First light for avian embryos: eggshell thickness and pigmentation mediate variation in development and UV exposure in wild bird eggs

Article first published online: 29 JUL 20141. The avian embryo's development is influenced by both the amount and the wavelength of the light that passes through the eggshell. Commercial poultry breeders use light of specific wavelengths to accelerate embryonic growth, yet the effects of the variably patterned eggshells of wild bird species on light transmission and embryonic development remain largely unexplored. 2. Here, we provide the first comparative phylogenetic analysis of light transmission, through a diverse range of bird eggshells (74 British breeding species), in relation to the eggshell's thickness, permeability, pigment concentration and surface reflectance spectrum (colour). 3. The percentage of light transmitted through the eggshell was measured in the spectral range 250–700 nm. Our quantitative analyses confirm anecdotal reports that eggshells filter the light of the externally coloured shell. Specifically, we detected a positive relationship between surface eggshell reflectance (‘brightness’) and the percentage of light transmitted through the eggshell, and this relationship was strongest at wavelengths in the human-visible blue-green region of the spectra (c. 435 nm). 4. We show that less light passes through thicker eggshells with greater total pigment concentrations. By contrast, permeability (measured as water vapour conductance) did not covary significantly with light transmission. Eggs of closed-nesting species let more light pass through, compared with open nesters. 5. We postulate that greater light transmission is required to assist embryonic development under low light exposure. Importantly, this result provides an ecological explanation for the repeated evolution of immaculate, white- or pale-coloured eggshells in species nesting in enclosed spaces. 6. Finally, we detected correlative support for the solar radiation hypothesis, in that eggshells of bird species with a longer incubation period let significantly less of the potentially harmful, ultraviolet (UV) light pass through the eggshell. In summary, we demonstrate suites of avian eggshell properties, including eggshell structure and pigmentation, which are consistent with an evolutionary pressure to both enhance and protect embryonic development.Golo Maurer, Steven J. Portugal, Mark E. Hauber, Ivan Mikšík, Douglas G. D. Russell and Phillip Casse

Eggshell pigment composition covaries with phylogeny but not with life history or with nesting ecology traits of British passerines

No single hypothesis is likely to explain the diversity in eggshell coloration and patterning across birds, suggesting that eggshell appearance is most likely to have evolved to fulfill many nonexclusive functions. By controlling for nonindependent phylogenetic associations between related species, we describe this diversity using museum eggshells of 71 British breeding passerine species to examine how eggshell pigment composition and concentrations vary with phylogeny and with life-history and nesting ecology traits. Across species, concentrations of biliverdin and protoporphyrin, the two main pigments found in eggshells, were strongly and positively correlated, and both pigments strongly covaried with phylogenetic relatedness. Controlling for phylogeny, cavity-nesting species laid eggs with lower protoporphyrin concentrations in the shell, while higher biliverdin concentrations were associated with thicker eggshells for species of all nest types. Overall, these relationships between eggshell pigment concentrations and the biology of passerines are similar to those previously found in nonpasserine eggs, and imply that phylogenetic dependence must be considered across the class in further explanations of the functional significance of avian eggshell coloration

Using eggshell membranes as a DNA source for population genetic research

In the context of population genetic research, a faster and less invasive method of DNA sampling would allow large-scale assessments of genetic diversity and genetic differentiation with the help of volunteer observers. The aim of this study was to investigate the usefulness of eggshell membranes as a DNA source for population genetic research, by addressing eggshell membrane DNA quality, degeneration and cross-contamination. To this end, a comparison was made with blood-derived DNA samples. We have demonstrated 100% successful DNA extraction from post-hatched Black-tailed Godwit (Limosa limosa) eggshell membranes as well as from blood samples. Using 11 microsatellite loci, DNA amplification success was 99.1% for eggshell membranes and 97.7% for blood samples. Genetic information within eggshell membrane DNA in comparison to blood DNA was not affected (F-ST = -0.01735, P = 0.999) by degeneration or possible cross-contamination. Furthermore, neither degeneration nor cross-contamination was apparent in total genotypic comparison of eggshell membrane DNA and blood sample DNA. Our research clearly illustrates that eggshell membranes can be used for population genetic research

Multiple dinosaur egg-shell occurrence in an Upper Cretaceous nesting site from Patagonia

The discovery of hundreds of megaloolithid-type egg-clutches (some including embryos of an indeterminate species of titanosaur sauropods) in several stratigraphical levels of the Late Cretaceous nesting site of Auca Mahuevo (Chiappe et al., 2005) unveiled important aspects of the reproductive behavior of sauropod dinosaurs and stimulated further work at other Patagonian dinosaur egg sites. In November of 2003, a join expedition of the National University of Comahue (Neuquén), the Museo Carmen Funes (Plaza Huincul) and the Museo de Lamarque (Lamarque) conducted fieldwork in several Late Cretaceous localities of Bajo Santa Rosa (center-north Río Negro Province, Argentina) bearing the remains of dinosaur eggs and other terrestrial vertebrates (figure 1). The main focus of this expedition was to assess the diversity of dinosaur eggs, to fine-tune the stratigraphy of the egg-bearing layers, and to document the spatial distribution of the egg-clutches. The overall results of this research were reported elsewhere (Salgado et al., 2007). In this contribution, we make focus in one of the fossil localities worked, Berthe IV, where the association of egg clutches likely belonging to different dinosaur species was detected (figure 1.2). Collected eggshells were observed with a binocular loupe ‘‘Stemi SV6 Zeiss’’, at magnifications of x1.0 and x3.2 (for macrocharacters). Microcharacters were observed in transverse thin section using a polarizing microscope (‘‘Zeiss Axioplan’’) at magnifications of x0.4 and x10. The eggshells were photographed using this polarizing microscope equipped with a digital camera. Prior to analysis, the eggshells were submitted to a process of cleaning using ultrasound (see Salgado et al., 2007 for further methodological details).Fil: Coria, Rodolfo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Provincia del Neuquen. Municipalidad de Plaza Huincul. Museo "Carmen Funes"; ArgentinaFil: Salgado, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación en Biodiversidad y Medioambiente; Argentina. Universidad Nacional del Comahue. Museo de Geologia y Paleontologia; ArgentinaFil: Chiappe, Luis M.. Los Angeles County Museum of Natural History. Dinosaur Institute; Estados Unido

Genetic variation in eggshell crystal size and orientation is large and these traits are correlated with shell thickness and are associated with eggshell matrix protein markers

The size and orientation of calcium carbonate crystals influence the structure and strength of the eggshells of chickens. In this study, estimates of heritability were found to be high (0.6) for crystal size and moderate (0.3) for crystal orientation. There was a strong positive correlation (0.65) for crystal size and orientation with the thickness of the shell and, in particular, with the thickness of the mammillary layer. Correlations with shell breaking strength were positive but with a high standard error. This was contrary to expectations, as in man-made materials smaller crystals would be stronger. We believe the results of this study support the hypothesis that the structural organization of shell, and in particular the mammillary layer, is influenced by crystal size and orientation, especially during the initial phase of calcification. Genetic associations for crystal measurements were observed between haplotype blocks or individual markers for a number of eggshell matrix proteins. Ovalbumin and ovotransferrin (LTF) markers for example were associated with crystal size, while ovocleidin-116 and ovocalyxin-32 (RARRES1) markers were associated with crystal orientation. The location of these proteins in the eggshell is consistent with different phases of the shell-formation process. In conclusion, the variability of crystal size, and to a lesser extent orientation, appears to have a large genetic component, and the formation of calcite crystals are intimately related to the ultrastructure of the eggshell. Moreover, this study also provides evidence that proteins in the shell influence the variability of crystal traits and, in turn, the shell’s thickness profile. The crystal measurements and/or the associated genetic markers may therefore prove to be useful in selection programs to improve eggshell quality

Use of chicken eggshell to improve dietary calcium intake in rural sub-Saharan Africa

Undernutrition resulting from inadequate access to high‐quality, nutritious food is a widespread issue in sub‐Saharan Africa impacting the health and survival of mothers and their children. Inadequate dietary intake leads to a deficiency in nutrients including calcium, required for growth and physiological functioning. This study investigated the potential of increasing dietary calcium intake by the addition of heat‐treated ground eggshell to locally prepared food. A mixed methods approach of literature review, Delphi expert survey and focus group discussions with women of childbearing age in rural Tanzania, were used to assess the practicality, safety, and acceptability of consumption of ground eggshell. Chicken eggshell has high calcium content (380 mg of calcium/gram) and bioavailability comparable to calcium carbonate (~39%) with 1 g sufficient to provide one half of a sub‐Saharan African adult female's dietary calcium needs. Salmonella was indicated as the most likely threat to human health through eggshell consumption. Experts agreed that eggshells boiled for 10 min when preparing hard‐boiled eggs with a further 20 min cooking of crushed eggshell in staple foods would eliminate identified egg‐associated pathogens. Five focus groups (n = 46) indicated eggshells were perceived as waste. However, there was an indication of general acceptance of the approach and a willingness to consider the incorporation of ground eggshells into their diets. Development of suitable communication methods are required to convey benefits and safe preparation methods. Ground eggshell could be a highly equitable method of increasing calcium intakes across rural sub‐Saharan Africa where calcium intake is low and village poultry ownership common

Preparation and characterisation of irradiated waste eggshells as oil adsorbent

Adsorption method had been developed by using natural organic adsorbent for the removal of oil because of its ability to bind the oil molecules into the surface of adsorbent. In this study, chicken eggshells waste was used and it undergoes irradiation process with four different amount of dose which was 0.5 kGy, 1.0 kGy, 1.5 kGy, and 2.0 kGy by using Gamma Cell Irradiator. Three equipment had been used for the characterization process which were the Scanning Electron Microscope (SEM), Energy Dispersive X-ray spectroscopy (EDX), and Fourier-Transform Infrared Spectroscopy (FTIR). The adsorption experiment was conducted to calculate the sorption efficiency by using different mass of samples. The result showed that irradiated chicken eggshells powder with 2.0 kGy amount of radiation dose has a best performance as oil adsorbent

Murray-Darling basin freshwater shells: riverine reservoir effect

We report carbon isotope measurements on pre-bomb museum samples of freshwater mussel shells collected alive from riverine locations in New South Wales, Australia. The calculated reservoir ages, ranging from -60 to +112 years, are much smaller than those for Australian marine shells and not considered significant for the radiocarbon dating of Late Pleistocene freshwater shells from the Murray-Darling Basin

Observations on Parental Care in the Family Aradidae (Heteroptera).

New observations of maternal care by Neuroctenus simplex and N. elongatus are reported

Soils of Seabee Hook, Cape Hallett, northern Victoria Land, Antarctica

The soils of the Seabee Hook area of Cape Hallett in northern Victoria Land, Antarctica, were mapped and characterized. Seabee Hook is a low-lying gravel spit of beach deposits built up by coastal currents carrying basalt material from nearby cliffs. Seabee Hook is the location of an Adélie penguin (Pygoscelis adeliae) colony which influences the soils with additions of guano, dead birds, eggshells and feathers. A soil-landscape model was developed and a soil association was identified between the soils formed on mounds (relict beach ridges) favoured by penguins for nests (Typic Haplorthel) and the soils in the areas between the mounds (Typic Haplorthel/Typic Aquorthel). Soils formed on the mounds inhabited by penguins contained guano in the upper 50 cm, overlying sub-rounded beach-deposited gravel and sand. Soils between mounds had a thin veneer (< 5 cm) of guano overlying basaltic gravelly sand similar to that in the lower parts of the mound soils. The soils had high concentrations of nitrogen, organic carbon, phosphorus, cadmium, zinc, copper, and increased electrical conductivity, within horizons influenced by penguin guano. Five buried penguin bones were collected from the base of soil profiles and radiocarbon dated. The dates indicate that Seabee Hook has been colonized by penguins for at least 1000 years