1 research outputs found
Discovery and Characterization of the Highly Active Fungal Immunomodulatory Protein Fip-vvo82
Volvaria
volvacea (Bull. ex Fr.)
Sing, an important edible and medicinal macro-fungus, has been used
to remedy various diseases for hundreds of years in East Asia. To
identify key proteins with the unique therapeutic activity in V. volvacea, we conducted a genomewide comparison
of V. volvacea protein families and
those of other edible fungi that lack therapeutic functions and identified
seven fungal immunomodulatory proteins (FIPs) in V.
volvacea. On the basis of the predicted physiological
and biochemical characteristics of the seven FIPs, the novel Fip-vvo82
was inferred to have high immunomodulatory activity; this was confirmed
by molecular and immunological experiments and further characterized
by modeling the three-dimensional structure and protein–protein
docking. This is the first study to show that V. volvacea has more than one FIP