Formulating efficient software solution for digital image processing system

© 2015 John Wiley & Sons, Ltd. Digital image processing systems are complex, being usually composed of different computer vision libraries. Algorithm implementations cannot be directly used in conjunction with algorithms developed using other computer vision libraries. This paper formulates a software solution by proposing a processor with the capability of handling different types of image processing algorithms, which allow the end users to install new image processing algorithms from any library. This approach has other functionalities like capability to process one or more images, manage multiple processing jobs simultaneously and maintain the manner in which an image was processed for later use. It is a computational efficient and promising technique to handle variety of image processing algorithms. To promote the reusability and adaptation of the package for new types of analysis, a feature of sustainability is established. The framework is integrated and tested on a medical imaging application, and the software is made freely available for the reader. Future work involves introducing the capability to connect to another instance of processing service with better performance

A novel automated rodent tracker (ART), demonstrated in a mouse model of amyotrophic lateral sclerosis.

Background Generating quantitative metrics of rodent locomotion and general behaviours from video footage is important in behavioural neuroscience studies. However, there is not yet a free software system that can process large amounts of video data with minimal user interventions. New method Here we propose a new, automated rodent tracker (ART) that uses a simple rule-based system to quickly and robustly track rodent nose and body points, with minimal user input. Tracked points can then be used to identify behaviours, approximate body size and provide locomotion metrics, such as speed and distance. Results ART was demonstrated here on video recordings of a SOD1 mouse model, of amyotrophic lateral sclerosis, aged 30, 60, 90 and 120 days. Results showed a robust decline in locomotion speeds, as well as a reduction in object exploration and forward movement, with an increase in the time spent still. Body size approximations (centroid width), showed a significant decrease from P30. Comparison with existing method(s) ART performed to a very similar accuracy as manual tracking and Ethovision (a commercially available alternative), with average differences in coordinate points of 0.6 and 0.8 mm, respectively. However, it required much less user intervention than Ethovision (6 as opposed to 30 mouse clicks) and worked robustly over more videos. Conclusions ART provides an open-source option for behavioural analysis of rodents, performing to the same standards as commercially available software. It can be considered a validated, and accessible, alternative for researchers for whom non-invasive quantification of natural rodent behaviour is desirable