New properties of a bioinspired pyridine benzimidazole compound as a novel differential staining agent for endoplasmic reticulum and Golgi apparatus in fluorescence live cell imaging

Indexación: Scopus.In this study, we explored new properties of the bioinspired pyridine benzimidazole compound B2 (2,4-di-tert-butyl-6-(3H-imidazo[4,5-c]pyridine-2-yl)phenol) regarding its potential use as a differential biomarker. For that, we performed 1D 1HNMR (TOCSY), UV-Vis absorption spectra in different organic solvents, voltammetry profile (including a scan-rate study), and TD-DFT calculations that including NBO analyses, to provide valuable information about B2 structure and luminescence. In our study, we found that the B2 structure is highly stable, where the presence of an intramolecular hydrogen bond (IHB) seems to have a crucial role in the stability of luminescence, and its emission can be assigned as fluorescence. In fact, we found that the relatively large Stokes Shift observed for B2 (around 175 nm) may be attributed to the stability of the B2 geometry and the strength of its IHB. On the other hand, we determined that B2 is biocompatible by cytotoxicity experiments in HeLa cells, an epithelial cell line. Furthermore, in cellular assays we found that B2 could be internalized by passive diffusion in absence of artificial permeabilization at short incubation times (15 min to 30 min). Fluorescence microscopy studies confirmed that B2 accumulates in the endoplasmic reticulum (ER) and Golgi apparatus, two organelles involved in the secretory pathway. Finally, we determined that B2 exhibited no noticeable blinking or bleaching after 1 h of continuous exposure. Thus, B2 provides a biocompatible, rapid, simple, and efficient way to fluorescently label particular organelles, producing similar results to that obtained with other well-established but more complex methods. © 2018 Llancalahuen, Fuentes, Carreño, Zúñiga, Páez-Hernández, Gacitúa, Polanco, Preite, Arratia-Pérez and Otero.https://www.frontiersin.org/articles/10.3389/fchem.2018.00345/ful

Integrated predictive genome-scale models to improve the metabolic re-engineering efficiency

One of the most common applications of metabolic circuits is to produce a desired chemical in a chassis organism, such as the Escherichia coli (E. coli), by importing heterologous genes encoding for the enzymes that participate in the biosynthetic pathway. Recently, an automated pipeline named RetroPath was developed to synthesise embedded metabolic circuits [1]. These circuits are to be embedded in E. coli for a wide range of applications such as regulating biomass productions, sensing specifc molecules, processing specific molecules, and releasing specific molecules. In this paper, we improve the efficiency of RetroPath via quadratic programming

Analysis of microbiological variation in PDO Vastedda della valle del Belìce cheese during the storage period

The PDO “Vastedda della valle del Belìce” is a Sicilian pasta filata sheep cheese, made from raw milk without starter addition. It is a small round cheese without rind, weighing about 500-700 g. It is cheese is marketed also out of Sicily to allow its marketing and to prolong its shelf-life. The aim of this work was to evaluate the variation of microbiological parameters during the shelf-life period. Then 162 Vastedda cheeses from 18 cheese-making processes in 7 farms have been analysed at different times of storage at 4°C (0, 15, 30, 45, 60, 75, 90, 105, 120 days). Coliforms and E. coli were detected into 7/18 cheese-making processes and their count decreased during the storage period. Enterococci were more resistant to high temperature achieved during the stretching and their were rather stable during storage period (105 cfu/g). The concentration of total Bacterial Count and Mesophilic Lactococci were around 107 cfu/g, while the concentration of Thermophilic Lactococci was higher (108 cfu/g) probably due to the stretching at 90°C. In conclusion, this study shows the good sanitary conditions of cheeses. Overall, the lactic flora was kept alive and vital at high concentration (> 107 cfu/g) until 120 days of storage

The Laspeyres bias in the Spanish consumer price index

The CPI compares the cost of acquiring a reference quantity vector at current and base prices. Such reference vector is the vector of mean quantities actually bought by a reference population, whose consumption patterns are investigated during a period t prior to the index base period 0. In this paper we show that unless one takes into account the price change between these two dates, the CPI ceases to be a proper statistical price index of the Laspeyres type. Among several negative consequences, the most important is that this omission produces a bias in the measurement of inflation which we call the `Laspeyres bias.' Using Spanish data, we estimate that, e.g., from 1992 to 1998, the size of the Laspeyres bias is -0.061 per cent per year, or about 6 per cent (in absolute terms) of the positive bias estimated by the Boskin commission for the U.S., which is equal to 1.1 per cent per year. The Laspeyres bias in shorter time periods reached -0.122, and -0.108 per cent per year in 1992, and 1997, respectivel

Microbial Cell Factories / From rumen to industry

The rumen is one of the most complicated and most fascinating microbial ecosystems in nature. A wide variety of microbial species, including bacteria, fungi and protozoa act together to bioconvert (ligno)cellulosic plant material into compounds, which can be taken up and metabolized by the ruminant. Thus, the rumen perfectly resembles a solution to a current industrial problem: the biorefinery, which aims at the bioconversion of lignocellulosic material into fuels and chemicals. We suggest to intensify the studies of the ruminal microbial ecosystem from an industrial microbiologists point of view in order to make use of this rich source of organisms and enzymes

Uso de processos químicos no controle do risco biológico de dejetos suínos.

bitstream/item/56395/1/publicacao-492.pdfProjeto/Plano de Ação: 02.07.06.007

Spartan Daily, May 23, 1961

Volume 48, Issue 126https://scholarworks.sjsu.edu/spartandaily/4180/thumbnail.jp

Spartan Daily, March 6, 2002

Volume 118, Issue 29https://scholarworks.sjsu.edu/spartandaily/10602/thumbnail.jp

Caracterização físico-químico e identificação de contaminantes microbiológicos e físicos da farinha de mandioca do grupo seca.

bitstream/item/27823/1/Doc219.pdfDisponível também on-line