6,650 research outputs found

    Animating potential for intensities and becoming in writing: challenging discursively constructed structures and writing conventions in academia through the use of storying and other post qualitative inquiries

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    Written for everyone ever denied the opportunity of fulfilling their academic potential, this is ‘Chloe’s story’. Using composite selves, a phrase chosen to indicate multiplicities and movement, to story both the initial event leading to ‘Chloe’s’ immediate withdrawal from a Further Education college and an imaginary second chance to support her whilst at university, this Deleuzo-Guattarian (2015a) ‘assemblage’ of post qualitative inquiries offers challenge to discursively constructed structures and writing conventions in academia. Adopting a posthuman approach to theorising to shift attention towards affects and intensities always relationally in action in multiple ‘assemblages’, these inquiries aim to decentre individual ‘lecturer’ and ‘student’ identities. Illuminating movements and moments quivering with potential for change, then, hoping thereby to generate second chances for all, different approaches to writing are exemplified which trouble those academic constraints by fostering inquiry and speculation: moving away from ‘what is’ towards ‘what if’. With the formatting of this thesis itself also always troubling the rigid Deleuzo-Guattarian (2015a) ‘segmentary lines’ structuring orthodox academic practice, imbricated in these inquiries are attempts to exemplify Manning’s (2015; 2016) ‘artfulness’ through shifts in thinking within and around an emerging PhD thesis. As writing resists organising, the verb thesisising comes into play to describe the processes involved in creating this always-moving thesis. Using ‘landing sites’ (Arakawa and Gins, 2009) as a landscaping device, freely creating emerging ‘lines of flight’ (Deleuze and Guattari, 2015a) so often denied to students forced to adhere to strict academic conventions, this ‘movement-moving’ (Manning, 2014) opens up opportunities for change as in Manning’s (2016) ‘research-creation’. Arguing for a moving away from writing-representing towards writing-inquiring, towards a writing ‘that does’ (Wyatt and Gale, 2018: 127), and toward writing as immanent doing, it is hoped to animate potential for intensities and becoming in writing, offering opportunities and glimmerings of the not-yet-known

    Metaphors of London fog, smoke and mist in Victorian and Edwardian Art and Literature

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    Julian Wolfreys has argued that after 1850 writers employed stock images of the city without allowing them to transform their texts. This thesis argues, on the contrary, that metaphorical uses of London fog were complex and subtle during the Victorian and Edwardian periods, at least until 1914. Fog represented, in particular, formlessness and the dissolution of boundaries. Examining the idea of fog in literature, verse, newspaper accounts and journal articles, as well as in the visual arts, as part of a common discourse about London and the state of its inhabitants, this thesis charts how the metaphorical appropriation of this idea changed over time. Four of Dickens's novels are used to track his use of fog as part of a discourse of the natural and unnatural in individual and society, identifying it with London in progressively more negative terms. Visual representations of fog by Constable, Turner, Whistler, Monet, Markino, O'Connor, Roberts and Wyllie and Coburn showed an increasing readiness to engage with this discourse. Social tensions in the city in the 1880s were articulated in art as well as in fiction. Authors like Hay and Barr showed the destruction of London by its fog because of its inhabitants' supposed degeneracy. As the social threat receded, apocalyptic scenarios gave way to a more optimistic view in the work of Owen and others. Henry James used fog as a metaphorical representation of the boundaries of gendered behaviour in public, and the problems faced by women who crossed them. The dissertation also examines fog and individual transgression, in novels and short stories by Lowndes, Stevenson, Conan Doyle and Joseph Conrad. After 1914, fog was no more than a crude signifier of Victorian London in literature, film and, later, television, deployed as a cliche instead of the subtle metaphorical idea discussed in this thesis

    The Adirondack Chronology

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    The Adirondack Chronology is intended to be a useful resource for researchers and others interested in the Adirondacks and Adirondack history.https://digitalworks.union.edu/arlpublications/1000/thumbnail.jp

    Photography and Aesthetics: a critical study on visual and textual narratives in the lifework of Sergio LarraĂ­n and its impact in 20th century Europe and Latin America

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    The main focus of this study is a theoretical exploration of critical approaches applicable to the work of the Chilean photographer Sergio LarraĂ­n (1931-2012). It presents analytical tools to contextualise and understand the importance and impact of his work in photographic studies and his portrayal of twentieth-century Latin American and European culture. It inspects in depth a large portion of his photo work, which is still only partially published and mostly reduced to his "active" period as a photojournalist, aside from the personal photographic exploration of his early and late career (C. Mena). This extended material creates a broader scope for understanding his photographs and him as a canonical photographer. This study analyses the photographer's trajectory as discourses of recollection of historical memory in time (Mauad) to trace LarraĂ­n's collective memory associated with his visual production. Such analysis helps decode his visual imagery and his projection and impact on the European and Latin American culture. This strategy helps solve a two fold problem: firstly, it generates an interpretive consistency to understand the Chilean's photographic practice; secondly, it explores the power of images as an aesthetic experience in the installation of nationalist ideologies and the creation of imaginaries (B. Anderson 163)

    Cis-Regulation of Gremlin1 Expression during Mouse Limb Bud Development and its Diversification during Vertebrate Evolution

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    Embryonic development and organogenesis rely on tightly controlled gene expression, which is achieved by cis-regulatory modules (CRMs) interacting with distinct transcription factors (TFs) that control spatio-temporal and tissue-specific gene expression. During organogenesis, gene regulatory networks (GRNs) with selfregulatory feedback properties coordinately control growth and patterning and provide systemic robustness against genetic and/or environmental perturbations. During limb bud development, various interlinked GRNs control outgrowth and patterning along all three limb axes. A paradigm network is the epithelial-mesenchymal (e-m) SHH/GREM1/AER-FGF feedback signaling system which controls limb bud outgrowth and digit patterning. The BMP antagonist GREMLIN1 (GREM1) is central to this e-m interactions as its antagonism of BMP activity is essential to maintain both AER-Fgf and Shh expression. In turn, SHH signaling upregulates Grem1 expression, which results in establishment of a self-regulatory signaling network. One previous study provided evidence that several CRMs could regulate Grem1 expression during limb bud development. However, the cis-regulatory logics underlying the spatio-temporal regulation of the Grem1 expression dynamics remained obscure. From an evolutionary point of view, diversification of CRMs can result in diversification of gene regulation which can drive the establishment of morphological novelties and adaptions. This was evidenced by the observed differences in Grem1 expression in different species that correlates with the evolutionary plasticity of tetrapod digit patterning. Hence, a better understanding of spatio-temporal regulation of the Grem1 expression dynamics and underlying cis-regulatory logic is of interest from both adevelopmental and an evolutionary perspective. Recently, multiple candidate CRMs have been identified that might be functionally relevant for Grem1 expression during mouse limb bud development. For my PhD project, I genetically analyzed which of these CRMs are involved in the regulation of the spatial-temporal Grem1 expression dynamics in limb buds. Therefore, we generated various single and compound CRM mutant alleles using CRISPR/Cas9. Our CRMs allelic series revealed a complex Grem1 cis-regulation among a minimum of six CRMs, where a subset of CRMs regulates Grem1 transcript levels in an additive manner. Surprisingly, phenotypic robustness depends not on threshold transcript levels but the spatial integrity of the Grem1 expression domain. In particular, interactions among five CRMs control the characteristic asymmetrical and posteriorly biased Grem1 expression in mouse limb buds. Our results provide an example of how multiple seemingly redundant limb-specific CRMs provide phenotypical robustness by cooperative/synergistic regulation of the spatial Grem1 expression dynamics. Three CRMs are conserved along the phylogeny of extant vertebrates with paired appendages. Of those, the activities of two CRMs recapitulate the major spatiotemporal aspects of Grem1 expression in mouse limb buds. In order to study their functions in species-specific regulation of Grem1 expression and their functional diversification in tetrapods, I tested the orthologous of both CRMs from representative species using LacZ reporter assays in transgenic mice, in comparison to the endogenous Grem1 expression in limb buds of the species of origin. Surprisingly, the activities of CRM orthologues display high evolutionary plasticity, which correlates better with the Grem1 expression pattern in limb buds of the species of origin than its mouse orthologue. This differential responsiveness to the GRNs in mouse suggests that TF binding site alterations in CRMs could underlie the spatial diversification of Grem1 in limb buds during tetrapod evolution. While the fish fin and tetrapod limb share some homologies of proximal bones, the autopod is a neomorphic feature of tetrapods. The Grem1 requirement for digit patterning and conserved expression in fin buds prompted us to assess the enhancer activity of fish CRM orthologues in transgenic mice. Surprisingly, all tested fish CRMs are active in the mouse autopod primordia providing strong evidence that Grem1 CRMs are active in fin buds and that they predate the fin-to-limb transition. Our results corroborate increasing evidence that CRMs governing autopodial gene expression have been co-opted during the emergence of tetrapod autopod. Furthermore, as part of a collaboration with Dr. S. Jhanwar, I contributed to the study of shared and species-specific epigenomic and genomic variations during mouse and chicken limb bud development. In this analysis, Dr. S. Jhanwar identified putative enhancers that show higher chicken-specific sequence turnover rates in comparison to their mouse orthologues, which defines them as so-called chicken accelerated regions (CARs). Here, I analyzed the CAR activities in comparison to their mouse orthologues by transgenic LacZ reporter assays, which was complemented by analysis of the endogenous gene expression in limb buds of both species. This analysis indicates that diversified activity of CARs and their mouse orthologues could be linked to the differential gene expression patterns in limb buds of both species

    Management Matters : Organizational Storytelling within the Anthroposophical Society in Sweden

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    The Anthroposophical Society, founded by the Austrian polymath Rudolf Steiner, came to Sweden in 1913, but for the generation of present-day Swedish Anthroposophists whose voices are heard in this study, the great flowering of the movement occurred in the second half of the twentieth century. The movement had by then expanded into a large milieu with many largely independent enterprises and institutions, from the formal organization itself, to various schools, farms, shops, medical facilities, etc., all based on interpretations of Steiner’s legacy. Since then, many members of the movement feel, there has been a decline. A movement of this size and complexity can be seen as a large organization with a corporate-like structure. Taking its point of departure in ideas from the vast field of organization studies, and specifically in the study of storytelling as part of the creation of a corporate culture where many voices and many perspectives co-exist, this study investigates how Anthroposophists in Sweden, both rank and-file members and some who served in leadership positions, tell the story of the putative Golden Age, decline, and projected future of Anthroposophy in Sweden. Twenty-eight interviews were collected, recurrent themes identified, and the plots of the various individual stories analyzed by means of a version of the actantial model developed by the semioticist Algirdas Greimas. The basic storyline, of which the interviewees’ individual stories constitute variations, is that the Golden Age, when charismatic leaders could draw crowds of enthusiastic young people and a vibrant Anthroposophical milieu was built up, came to an end with the demise of those leaders. The present, i.e., the time at which the interviews were conducted, is narratively framed as a period of sharp decline. The vistas for the future come across in most stories as quite bleak. An actantial analysis reveals that the past, an epoch that is on one hand held up as a shining example is on the other hand also described as a time characterized by innumerable problems and conflicts. Disagreement is rampant regarding the reasons for the current decline, and a vast number of problems are identified in the individual narratives. The future is for some interviewees impossible to speculate about, whereas others have specific suggestions for change. These suggestions, when held up against each other, show that there is no unified vision of what the necessary changes might be or who must bring them about. The interviewees agree that Anthroposophy plays a vital role as a spiritual path. When asked how they would describe Anthroposophy and what it more specifically can offer, answers diverge, but substantive descriptions of core concepts or practices are rarely alluded to. Rather, their explanations of what Anthroposophy is are in almost all cases metaphorical or negative, i.e., they represent Anthroposophy as elusive or undefinable. Interviewees can suggest that the lack of a clear Anthroposophical “brand” is a major reason for its current perceived crisis. An analysis of the ways in which Rudolf Steiner is portrayed in the interview material shows that there are a variety of descriptions of him rather than a unified representation of a charismatic leader that members can rally around. This, the study suggests, is because four different forms of charisma can be distinguished on theoretical grounds, and the particular form that permeates the narratives collected for this study does not readily support the dissemination of a centralized, dominant narrative.Antroposofiska SĂ€llskapet, grundat av österrikaren Rudolf Steiner, kom till Sverige redan i 1913, men för den generation av nutida svenska antroposofer vars röster hörs i denna studie intrĂ€ffade rörelsens stora blomstringstid först under nittonhundratalets andra hĂ€lft. Vid det laget hade rörelsen expanderat och blivit till en omfattande miljö med mĂ„nga stort sett oberoende institutioner och verksamheter, frĂ„n sjĂ€lva det Antroposofiska SĂ€llskapet i strikt mening till olika skolor, lantbruk, butiker, kliniker, osv., som alla byggde pĂ„ tolkningar av arvet efter Steiner. MĂ„nga medlemmar i rörelsen menar att det sedan dess har skett en nedgĂ„ng. En rörelse med den storlek och komplexitet som det rör sig om i det aktuella fallet kan betraktas som en organisation med en företagsliknande struktur. Denna studie tar dĂ€rför sin utgĂ„ngspunkt i ett organisationsteoretiskt perspektiv, i synnerhet i den gren av organisationsteorin som studerar berĂ€ttande som ett led i hur en organisationskultur med mĂ„nga samexisterande röster skapas. I det aktuella fallet handlar det om berĂ€ttelser som antroposofer i Sverige, bĂ„de vanliga medlemmar och personer i ledarstĂ€llning, framför om den blomstringstid de menar rörelsen en gĂ„ng hade, den nedgĂ„ng de sĂ€ger sig uppleva och den framtid de förestĂ€ller sig att antroposofin i Sverige kommer att möta. TjugoĂ„tta intervjuer genomfördes och de berĂ€ttelser som förmedlas i dessa intervjuer analyserades med hjĂ€lp av en variant av den aktantmodell som utvecklats av semiotikern Algirdas Greimas. Den grundlĂ€ggande handling man Ă„terfinner i intervjupersonernas olika berĂ€ttelser Ă€r att blomstringstiden var en guldĂ„lder dĂ„ karismatiska ledare kunde samla stora grupper av entusiastiska ungdomar och en levande antroposofisk miljö byggdes upp, men att denna guldĂ„lder upphörde nĂ€r ledarna gick ur tiden. Nuet, alltsĂ„ den tid dĂ„ intervjuerna genomfördes, beskrivs i berĂ€ttelserna som en tid av förfall. Framtidsutsikterna som mĂ„las upp i de flesta berĂ€ttelser Ă€r dystra. Aktantanalysen visar att berĂ€ttelserna om det förflutna bĂ„de beskriver denna tid i mycket positiva termer och nĂ€mner otaliga problem och konflikter. Nuets pĂ„stĂ„dda förfall Ă„terkommer i de flesta berĂ€ttelser, men Ă„sikterna gĂ„r vitt isĂ€r nĂ€r det gĂ€ller vad nutidens problem Ă€r och vad som orsakat dem. Framtiden beskrivs av vissa intervjupersoner som omöjlig att spekulera nĂ€rmare om, medan andra har specifika förslag till förĂ€ndringar. Sammantaget visar analysen att det saknas en enhetlig förestĂ€llning om vad som behöver göras för att lösa rörelsens problem och vem som ska ta ansvar för dessa förĂ€ndringar. Intervjupersonerna Ă€r eniga om att antroposofin spelar en viktig roll. FrĂ„gan hur de skulle beskriva antroposofin och vad den har att erbjuda besvaras pĂ„ olika sĂ€tt, men sĂ€llan i termer av konkreta beskrivningar av för antroposofin centrala förestĂ€llningar eller praktiker. Tendensen Ă€r snarare att svara i metaforiska eller negativa termer, alltsĂ„ genom att berĂ€tta att de menar att antroposofin inte gĂ„r att definiera. Samtidigt kan intervjupersonerna förklara att bristen pĂ„ en tydlig antroposofisk identitet Ă€r ett huvudskĂ€l till vad de ser som rörelsens nuvarande kris. En analys av de sĂ€tt pĂ„ vilka Rudolf Steiner beskrivs i intervjumaterialet visar att det ocksĂ„ finns en rad divergerande uppfattningar av honom snarare Ă€n en sammanhĂ„llen beskrivning av en karismatisk ledare som medlemmarna kan samlas kring. Studien konkluderar att karisma pĂ„ teoretiska grunder kan delas in i fyra olika typer, och att den specifika form av karisma som intervjuerna Ă„terspeglar inte harmonierar sĂ€rskilt vĂ€l med spridandet av en centralt utformad dominerande berĂ€ttelse

    Developing automated meta-research approaches in the preclinical Alzheimer's disease literature

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    Alzheimer’s disease is a devastating neurodegenerative disorder for which there is no cure. A crucial part of the drug development pipeline involves testing therapeutic interventions in animal disease models. However, promising findings in preclinical experiments have not translated into clinical trial success. Reproducibility has often been cited as a major issue affecting biomedical research, where experimental results in one laboratory cannot be replicated in another. By using meta-research (research on research) approaches such as systematic reviews, researchers aim to identify and summarise all available evidence relating to a specific research question. By conducting a meta-analysis, researchers can also combine the results from different experiments statistically to understand the overall effect of an intervention and to explore reasons for variations seen across different publications. Systematic reviews of the preclinical Alzheimer’s disease literature could inform decision making, encourage research improvement, and identify gaps in the literature to guide future research. However, due to the vast amount of potentially useful evidence from animal models of Alzheimer’s disease, it remains difficult to make sense of and utilise this data effectively. Systematic reviews are common practice within evidence based medicine, yet their application to preclinical research is often limited by the time and resources required. In this thesis, I develop, build-upon, and implement automated meta-research approaches to collect, curate, and evaluate the preclinical Alzheimer’s literature. I searched several biomedical databases to obtain all research relevant to Alzheimer’s disease. I developed a novel deduplication tool to automatically identify and remove duplicate publications identified across different databases with minimal human effort. I trained a crowd of reviewers to annotate a subset of the publications identified and used this data to train a machine learning algorithm to screen through the remaining publications for relevance. I developed text-mining tools to extract model, intervention, and treatment information from publications and I improved existing automated tools to extract reported measures to reduce the risk of bias. Using these tools, I created a categorised database of research in transgenic Alzheimer’s disease animal models and created a visual summary of this dataset on an interactive, openly accessible online platform. Using the techniques described, I also identified relevant publications within the categorised dataset to perform systematic reviews of two key outcomes of interest in transgenic Alzheimer’s disease models: (1) synaptic plasticity and transmission in hippocampal slices and (2) motor activity in the open field test. Over 400,000 publications were identified across biomedical research databases, with 230,203 unique publications. In a performance evaluation across different preclinical datasets, the automated deduplication tool I developed could identify over 97% of duplicate citations and a had an error rate similar to that of human performance. When evaluated on a test set of publications, the machine learning classifier trained to identify relevant research in transgenic models performed was highly sensitive (captured 96.5% of relevant publications) and excluded 87.8% of irrelevant publications. Tools to identify the model(s) and outcome measure(s) within the full-text of publications may reduce the burden on reviewers and were found to be more sensitive than searching only the title and abstract of citations. Automated tools to assess risk of bias reporting were highly sensitive and could have the potential to monitor research improvement over time. The final dataset of categorised Alzheimer’s disease research contained 22,375 publications which were then visualised in the interactive web application. Within the application, users can see how many publications report measures to reduce the risk of bias and how many have been classified as using each transgenic model, testing each intervention, and measuring each outcome. Users can also filter to obtain curated lists of relevant research, allowing them to perform systematic reviews at an accelerated pace with reduced effort required to search across databases, and a reduced number of publications to screen for relevance. Both systematic reviews and meta-analyses highlighted failures to report key methodological information within publications. Poor transparency of reporting limited the statistical power I had to understand the sources of between-study variation. However, some variables were found to explain a significant proportion of the heterogeneity. Transgenic animal model had a significant impact on results in both reviews. For certain open field test outcomes, wall colour of the open field arena and the reporting of measures to reduce the risk of bias were found to impact results. For in vitro electrophysiology experiments measuring synaptic plasticity, several electrophysiology parameters, including magnesium concentration of the recording solution, were found to explain a significant proportion of the heterogeneity. Automated meta-research approaches and curated web platforms summarising preclinical research could have the potential to accelerate the conduct of systematic reviews and maximise the potential of existing evidence to inform translation

    'Inventions and adventures': the work of the Stevenson engineering firm in Scotland, c. 1830 - c. 1890

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    This thesis examines the work of the nineteenth-century Stevenson civil engineering firm to argue that civil engineering should be approached geographically both because it takes place in and is shaped by particular spaces, but also because the result of such work reshapes space and the relationship between places. Geographers have extensively analysed the ways in which humans have worked to alter environments, but relatively little attention has been paid to engineering as a socially and geographically transformative process, to the technical questions and to the engineering professionals whose work brought about such change. This thesis analyses engineers as social and technical agents of environmental change, rather than viewing their role as the simple implementation of directives developed elsewhere and by others. It combines insights from the history and historical geography of science, environmental history and the history of technology to make a case for the relevance of an historical geography of engineering. The thesis explores these issues through the work of the Stevenson family. The Stevensons were an Edinburgh-based and internationally-renowned firm of engineers who specialised in the construction of coastal infrastructure. The start and end dates of the thesis indicate, broadly, the careers of David and Thomas Stevenson, who jointly managed the family firm under the name D. & T. Stevenson between 1850 and 1886. The empirical basis for this thesis draws upon the detailed analysis of the firm’s archival records: technical publications, project reports, diaries, correspondence, maps, plans and diagrams. The work of the Stevensons—their engineering epistemologies, practices, and professional identities— are examined through four diverse projects undertaken by the firm in the nineteenth century. These projects are: the training of new engineers; surveying and designing improvement works for the rivers Tay and Clyde; the implementation of a coastal sound-based fog signal network; and the failed attempt to expand Wick harbour through the construction of a breakwater. These projects highlight the range of activities undertaken by nineteenth-century engineers and illustrate the ‘making’ of engineers and the work they did by highlighting training and learning, surveying, maintenance, testing, evaluation, repair and the explanation of failure. With reference to these projects and by drawing upon relevant contextual material, the thesis examines the conceptualisation of geographical space and natural forces in engineering, the relationship between science and engineering, the nature of expertise and notions of engineering judgement, and the role of family, legacy and reputation in securing professional credibility and status. This approach challenges older historiographical traditions which portrayed engineers as individual geniuses. The thesis instead understands engineering to be a combination of specialist knowledge and tacit skill and situates engineers within their social and institutional networks of power and authority. In pointing out that some engineering works failed, the thesis challenges the tendency in histories of engineering works to focus on success. It makes the case for an historical geography of engineering as a way of understanding engineering as an activity, a status and as processes which changed human-environment relations
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