Comparative genomics of Shiga toxin encoding bacteriophages

Background Stx bacteriophages are responsible for driving the dissemination of Stx toxin genes (stx) across their bacterial host range. Lysogens carrying Stx phages can cause severe, lifethreatening disease and Stx toxin is an integral virulence factor. The Stx-bacteriophage vB_EcoP-24B, commonly referred to as 24B, is capable of multiply infecting a single bacterial host cell at a high frequency, with secondary infection increasing the rate at which subsequent bacteriophage infections can occur. This is biologically unusual, therefore determining the genomic content and context of 24B compared to other lambdoid Stx phages is important to understanding the factors controlling this phenomenon and determining whether they occur in other Stx phages. Results The genome of the Stx2 encoding phage, 24B was sequenced and annotated. The genomic organisation and general features are similar to other sequenced Stx bacteriophages induced from Enterohaemorrhagic Escherichia coli (EHEC), however 24B possesses significant regions of heterogeneity, with implications for phage biology and behaviour. The 24B genome was compared to other sequenced Stx phages and the archetypal lambdoid phage, lambda, using the Circos genome comparison tool and a PCR-based multi-loci comparison system. Conclusions The data support the hypothesis that Stx phages are mosaic, and recombination events between the host, phages and their remnants within the same infected bacterial cell will continue to drive the evolution of Stx phage variants and the subsequent dissemination of shigatoxigenic potentia

Routing And Communication Path Mapping In VANETS

Vehicular ad-hoc network (VANET) has quickly become an important aspect of the intelligent transport system (ITS), which is a combination of information technology, and transport works to improve efficiency and safety through data gathering and dissemination. However, transmitting data over an ad-hoc network comes with several issues such as broadcast storms, hidden terminal problems and unreliability; these greatly reduce the efficiency of the network and hence the purpose for which it was developed. We therefore propose a system of utilising information gathered externally from the node or through the various layers of the network into the access layer of the ETSI communication stack for routing to improve the overall efficiency of data delivery, reduce hidden terminals and increase reliability. We divide route into segments and design a set of metric system to select a controlling node as well as procedure for data transfer. Furthermore we propose a system for faster data delivery based on priority of data and density of nodes from route information while developing a map to show the communication situation of an area. These metrics and algorithms will be simulated in further research using the NS-3 environment to demonstrate the effectiveness

Digital Communication Media: Resources and Distribution Channels on Forest and Land Fires Information in Riau Province of Indonesia During Covid-19 Pandemic Period

The control towards Indonesian forest and land fires in 2020 is deemed more complicated due to the Covid-19 outbreak. Its rapid dissemination encourages the central and regional government officials to focus the Covid-19 risk reduction through some policies, such as health protocol, social activity and large-scale meeting restrictions as well as independent regional quarantine, causing the controlling process towards the fires to be implemented by collaborating a direct method in disaster-vulnerable areas through digital communication media. The research is purposed to recognize the use of communication technology in controlling Indonesian forest and land fires during the Covid-19 pandemic period, comprising of qualitative research using data collection through interviews, observation, literature study, and online data searching. It results the significance of digital communication media for information resources as well as the channel of the fires’ information dissemination for internal task force and externals used by The Riau Province’s Task Force of Forest and Land Fire Control. The digital media are based on android system and website, such as SiPongi application, Info BMKG, LAPAN-Fire Hotspot, and Lancang Kuning Dashboard, while the others used for disseminating the information are Zoom meeting, Whatsapp, Instagram, Twitter, Facebook, and Youtube

Detailed plan for the COMET WP3 initial research activity - list of research projects and goals, participants and timing

Detailed plan for the COMET Work Package (WP) 3

Adoption of Clean Leather-Tanning Technologies in Mexico

In many developing countries, a host of financial, institutional, and political factors hamstring conventional environmental regulation. Given these constraints, a promising strategy for controlling pollution is to promote the voluntary adoption of clean technologies. Although this strategy has received considerable attention in policy circles, empirical research on the adoption of clean technologies in developing countries is limited. This paper presents historical background and original survey data on the adoption of five clean tanning technologies by a sample of 137 leather tanneries in León, Guanajuato, Mexico, a city where tanneries have serious environmental impacts and conventional environmental regulation has repeatedly failed to mitigate the problem. The analysis suggest that rather than top-down public-sector pressure and technical assistance, the key factor driving the adoption of clean tanning technologies in León is the bottom-up dissemination of information about the cost and quality benefits of the technologies.clean technology, leather tanning, developing country, Mexico

Candida albicans colonization and dissemination from the murine gastrointestinal tract : the influence of morphology and Th17 immunity

This article is protected by copyright. All rights reserved. This work was supported by the Wellcome Trust (086558, 080088, 102705), a Wellcome Trust Strategic Award (097377) and a studentship from the University of Aberdeen. D.K. was supported by grant 5R01AI083344 from the National Institute of Allergy and Infectious Diseases and by a Voelcker Young Investigator Award from the Max and Minnie Tomerlin Voelcker Fund.Peer reviewedPublisher PD

Structured approach to measure performance in construction research and development: performance measurement system development

Purpose of this paper: This study explore Performance Measurement applications within construction research and development (R\&D) to develop a structured approach to measure the performance of collaborative construction R\&D projects during its lifecycle from initiation, conceptualising, development and launch stages and at the project management. Design/methodology/approach: During the exploratory phase of the study, semi-structured interviews and a questionnaire survey were carried out to develop the performance measurement system (PMS). During the explanatory phase, the developed PMS was refined with a series of semi-structured interviews. The data was gathered from principal investigators, researchers and industrial partners involved in collaborative construction R\&D work. Findings: Main finding is the developed PMS to measure the performance of construction R\&D that consists of critical success factors, performance indicators and performance measures during the lifecycle of a R\&D project. Practical implications: The PMS developed through the study provides critical success factors, performance indicators and measures that need to be considered during the construction R\&D projects to measure the performance. Thus, through the implementation of the PMS, this study contributes to practice by assisting the performance improvement within construction R\&D projects. What is original / value of paper?: The developed PMS to measure the performance of construction R\&D project will provide value for those who are engaged in R\&D work (Principle investigators, researcher and industrial partners) to ensure the success of their projects

Mid-term report for the CORE Organic II funded project. “Innovative cropping Practices to increase soil health of organic fruit tree orchards” BIO-INCROP

Activities performed in the first part of BIO-INCROP project concern five of the eight main objectives fixed in the project proposal. They are: Evaluation of soil borne pest and pathogens involved in replant disease Role of rhizospheric bacterial and fungal communities in plant health Selection of naturally available resources to increase microbial diversity and biomass Compost and organic amendments Evaluation of biologically active formulates The document reports main research results and shows main items of dissemination activity performed in the first part of the project