7,459 research outputs found

    Educating Sub-Saharan Africa:Assessing Mobile Application Use in a Higher Learning Engineering Programme

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    In the institution where I teach, insufficient laboratory equipment for engineering education pushed students to learn via mobile phones or devices. Using mobile technologies to learn and practice is not the issue, but the more important question lies in finding out where and how they use mobile tools for learning. Through the lens of Kearney et al.’s (2012) pedagogical model, using authenticity, personalisation, and collaboration as constructs, this case study adopts a mixed-method approach to investigate the mobile learning activities of students and find out their experiences of what works and what does not work. Four questions are borne out of the over-arching research question, ‘How do students studying at a University in Nigeria perceive mobile learning in electrical and electronic engineering education?’ The first three questions are answered from qualitative, interview data analysed using thematic analysis. The fourth question investigates their collaborations on two mobile social networks using social network and message analysis. The study found how students’ mobile learning relates to the real-world practice of engineering and explained ways of adapting and overcoming the mobile tools’ limitations, and the nature of the collaborations that the students adopted, naturally, when they learn in mobile social networks. It found that mobile engineering learning can be possibly located in an offline mobile zone. It also demonstrates that investigating the effectiveness of mobile learning in the mobile social environment is possible by examining users’ interactions. The study shows how mobile learning personalisation that leads to impactful engineering learning can be achieved. The study shows how to manage most interface and technical challenges associated with mobile engineering learning and provides a new guide for educators on where and how mobile learning can be harnessed. And it revealed how engineering education can be successfully implemented through mobile tools

    Masculinities, vulnerability and negotiated identity: Understanding the reporting behaviours of men who experience violence or otherwise harmful behaviour, within a sex work context

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    Context The focus of sex work related discussions most commonly falls on female providers of sexual services, and male purchasers. As a result, the often victim-oriented policy response in England and Wales falls short of truly addressing the needs of men who are involved in the sale of sex, with there being limited support available for them and a systemic approach which does not fully recognise the potential for men to face harm within this context. Methods The aim of this study is to explore experiences of and reactions to violence, and otherwise harmful behaviours, faced by men in the context of their sex working, by understanding the lived realities of a sample of men who engage in this type of work. The study takes a phased approach which combines an initial informative quantitative survey, with three subsequent phases of semi-structured interviews with male sex workers, sex work-focused practitioners and police officers. The method is guided by feminist research principles which suggest that reality is situated within those with lived experience, and also by an element of co-creation which has grounded this study within the perspectives of male sex workers from its conception. Findings The findings of this research suggest that all of the men involved in the study had faced at least one of the violent or otherwise harmful behaviours outlined, though reporting of these behaviours was not at all common. Discussions with the male sex working participants, practitioners and the police highlighted the issues related to the structural influences of authority, such as the police, and the social environment, and the internalisation of these wider factors, which create barriers to reporting for groups such as male sex workers and others who face similar social marginalisation. Conclusions This study challenges existing gendered understandings of violence and otherwise harmful behaviour within a sex work context, by highlighting the harmful experiences of men. By exploring these experiences and the reporting behaviours of those involved, the study also proposes a new framework for understanding barriers to reporting, which suggests that these are formed through the influences of formal and informal measures of social control, and the internalisation of these outside influences by the individual. By better understanding the experiences of men, and the barriers to their reporting, this study attempts to nuance a gendered discussion. Within, I propose that in order to better support male sex workers, responses must begin by appreciating the heterogeneity of those involved in sex work and the influence of their individual circumstances and the social environment on their willingness to seek support

    The Politics of Intermediality: Late Modernist Circulations of the Event

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    This dissertation examines late modernist, intermedial representations of events, considering art as an event and how art depicts and circulates events. Through cross-media close readings and interdisciplinary theories and methods derived from media studies, music and sound studies, intermedial theory, feminist theory, critical race theory, and literary theory, I study multimedia opera, civilian bombardments during the Spanish Civil War, the 1943 Harlem riot, and the atomic bombing of Japan in order to evaluate media practices from a range of cultural and historical contexts. Employing eventalization, my research illuminates intersections of media, gender, race, nation, and sexuality. Some of the artists I engage with include Gertrude Stein, Virgil Thomson, Virginia Woolf, Pablo Picasso, Dora Maar, Langston Hughes, Jacob Lawrence, Ann Petry, Mina Loy, John Hersey, Shda Shinoe, and Nagai Takashi. The four chapters comprising this project take up fluctuating interactions among sonic, verbal, and visual mediations that were produced between 1927 and 1949, juxtaposing various newer media (photojournalism, radio, and others) with works of art (poetry, fiction, and painting). Stein and Thomson's Four Saints in Three Acts, transposed first into a staged opera and then as a radio broadcast, highlights how its many remediations offer formal innovation while reinforcing historical inequities. Picasso and Woolf's collage-like responses to war in Spain demonstrate hypermediacy and immediacy—remediation's twinned impulses—with each artist treating public and private divisions (as materials and as politics) differently. In their depictions of state violence against Black Americans, Hughes, Lawrence, and Petry draw differently on sonic, visual, and verbal modes. Hughes and Petry's fictional rioters publicly express dissatisfaction and challenge the containment strategies used during the actual riot. My concluding chapter also considers how intermediality resists containment, tracing the disparate availability of media in North America and Japan. Simultaneously empty and excessive, these atomic media reveal the ways knowledge and power produce nuclear subjects. My findings reveal that late modernism offers a particularly resonant set of texts and contexts from which to evaluate literature as a medium. Moreover, literature's porous borders enable multiple movements and engagements. The eventalization of these circulations reveal the political stakes and uses of intermediality

    Examining the Potential for Isotope Analyses of Carbon, Nitrogen, and Sulphur in Burned Bone from Experimental and Archaeological Contexts.

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    The aim of this project was to determine whether isotope analyses of carbon, nitrogen and sulphur can be conducted on collagen extracted from burned bone. This project was conducted in two phases: a controlled heating experiment and an archaeological application. The controlled heating experiment used cow (Bos taurus) bone to test the temperature thresholds for the conservation of δ13C, δ15N, and δ34S values. These samples were also used to test the efficacy of Fourier Transform Infrared spectroscopy (FTIR) and colour analysis, for determining the burning intensities experienced by bone burned in unknown conditions. The experiment showed that δ13C values were relatively unchanged up to 400°C (<2‰ variation), while δ15N values were relatively stable up to 200°C (0.5‰ variation). Values of δ34S were also relatively stable up to 200°C (1.4‰ variation). Colour change and FTIR data were well correlated with the change in isotope ratios. Models estimating burning intensities were created from the FTIR data. For the archaeological application, samples were selected from two early Anglo-Saxon cemetery sites: Elsham and Cleatham. Samples were selected from both inhumed and cremated individuals. Among the inhumed individuals δ13C values suggested a C3 terrestrial diet and δ15N values suggested protein derived largely from terrestrial herbivores, as expected for the early Anglo-Saxon period. However, δ34S values suggested the consumption of freshwater resources and that this consumption was related to both the age and sex of the individual. The experimental data shows that there is potential for isotope analyses of cremated remains, as during the cremation process heat exposures are not uniform across the body. The samples selected for the archaeological application, however, were not successful. Bone samples heated in controlled conditions produced viable collagen for isotope analysis; however, there are several differences between experiments conducted in a muffle furnace and open-air pyre cremation that need to be investigated further. Additionally, the influence of taphonomy on collagen survival in burned bone needs to be quantified. Finally, methods of sample selection need to be improved to find bone samples from archaeologically cremated remains that are most likely to retain viable collagen. While there is significant research that must be conducted before this research can be widely applied there are a multitude of cultures that practised cremation throughout history and around the world that could be investigated through the analyses proposed in this project

    Breaking Ub with Leishmania mexicana: a ubiquitin activating enzyme as a novel therapeutic target for leishmaniasis

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    Leishmaniasis is a neglected tropical disease, which inflicts a variety of gruesome pathologies on humans. The number of individuals afflicted with leishmaniasis is thought to vary between 0.7 and 1.2 million annually, of whom it is estimated that 20 to 40 thousand die. This problem is exemplary of inequality in healthcare – current leishmaniasis treatments are inadequate due to toxicity, cost, and ineffectiveness, so there is an urgent need for improved chemotherapies. Ubiquitination is a biochemical pathway that has received attention in cancer research. It is the process of adding the ubiquitin protein as a post-translational modification to substrate proteins, using an enzymatic cascade comprised of enzymes termed E1s, E2s, and E3s. Ubiquitination can lead to degradation of substrate proteins, or otherwise modulate their function. As the name suggests, this modification can be found across eukaryotic cell biology. As such, interfering with ubiquitination may interfere with essential biological processes, which means ubiquitination may present a new therapeutic target for leishmaniasis. Before ubiquitination inhibitors can be designed, components of the ubiquitination system must be identified. To this end, a bioinformatic screening campaign employed BLASTs and hidden Markov models, using characterised orthologs from model organisms as bait, to screen publicly-available Leishmania mexicana genome sequence databases, searching for genes encoding putative E1s, E2s, and E3s. To confirm some of these identifications on a protein level, activity-based probes, protein pulldowns, and mass spectrometry were used. Using an activity-based probe that emulates the structure of adenylated ubiquitin, E1s were identified, and their relative abundance quantified. A chemical crosslinker extended the reach of this probe, allowing the identification of an E2 (LmxM.33.0900). It is noted that L. mexicana has two E1s – unusual for a single celled organism. Of these E1s, LmxM.34.3060 was considerably more abundant than LmxM.23.0550 in both major life cycle stages of the in vitro Leishmania cultures. It is important to describe the wider context of these enzymes – what is their interactome, what are their substrates? To study this, CRISPR was used to fuse a proximity-based labelling system, BioID, on genes of interest – LmxM.34.3060 and LmxM.33.0900. The E2 (LmxM.33.0900) was shown to interact with the E1 (LmxM.34.3060), validating the results from the activity-based probe and crosslinker experiments. Due to sequence homology with characterised orthologs, the E2 was hypothesised to function in the endoplasmic reticulum degradation pathway. Immunoprecipitations of a ubiquitin motif, diglycine, were conducted with a view to gathering information on the substrates of ubiquitin. Anti-diglycine peptides included some of those identified by BioID. Experiments examining ubiquitin’s role in the DNA damage response were also initiated, as were improvements to the proximity-based labelling system, however these were not followed to completion due to a lack of time and resources. To examine the possibility of finding novel drug targets in the ubiquitination cascade, recombinant proteins were expressed. LmxM.34.3060 was expressed in a functional form, while a putative SUMO E2 (LmxM.02.0390) was functional after refolding. Expressed LmxM.33.0900 was not functional and could not be refolded into a functional form. Drug assays were conducted on LmxM.34.3060, which found an inhibitor of the human ortholog, TAK-243, to be 20-fold less effective against the Leishmania enzyme. Additional assays found an inhibitor that was 50-fold more effective at inhibiting the Leishmania enzyme as opposed to its human equivalent - 5'O-sulfamoyl adenosine. Furthermore, a new mechanism of action, inhibiting the E1, for was identified for drugs previously characterised to inhibit protein synthesis. LmxM.34.3060 underwent biophysical characterisation, with structural information obtained using SAXS and protein crystallography. A crystal structure was solved to 3.1 Å, with the in-solution SAXS structure complementary to this. TAK-243 was modelled into the LmxM.34.3060 structure and clashes were predicted, concurring with TAK-243’s reduced efficacy against the Leishmania enzyme in the drug assays. This project aimed to characterise the potential of an understudied biochemical system to provide novel therapeutic targets for a neglected tropical pathogen. To achieve this aim it presents the identifications of two E1s, an interactome, a structure, and a potent, selective inhibitor of a Leishmania ubiquitin activating enzyme

    Going sideways: The poetics of queer-becoming in India

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    Liberation movements based on identity present themselves with enormous challenges regarding rights and inequalities. The Indian public health response to HIV/AIDS resulted in foregrounding the existing identity ‘Kothi’ and adopting a behavioural category called ‘MSM’ to designate the main beneficiaries of aid. This underprivileged part of the population was consequently provided with victim narratives which explains why it is impossible to render its stories without pathos. Linked to a quest for emancipation, identity is a complex category especially in post-colonial nation-states like India. Here identities are unavoidably entangled with other modes of governance such as religion, caste, class, and the law. The socioeconomic and geopolitical realities of the Kothis confine them to HIV/AIDS discourse, where they continue to be labelled as subaltern queers. Failing to embrace the specificities of identities, this discourse erases differences and inevitably undermines the very creative and liveliness of what can become queer. Going Sideways is a practice-led inquiry that problematises the neo-liberal, neo-colonial approaches to identity politics, while resisting the sense of concerted queerness that occurred in the form of already-known prescriptions to assimilate all subjects of desire in India, thereby discounting historical and social inequalities. Going sideways offers a threefold framework which builds its artistic resistances through critical fabulation and storytelling as minor forms of literature that in-turn demand a listening-encounter which enables a queer possibility, a leap into the future. The threefold braid consists of stories that function as an auto-collective-biography of the Kothis, a glossary as a non-closed, living-breathing archive that politically and conceptually binds this thesis, and a moving image work which helps to visualise this project empathetically, but also creates a political bent. This project seeks creative ecstasy through storytelling which prepares for an encounter to happen. Together these aphoristic approaches create a gentler ‘knowledge-system’ as a ‘rehearsal,’ invoking the essence of becoming. It allows a queer-wisdom to be shared, and its orality enables us to hear the mumble of the unvoiced. Sideways thinking has an erotic energy which brings us to life in a queer way, without being categorised. This work also marks an urgency to rethink identity politics subversively, by inhabiting a path that is always-already sideways. It is a work of love and friendship, that calls for radical and ethical equality. This resituates the poetics of Queer-Becoming in the field of emergence, where one can imagine new ways of encounters in a queer multiverse

    Examining the opportunities for agricultural experiences as part of Scottish secondary school pupils’ learning under Curriculum for Excellence

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    Society is increasingly disconnected from the processes and practices of agriculture as food production, and therefore the true cost and value of food. A way in which to overcome this disconnect would be to increase agricultural literacy levels through education. Learning outside the classroom has been shown to benefit children and young people including personal development and increased care towards the environment. Sustainability learning, including outdoor learning, as an approach to developing sustainable behaviours is the focus of much research. There is, however, a gap in research on the potential for agricultural learning experiences that demonstrate the positive role agriculture plays within global environmental systems. Agriculture is often portrayed in a negative framing in regard to the impacts of human action on the environment. The aim of this study was to examine opportunities for Curriculum for Excellence (CfE) to deliver lasting impressions of farming and food production for secondary school pupils in Scotland through a concept of agricultural experiences. This research was conducted through qualitatively-driven mixed methods consisting of survey, interview, and focus group methodology with a range of school-based and rural-based participants. The research found that CfE inadvertently maintains an anti-rural position, reflected in the lack of any meaningful reference to agriculture within the Experiences and Outcomes; framing agriculture within a context of negative environmental impact. There exist clear opportunities, as well multiple benefits, for agricultural experiences under CfE learning, however, there remain challenges for implementation within current CfE cultures and structures. Pupils and teachers recognised the value of agricultural experiences to deliver meaningful experiential learning experiences, as well as developing knowledge and skills for lifelong learning. Scottish agricultural stakeholders and farmers feel that media misrepresentation contributes to societal disconnect and thus the attitudes and perceptions of agriculture, particularly livestock farming, while often negating to recognise the primary function of agriculture-as-food within the current challenges facing global environmental systems. Five recommendations are put forward as a result of this research: Words Matter, Framing Farming, Balanced Environmental Education, Build Partnerships, and Be Bold. These capture ways in which agriculture and agricultural experiences can be better incorporated through a ‘Minimum Effort Strategy’ which would strengthen current CfE structures, and a ‘Radical Strategy’ which envisions a planetary or agricultural phronesis challenging us to a transformation in sustainability learning that re-imagines our human relation to the world

    Investigation of Rabs and cargo receptors as key regulators of vesicle traffic

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    Eukaryotic cells are subdivided into organelles with unique physicochemical compositions and functions. Establishment and maintenance of these organelles requires selective exchange of material by vesicle trafficking. Transport vesicles are formed by cytoplasmic coat protein complexes which shape the membrane, and play a further role in the selection of transmembrane cargo proteins. Transmembrane cargo receptors select cargo in the luminal compartment and bind to these coat proteins through signals in their cytoplasmic domains. Once formed, vesicles recognise and fuse with a target organelle to deliver their content. This final process is tightly regulated by small GTPases of the Rab superfamily. Here, I will describe my work investigating the role of specific Rab GTPase pathways and the KDEL cargo receptor in trafficking in the secretory and endocytic pathways in mammalian cells. There are over 60 Rabs in human cells, each of which is activated by a specific GDP-GTP exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP) at a defined membrane location. For many of these Rabs the GEF and GAP were unknown at the outset of this work in which I have identified and characterised two structurally related families of Rab GEFs, the DENN and tri-longin domain proteins. The DENN family, expressed only in metazoans, which has 17 members acting on 10 different Rabs. The tri-longin family, comprises 3 heterodimeric GEF complexes: Mon1-Ccz1 for the late endosomal, lysosomal Rab7, Hps1-Hps4 (BLOC-3) which activates Rab32/Rab38 in melanogenesis and Intu-Fuz, part of CPLANE complex, which activates Rab23 at cilia. Additionally, I have shown that the unrelated Rab3GAP complex is a GEF for the ancestral Rab18 regulating the structure of the endoplasmic reticulum. In the final part of this work, I have explored how the integrity of the early secretory system depends on selective export and retrieval of proteins between the ER and the Golgi. An essential component of this system is the transmembrane cargo receptor for KDEL retrieval signals. Here I describe the structure of the KDEL receptor and mechanism by which KDEL cargo binding triggers a conformational change exposing a signal for the COP I vesicle coat
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