Maintenance management process model for school buildings: an application of IDEF0 modelling methodology

The lack of a clear understanding of the maintenance management process is one of the major sources of difficulties in the maintenance of school buildings. A clearer understanding of the maintenance management process can be achieved by constructing a process model of the existing practices using a suitable process modelling technique. The purpose of this study was to develop a process model for the management of maintenance of school buildings using the IDEF0 structured modelling technique. The modelling process is divided into three phases, (i) the information gathering phase, (ii) the model development phase and (ii) the experts' evaluation and validation phase. In the first phase, information on existing maintenance practices was obtained through questionnaires and document analysis of policies, standing orders and maintenance reports. In the second phase, a process model was drafted through an iterative process using the IDEF0 process modelling technique. In the third phase, the draft process model was submitted to three experts on maintenance management from the Ministry of Education Malaysia for evaluation and validation. A ready to implement process model for the maintenance management of school buildings was constructed upon validation by the experts

Identification of fragmented JPEG files in the absence of file systems

Identifying fragmented and deleted files from scattered digital storage become crucial needs in computer forensic. Storage media experience regular space fragmentation which gives direct consequence to the files system series. This paper specifies a case where the jpeg files are heavily fragmented with absent file header which contains maximum information for the stored data can be easily retrieved. The problem is formulated using statistical byte frequency analysis for identifying the group of jpeg file fragments. Several related works have addressed the issue of classifying variety types of file format with high occurrence of being fragmented such as avi, doc, wav file and etc. These files have been tagged as among the larger file format. We provide techniques for identifying the pattern of file fragments distribution and describe roles of selected clustering attributes. Finally, we provide experimental results presenting that the jpeg fragments distribution can be retrieved with quite small gap differences between the groups

Development of a micro-extruder with vibration mode for microencapsulation of human keratinocytes in calcium alginate

Microencapsulation is a promising technique to form microtissues. The existing cell microencapsulation technologies that involved extrusion and vibration are designed with complex systems and required the use of high energy. A micro-extruder with an inclusion of simple vibrator that has the commercial value for creating a 3D cell model has been developed in this work. This system encapsulates human keratinocytes (HaCaT) in calcium alginate and the size of the microcapsules is controllable in the range of 500-800 µm by varying the flow rates of the extruded solution and frequency of the vibrator motor ( I 0-63 Hz). At 0.13 ml/min of flow rate and vibration rate of 26.4 Hz, approximately 40 ± IO pieces of the alginate microcapsules in a size 632.14 ± I 0.35 µm were produced. Approximately I 00 µm suspension of cells at different cells densities of 1.55 x I 05 cells/ml and 1.37 x I 07 cells/ml were encapsulated for investigation of microtissues formation. Fourier transform infrared spectroscopy (FTIR) analysis showed the different functional groups and chemistry contents of the calcium alginate with and without the inclusion of HaCaT cells in comparison to the monolayers of HaCaT cells. From Field Emission Scanning Electron Microscope (FESEM) imaging, calcium alginate microcapsules were characterised by spherical shape and homogenous surface morphology. Via the nuclei staining, the distance between cells was found reduced as the incubation period increased. This indicated that the cells merged into microtissues with good cell-cell adhesions. After 15 days of culture, the cells were still viable as indicated by the fluorescence green expression of calcein­acetoxymethyl. Replating experiment indicated that the cells from the microtissues were able to migrate and has the tendency to form monolayer of cells on the culture flask. The system was successfully developed and applied to encapsulate cells to produce 3D microtissues

Development of a micro-extruder with vibration mode for microencapsulation of human keratinocytes in calcium alginate

Microencapsulation is a promising technique to form microtissues. The existing cell microencapsulation technologies that involved extrusion and vibration are designed with complex systems and required the use of high energy. A micro-extruder with an inclusion of simple vibrator that has the commercial value for creating a 3D cell model has been developed in this work. This system encapsulates human keratinocytes (HaCaT) in calcium alginate and the size of the microcapsules is controllable in the range of 500-800 µm by varying the flow rates of the extruded solution and frequency of the vibrator motor ( I 0-63 Hz). At 0.13 ml/min of flow rate and vibration rate of 26.4 Hz, approximately 40 ± IO pieces of the alginate microcapsules in a size 632.14 ± I 0.35 µm were produced. Approximately I 00 µm suspension of cells at different cells densities of 1.55 x I 05 cells/ml and 1.37 x I 07 cells/ml were encapsulated for investigation of microtissues formation. Fourier transform infrared spectroscopy (FTIR) analysis showed the different functional groups and chemistry contents of the calcium alginate with and without the inclusion of HaCaT cells in comparison to the monolayers of HaCaT cells. From Field Emission Scanning Electron Microscope (FESEM) imaging, calcium alginate microcapsules were characterised by spherical shape and homogenous surface morphology. Via the nuclei staining, the distance between cells was found reduced as the incubation period increased. This indicated that the cells merged into microtissues with good cell-cell adhesions. After 15 days of culture, the cells were still viable as indicated by the fluorescence green expression of calcein­acetoxymethyl. Replating experiment indicated that the cells from the microtissues were able to migrate and has the tendency to form monolayer of cells on the culture flask. The system was successfully developed and applied to encapsulate cells to produce 3D microtissues