Introduction of non-linear elasticity models for characterization of shape and deformation statistics: application to contractility assessment of isolated adult cardiocytes

<p>Abstract</p> <p>Background</p> <p>We are exploring the viability of a novel approach to cardiocyte contractility assessment based on biomechanical properties of the cardiac cells, energy conservation principles, and information content measures. We define our measure of cell contraction as being the distance between the shapes of the contracting cell, assessed by the minimum total energy of the domain deformation (warping) of one cell shape into another. To guarantee a meaningful <it>vis-à-vis </it>correspondence between the two shapes, we employ both a data fidelity term and a regularization term. The data fidelity term is based on nonlinear features of the shapes while the regularization term enforces the compatibility between the shape deformations and that of a hyper-elastic material.</p> <p>Results</p> <p>We tested the proposed approach by assessing the contractile responses in isolated adult rat cardiocytes and contrasted these measurements against two different methods for contractility assessment in the literature. Our results show good qualitative and quantitative agreements with these methods as far as frequency, pacing, and overall behavior of the contractions are concerned.</p> <p>Conclusions</p> <p>We hypothesize that the proposed methodology, once appropriately developed and customized, can provide a framework for computational cardiac cell biomechanics that can be used to integrate both theory and experiment. For example, besides giving a good assessment of contractile response of the cardiocyte, since the excitation process of the cell is a closed system, this methodology can be employed in an attempt to infer statistically significant model parameters for the constitutive equations of the cardiocytes.</p

Variational Methods in Shape Space

This dissertation deals with the application of variational methods in spaces of geometric shapes. In particular, the treated topics include shape averaging, principal component analysis in shape space, computation of geodesic paths in shape space, as well as shape optimisation. Chapter 1 provides a brief overview over the employed models of shape space. Geometric shapes are identified with two- or three-dimensional, deformable objects. Deformations will be described via physical models; in particular, the objects will be interpreted as consisting of either a hyperelastic solid or a viscous liquid material. Furthermore, the description of shapes via phase fields or level sets is briefly introduced. Chapter 2 reviews different and related approaches to shape space modelling. References to related topics in image segmentation and registration are also provided. Finally, the relevant shape optimisation literature is introduced. Chapter 3 recapitulates the employed concepts from continuum mechanics and phase field modelling and states basic theoretical results needed for the later analysis. Chapter 4 addresses the computation of shape averages, based on a hyperelastic notion of shape dissimilarity: The dissimilarity between two shapes is measured as the minimum deformation energy required to deform the first into the second shape. A corresponding phase-field model is introduced, analysed, and finally implemented numerically via finite elements. A principal component analysis of shapes, which is consistent with the previously introduced average, is considered in Chapter 5. Elastic boundary stresses on the average shape are used as representatives of the input shapes in a linear vector space. On these linear representatives, a standard principal component analysis can be performed, where the employed covariance metric should be properly chosen to depend on the input shapes. Chapter 6 interprets shapes as belonging to objects made of a viscous liquid and correspondingly defines geodesic paths between shapes. The energy of a path is given as the total physical dissipation during the deformation of an object along the path. A rigid body motion invariant time discretisation is achieved by approximating the dissipation along a path segment by the deformation energy of a small solid deformation. The numerical implementation is based on level sets. Chapter 7 is concerned with the optimisation of the geometry and topology of solid structures that are subject to a mechanical load. Given the load configuration, the structure rigidity, its volume, and its surface area shall be optimally balanced. A phase field model is devised and analysed for this purpose. In this context, the use of nonlinear elasticity allows to detect buckling phenomena which would be ignored in linearised elasticity