply Convolutional Neural Networks (CNNs) to images of mosquitoes as a proof-of-concept to determine the feasibility of automatic classification of mosquito sex, genus, species, and strains using whole-body, 2D images of mosquitoes. We introduce a library of 1, 709 images of adult mosquitoes collected from 16 colonies of mosquito vector species and strains originating from five geographic regions, with 4 cryptic species not readily distinguishable morphologically even by trained medical entomologists. We present a methodology for image processing, data augmentation, and training and validation of a CNN. Our best CNN configuration achieved high prediction accuracies of 96.96% for species identification and 98.48% for sex. Our results demonstrate that CNNs can delimit species with cryptic morphological variation, 2 strains of a single species, and specimens from a single colony stored using two different methods. We present visualizations of the CNN feature space and predictions for interpretation of our results, and we further discuss applications of our findings for future applications in malaria mosquito surveillance

Robust, Resilient Networked Communication in Challenged Environments

In challenged environments, digital communication infrastructure may be difficult or even impossible to access. This is especially true in rural and developing regions, as well as in any region during a time of political or environmental crisis. We advance the state of the art in wireless networking and security to design networks and applications that rapidly assess changing networking conditions to restore communication and provide local situational awareness. This dissertation examines new systems for responding to current and emerging needs for wireless networks. This work looks across the wireless ecosystem of widely deployed standards. We develop new tools to improve network assessment and to provide robust and reliable network communication. By incorporating new technological breakthroughs, such as the wide commercial success of Unmanned Aircraft Systems (UAS), we introduce novel methods and systems for existing wireless standards for these challenged networks. We assess how existing technologies and standards function in difficult environments: lacking end-end Internet connectivity, experiencing overload or other resource constraints, and operating in three dimensional space. Through this lens, we demonstrate how to optimize networks to serve marginalized communities outside of first world urban cities and make our networks resilient to natural and political crisis that threaten communication

Generation of a Land Cover Atlas of environmental critic zones using unconventional tools

L'abstract è presente nell'allegato / the abstract is in the attachmen