3D Analysis of chromosome architecture: advantages and limitations with SEM

Three-dimensional mitotic plant chromosome architecture can be investigated with the highest resolution with scanning electron microscopy compared to other microscopic techniques at present. Specific chromatin staining techniques making use of simultaneous detection of back-scattered electrons and secondary electrons have provided conclusive information on the distribution of DNA and protein in barley chromosomes through mitosis. Applied to investigate the structural effects of different preparative procedures, these techniques were the groundwork for the ``dynamic matrix model{''} for chromosome condensation, which postulates an energy-dependent process of looping and bunching of chromatin coupled with attachment to a dynamic matrix of associated protein fibers. Data from SEM analysis shows basic higher order chromatin structures: chromomeres and matrix fibers. Visualization of nanogold-labeled phosphorylated histone H3 (ser10) with high resolution on chromomeres shows that functional modifications of chromatin can be located on structural elements in a 3D context. Copyright (C) 2005 S. Karger AG, Basel

Role based behavior analysis

Tese de mestrado, Segurança Informática, Universidade de Lisboa, Faculdade de Ciências, 2009Nos nossos dias, o sucesso de uma empresa depende da sua agilidade e capacidade de se adaptar a condições que se alteram rapidamente. Dois requisitos para esse sucesso são trabalhadores proactivos e uma infra-estrutura ágil de Tecnologias de Informacão/Sistemas de Informação (TI/SI) que os consiga suportar. No entanto, isto nem sempre sucede. Os requisitos dos utilizadores ao nível da rede podem nao ser completamente conhecidos, o que causa atrasos nas mudanças de local e reorganizações. Além disso, se não houver um conhecimento preciso dos requisitos, a infraestrutura de TI/SI poderá ser utilizada de forma ineficiente, com excessos em algumas áreas e deficiências noutras. Finalmente, incentivar a proactividade não implica acesso completo e sem restrições, uma vez que pode deixar os sistemas vulneráveis a ameaças externas e internas. O objectivo do trabalho descrito nesta tese é desenvolver um sistema que consiga caracterizar o comportamento dos utilizadores do ponto de vista da rede. Propomos uma arquitectura de sistema modular para extrair informação de fluxos de rede etiquetados. O processo é iniciado com a criação de perfis de utilizador a partir da sua informação de fluxos de rede. Depois, perfis com características semelhantes são agrupados automaticamente, originando perfis de grupo. Finalmente, os perfis individuais são comprados com os perfis de grupo, e os que diferem significativamente são marcados como anomalias para análise detalhada posterior. Considerando esta arquitectura, propomos um modelo para descrever o comportamento de rede dos utilizadores e dos grupos. Propomos ainda métodos de visualização que permitem inspeccionar rapidamente toda a informação contida no modelo. O sistema e modelo foram avaliados utilizando um conjunto de dados reais obtidos de um operador de telecomunicações. Os resultados confirmam que os grupos projectam com precisão comportamento semelhante. Além disso, as anomalias foram as esperadas, considerando a população subjacente. Com a informação que este sistema consegue extrair dos dados em bruto, as necessidades de rede dos utilizadores podem sem supridas mais eficazmente, os utilizadores suspeitos são assinalados para posterior análise, conferindo uma vantagem competitiva a qualquer empresa que use este sistema.In our days, the success of a corporation hinges on its agility and ability to adapt to fast changing conditions. Proactive workers and an agile IT/IS infrastructure that can support them is a requirement for this success. Unfortunately, this is not always the case. The user’s network requirements may not be fully understood, which slows down relocation and reorganization. Also, if there is no grasp on the real requirements, the IT/IS infrastructure may not be efficiently used, with waste in some areas and deficiencies in others. Finally, enabling proactivity does not mean full unrestricted access, since this may leave the systems vulnerable to outsider and insider threats. The purpose of the work described on this thesis is to develop a system that can characterize user network behavior. We propose a modular system architecture to extract information from tagged network flows. The system process begins by creating user profiles from their network flows’ information. Then, similar profiles are automatically grouped into clusters, creating role profiles. Finally, the individual profiles are compared against the roles, and the ones that differ significantly are flagged as anomalies for further inspection. Considering this architecture, we propose a model to describe user and role network behavior. We also propose visualization methods to quickly inspect all the information contained in the model. The system and model were evaluated using a real dataset from a large telecommunications operator. The results confirm that the roles accurately map similar behavior. The anomaly results were also expected, considering the underlying population. With the knowledge that the system can extract from the raw data, the users network needs can be better fulfilled, the anomalous users flagged for inspection, giving an edge in agility for any company that uses it

Robust visualization and discrimination of nanoparticles by interferometric imaging

Single-molecule and single-nanoparticle biosensors are a growing frontier in diagnostics. Digital biosensors are those which enumerate all specifically immobilized biomolecules or biological nanoparticles, and thereby achieve limits of detection usually beyond the reach of ensemble measurements. Here we review modern optical techniques for single nanoparticle detection and describe the single-particle interferometric reflectance imaging sensor (SP-IRIS). We present challenges associated with reliably detecting faint nanoparticles with SP-IRIS, and describe image acquisition processes and software modifications to address them. Specifically, we describe a image acquisition processing method for the discrimination and accurate counting of nanoparticles that greatly reduces both the number of false positives and false negatives. These engineering improvements are critical steps in the translation of SP-IRIS towards applications in medical diagnostics.R01 AI096159 - NIAID NIH HHSFirst author draf