Phylogeny of the celastraceae inferred from phytochrome B gene sequence and morphology

Phylogenetic relationships within Celastraceae were inferred using a simultaneous analysis of 61 morphological characters and 1123 base pairs of phytochrome B exon 1 from the nuclear genome. No gaps were interred, and the gene tree topology suggests that the primers were specific to a single locus that did not duplicate among the lineages sampled. This region of phytochrome B was most useful for examining relationships among closely related genera. Fifty-one species from 38 genera of Celastraceae were sampled. The Celastraceae sensu lato (including Hippocrateaceae) were resolved as a monophyletic group. Loesener\u27s subfamilies and tribes of Celastraceae were not supported. The Hippocrateaceae were resolved as a monophyletic group nested within a paraphyletic Celastraceae sensu stricto. Goupia was resolved as more closely related to Euphorbiaceae, Corynocarpaceae, and Linaceae than to Celastraceae. Plagiopteron (Flacourtiaceae) was resolved as the sister group of Hippocrateoideae. Brexia (Brexiaceae) was resolved as closely related to Elaeodendron and Pleurostylia. Canotia was resolved as the sister group of Acanthothamus within Celastraceae. Perrottetia and Mortonia were resolved as the sister group of the rest of the Celastraceae. Siphonodon was resolved as a derived member of Celastraceae. Maytenus was resolved as three disparate groups, suggesting that this large genus needs to be recircumscribed

Medicinal Plants in Pregnancy and Lactation: Perception of the Health Risk and Practical Educational Group in Araraquara, São Paulo State, Brazil

The use of medicinal plants among pregnant women and lactating is a common practice in diverse countries. However, many medicinal plants are contraindicated during pregnancy and lactating, due to various adverse effects, such as teratogenic, embryotoxic and abortive effects, exposing these women, their fetus and babies to health unknown risks. Thus, the purpose of this commentary, was to analyze the perception about the use of medicinal plants by pregnant women and lactating registered in the "baby on board" NGO, Araraquara, São Paulo state, Brazil, between 2010 at 2013. The group was constituted by 48 women, between the first and last trimester of pregnancy or whilst breastfeeding. Information was collected during group meetings by oral interview, using a questionnaire, as script. The nature of the study was a qualitative analysis. The results were based on reports about the use of medicinal plants by pregnant women during group meetings: use, indication of use, knowledge about risks. All participants received written and oral information about the study and they gave a written informed consent. The use of medicinal plants is a reality among pregnant and lactating women of the "baby on board" NGO. They reported that they feel that "natural" products are not harmful for their health. The primary information sources for the majority of women about medicinal plants during pregnancy are family, neighbors and herbalists. The plants most cited were: senna, chamomile, boldo, lemon balm, lemon grass. They were used mainly for: nausea, heartburn, indigestion, flatulence, intestinal and abdominal pain, anxiety, intestinal constipation and low milk production. The pregnant and lactating women lacked knowledge about the health risks of the use of medicinal plants and herbal medicines in pregnancy and lactation. They also reported difficulties in clarifying some questions about the use of medicinal plants with their doctors. The results of the present study showed that educative actions about the rational use of medicinal plants in pregnancy and breastfeeding could be part of the operating protocols to promote the maternal and child health programs in Araraquara. Thus, our results also suggest the importance of creating institutionalized places, to the implementation of continued education programs about rational use of medicinal plants in pregnancy and lactation. These targeted programs are not only for health professionals, but also for community members, pregnant women and breastfeeding. Our results pointed out the importance of guidance of doctors and healthcare professionals on the scientific studies about medicinal plants and herbal medicines and the risk/benefit of using herbs during pregnancy. Finally, it is noted the importance of the health professionals to inform women of childbearing on risks to their health, as well as on possibilities of utilization of herbs during fertile period, giving special attention to the potential risk of self-medication

Distribution of sex forms in the phanerogamic flora

In the plant kingdom, particularly in the phanerogamic flora, hermaphroditism is by far the most common, yet the number of other sex forms is not negligible. This study was undertaken with the view of ascertaining the relative proportions in which such sex forms occur. For this purpose Engler and Prantls "Natürliche Pflanzenfamilien" with all the Nachträge (which are complete up to 1912) have been used. The lists that follow are the results of this examination

INDEPENDENT ORIGINATION OF FLORAL ZYGOMORPHY, A PREDICTED ADAPTIVE RESPONSE TO POLLINATORS: DEVELOPMENTAL AND GENETIC MECHANISMS

Observations of floral development indicate that floral organ initiation in pentapetalous flowers more commonly results in a medially positioned abaxial petal (MAB) than in a medially positioned adaxial petal (MAD), where the medial plane is defined by the stem and the bract during early floral development. It was proposed that the dominant MAB petal initiation might impose a developmental constraint that leads to the evolution of limited patterns of floral zygomorphy in Asteridae, a family in which the floral zygomorphy develops along the medial plane and results in a central ventral (CV) petal in mature flowers. Here, I investigate whether the pattern of floral organ initiation may limit patterns of floral zygomorphy to evolve in pentapetalous angiosperms. I analyzed floral diagrams representing 405 species in 330 genera of pentapetalous angiosperms to reconstruct the evolution of floral organ initiation and the evolution of developmental processes that give rise to floral zygomorphy on a phylogenetic framework. Results indicate that MAB petal initiation is the most common; it occupies 86.2% of diversity and represents the ancestral state of floral organ initiation in pentapetalous angiosperms. The MAD petal initiation evolved 28 times independently from the ancestral MAB petal initiation. Among the 34 independent originations of floral zygomorphy, 76.5% of these clades represent MAB petal initiation, among which only 47% of the clades result a CV petal in mature flowers. The discrepancy is explained by the existence of developmental processes that result in floral zygomorphy along oblique planes of floral symmetry in addition to along the medial plane. Findings suggest that although the early floral organ initiation plays a constraining role to the evolution of patterns of floral zygomorphy, the constraint diverges along phylogenetically distantly related groups that allow the independent originations of floral zygomorphy through distinct development processes in pentapetalous angiosperms. In additional study, the butterfly-like flowers of Schizanthus are adapted to pollination by bees, hummingbirds, and moths. I investigated the genetic basis of the zygomorphic corolla, for which development is key to the explosive pollen release mechanism found in the species of Schizanthus adapted to bee pollinators. I examined differential gene expression profiles across the zygomorphic corolla of Schizanthus pinnatus, a bee-pollinated species, by analyzing RNA transcriptome sequencing (RNA- seq). Data indicated that CYC2 is not expressed in the zygomorphic corolla of Sc. pinnatus, suggesting CYC2 is not involved in the development of floral zygomorphy in Schizanthus (Solanaceae). The data also indicated that a number of genes are differentially expressed across the corolla

A study of Antimicrobial Activity, Acute Toxicity and Cytoprotective Effect of a Polyherbal Extract in a Rat Ethanol-HCl Gastric Ulcer Model.

The decoction of the aerial parts of Rhynchosia recinosa (A.Rich.) Bak. [Fabaceae] is used in combination with the stem barks of Ozoroa insignis Del. (Anacardiaceae), Maytenus senegalensis (Lam.) Excell. [Celastraceae] Entada abyssinica Steud. ex A.Rich [Fabaceae] and Lannea schimperi (Hochst.)Engl. [Anacardiaceae] as a traditional remedy for managing peptic ulcers. However, the safety and efficacy of this polyherbal preparation has not been evaluated. This study reports on the phytochemical profile and some biological activities of the individual plant extracts and a combination of extracts of the five plants. METHODS: A mixture of 80% ethanol extracts of R. recinosa, O. insignis, M. senegalensis, E. abyssinica and L. schimperi at doses of 100, 200, 400 and 800 mg/kg body wt were evaluated for ability to protect Sprague Dawley rats from gastric ulceration by an ethanol-HCl mixture. ytoprotective effect was assessed by comparison with a negative control group given 1% tween 80 in normal saline and a positive control group given 40 mg/kg body wt pantoprazole. The individual extracts and their combinations were also tested for antibacterial activity against four Gram negative bacteria; Escherichia coli (ATCC 25922), Salmonella typhi (NCTC 8385), Vibrio cholerae (clinical isolate), and Klebsiella pneumoniae (clinical isolate) using the microdilution method. In addition the extracts were evaluated for brine shrimp toxicity and acute toxicity in mice. Phytochemical tests were done using standard methods to determine the presence of tannins, saponins, steroids, cardiac glycosides, flavonoids, alkaloids and terpenoids in the individual plant extracts and in the mixed extract of the five plants. RESULTS: The combined ethanolic extracts of the 5 plants caused a dose-dependent protection against ethanol/HCl induced ulceration of rat gastric mucosa, reaching 81.7% mean protection as compared to 87.5% protection by 40 mg/kg body wt pantoprazole. Both the individual plant extracts and the mixed extracts of 5 plants exhibited weak to moderate antibacterial activity against four G-ve bacteria. Despite Ozoroa insignis being toxic to mice at doses above 1000 mg/kg body wt, the other plant extracts and the combined extract of the 5 plants were tolerated by mice up to 5000 mg/kg body wt. The brine shrimp test results showed the same pattern of toxicity with Ozoroa insignis being the most toxic (LC50 = 10.63 mug/ml). Phytochemical tests showed that the combined extract of the five plants contained tannins, saponins, steroids, cardiac glycosides, flavonoids and terpenoids. Flavonoids, tannins and terpenoids are known to have antioxidant activity. CONCLUSION: The combined extract of the five plants exhibited a dose-dependent protective activity in the rat ethanol-HCl gastric ulcer model. The extracts also exhibited weak antibacterial activity against four Gram negative bacteria and low acute toxicity in mice and brine shrimps. Although the results support claims by traditional healers who use a decoction of the five plants for treatment of peptic ulcers, more models of gastric ulceration and proper animal toxicity studies are needed to validate possible clinical use of the polyherbal extract. It is also evident that the doses of the crude extracts showing protection of the gastric mucosa are too large for realistic translation to direct clinical application, but further studies using bioassay guided fractionation are important to either identify more practical fractions or active compound/s

Tree Species Composition and Forest Stratification along the Gradients in the Dry Deciduous Forests of Godavari Valley, Telangana, India

It is important to understand the tree species composition, abundance, species diversity and stratification in tropical dry deciduous forests that are under threat. A quadrat study was attempted in the dry deciduous forests along the ecological gradients in the Godavari Valley of northern Telangana, India. The study records the presence of 110 flowering plant taxa belonging to 82 genera and 37 families in 120 sampled plots, and there was enumeration of 15,192 individuals of ≥10 cm girth at breast height. Tectona grandis (teak) is the principal forest cover component in the region, which often formed pure stands in Adilabad and, to some extent, in Nizamabad districts. Further down to the Warangal district, teak was gradually replaced by Terminalia alata. Twenty tree species were found dominant at one place to the other, and the top 10 dominant taxa have shared nearly 41% of the total density of the forest cover. The tree relative density ranged from 0.007% to 20.84%. The values of Importance Value Index were between 0.245 (12 spp. including some exotics) and 32.6 (teak). These baseline data help to know the change detection along the gradients in the tropical forest ecosystem of a major river valley in the region and the drivers of change

Celastraceae

Subarbustos, arbustos o árboles de hojas simples, alternas u opuestas, brevemente pecioladas, con estípulas apareadas, pequeñas, caducas, lámina foliar entera o dentada. Inflorescencias cimosas en dicasios o monocasios simples o agrupados en racimos, hasta flores solitarias. Flores perfectas o imperfectas, actinomorfas, pequeñas, 4-5-meras. Sépalos persistentes, imbricados, soldados en la base. Pétalos caducos, imbricados, alternisépalos, amarillo verdosos, raro ausentes. Estambres 4-5 (-10), alternipétalos, libres entre sí, filamentos insertos debajo del disco, anteras bitecas con 2 lóculos cada uno, basifijas, de dehiscencia longitudinal introrsa o latrorsa. Disco carnoso rodeando el ovario parcial o totalmente, circular o pentago-nal. Ovario súpero, 2-5-carpelar, 2-5-locular, cada lóculo 2-ovulado, rudimentario en las flores estaminadas. Ovulos anátropos, erguidos, bitegumentados. Estilo simple, cilindro-cónico. Estigma capitado o 2-lobado, raro bífido, atrofiado en las flores estaminadas. Fruto cápsula 2-3-valvada o indehiscente, samaroide o drupáceo. Semi-llas con o sin arilo, endospermadas, con embrión recto de cotiledones aplanados

Eocene–early Oligocene climate and vegetation change in southern China: Evidence from the Maoming Basin

Although the Eocene-Oligocene climate transition marks a critical point in the development of the ‘icehouse’ global climate of the present little is known about this important change in the terrestrial realm at low latitudes. Our palynological study of the Shangcun Formation shows it to be early Oligocene in age: palyno-assemblages in the lower part of the formation indicate a cool interval dominated by conifer pollen in the earliest Oligocene followed by a warmer regime in the second half of the early Oligocene. To quantify middle Eocene to late early Oligocene climate conditions at low (~ 20°N) palaeolatitudes in southern Asia several thousand leaf fossil specimens from the Maoming Basin, southern China, were subjected to a multivariate (CLAMP) analysis of leaf form. For terrestrial palaeoclimate comparisons to be valid the palaeoaltitude at which the proxy data are obtained must be known. We find that leaves preserved in the Youganwo (middle Eocene), Huangniuling (late Eocene) and Shangcun (early Oligocene) formations were likely to have been deposited well above sea level at different palaeoelevations. In the Youganwo Formation fine-grained sediments were deposited at an altitude of ~ 1.5 km, after which the basin dropped to ~ 0.5 km by the time the upper Huangniuling sediments were deposited. The basin floor then rose again by 0.5 km reaching an altitude of approximately 1 km in which the Shangcun Formation fine-grained sediments were accumulated. Within the context of these elevation changes the prevailing climates experienced by the Youganwo, Lower Huangniuling, Upper Huangniuling and Shangcun fossil floras were humid subtropical with hot summers and warm winters, but witnessed a progressive increase in rainfall seasonality. By the early Oligocene rainfall seasonality was similar to that of the modern monsoonal climate of Guangdong Province, southern China. All floras show leaf physiognomic spectra most similar to those growing under the influence of the modern Indonesia-Australia Monsoon, but with no evidence of any adaptation to today's South or East Asia Monsoon regimes. The Upper Huangniuling Flora, rich in dipterocarp plant megafossils, grew in the warmest conditions with the highest cold month mean temperature and at the lowest altitude

МЕТОД АРХИТЕКТУРНЫХ МОДЕЛЕЙ В АНАЛИЗЕ ПОБЕГОВЫХ СИСТЕМ ПРЕДСТАВИТЕЛЕЙ СЕМЕЙСТВА CELASTRACEAE R.Br.

Работа посвящена анализу структурных и ритмологических особенностей побеговых систем (вегетативных и репродуктивных) представителей семейства Celastraceae с помощью концепции архитектурных моделей. Большинство бересклетовых относятся к группе моделей немодулярных, у которых система побегов формируется на основании только единиц морфогенеза при моноподиальном нарастании оси. Рост при этом может быть ритмичным (модель Rauh), либо непрерывным (модель Attims). Некоторых лиановидных представителей семейства с верхушечными соцветиями (Celastrus, Tripterygium) можно охарактеризовать моделями Champagnat и Mangenot

Taxonomic and phylogenetic census of the Celastrales: A synthetic review

A comprehensive assessment of the taxonomic and phylogenetic status of the celeastralean plexus is presented. An attempt has been made to review synthetically based on the data from different disciplines divulged by earlier authors and from present author’s study on the alliance. The taxonomic literature indicated that the Celeastrales (sensu lato) are a loose-knit assemblage. The tribal, subfamilial, familial and even ordinal boundaries are uncertain and even criss-cross each other. It appeared that the alliance can be grouped under two taxonomic entities viz., the Celastrales and the Rhamnales which appear evolved convergently