Long-term observations of increasing snow cover in the western Cairngorms

As part of the UK Environmental Change Network (ECN) long-term monitoring, an automatic repeat-photography camera was installed to record changes in landscape phenology in the Allt a'Mharcaidh catchment, Cairngorms National Park, Scotland. For 13 consecutive winters between 2002 and 2015, the date for the onset of continuous winter snow cover, and subsequent melt, was recorded on slopes of north and north-easterly aspect at altitudes between 450m and 1111m amsl. Results show that the period of time during which snow is continuously present in the catchment has increased significantly by 81 (±21.01) days over the 13-year period, and that this is largely driven by a significantly later melt date, rather than earlier onset of winter snow cover

The extent of NGC 6822 revealed by its C stars population

Using the CFH12K camera, we apply the four band photometric technique to identify 904 carbon stars in an area 28' x 42' centered on NGC 6822. A few C stars, outside of this area were also discovered with the Las Campanas Swope Telescope. The NGC 6822 C star population has an average I of 19.26 mag leading to an average absolute I magnitude of -4.70 mag, a value essentially identical to the mean magnitude obtained for the C stars in IC 1613. Contrary to stars highlighting the optical image of NGC 6822, C stars are seen at large radial distances and trace a huge slightly elliptical halo which do not coincide with the huge HI cloud surrounding NGC6822. The previously unknown stellar component of NGC 6822 has a exponential scale length of 3.0' +/- 0.1' and can be traced to five scale lengths. The C/M ratio of NGC 6822 is evaluated to br 1.0 +/- 0.2.Comment: accepted, to be published in A

A new approach to the solution of Maxwell's equations for low frequency and high-resolution biomedical problems

High spatial resolution studies of the interaction of the human body with electromagnetic waves of low frequency presents a difficult computational problem. As these studies typically require at least $10^4$ points per wavelength, a huge number of time steps would be needed to be able to use the finite difference time domain method (FDTD). In this paper, a new technique is described, which allows the FDTD method to be efficiently applied over a very large frequency range, including low frequencies. In the method, no alterations to the properties of either the source or the transmission media are required. The method is essentially frequency independent and has been verified against analytical solutions within the frequency range 50 Hertz to 1 Gigahertz. As an example of the lower frequency range, the method has been applied to the simulation of electromagnetic field behavior in the human body exposed to the pulsed magnetic field gradients of a magnetic resonance image (MRI) system

Signaling of the p21-activated kinase (PAK1) coordinates insulin-stimulated actin remodeling and glucose uptake in skeletal muscle cells

Skeletal muscle accounts for ~80% of postprandial glucose clearance, and skeletal muscle glucose clearance is crucial for maintaining insulin sensitivity and euglycemia. Insulin-stimulated glucose clearance/uptake entails recruitment of glucose transporter 4 (GLUT4) to the plasma membrane (PM) in a process that requires cortical F-actin remodeling; this process is dysregulated in Type 2 Diabetes. Recent studies have implicated PAK1 as a required element in GLUT4 recruitment in mouse skeletal muscle in vivo, although its underlying mechanism of action and requirement in glucose uptake remains undetermined. Toward this, we have employed the PAK1 inhibitor, IPA3, in studies using L6-GLUT4-myc muscle cells. IPA3 fully ablated insulin-stimulated GLUT4 translocation to the PM, corroborating the observation of ablated insulin-stimulated GLUT4 accumulation in the PM of skeletal muscle from PAK1−/− knockout mice. IPA3-treatment also abolished insulin-stimulated glucose uptake into skeletal myotubes. Mechanistically, live-cell imaging of myoblasts expressing the F-actin biosensor LifeAct-GFP treated with IPA3 showed blunting of the normal insulin-induced cortical actin remodeling. This blunting was underpinned by a loss of normal insulin-stimulated cofilin dephosphorylation in IPA3-treated myoblasts. These findings expand upon the existing model of actin remodeling in glucose uptake, by placing insulin-stimulated PAK1 signaling as a required upstream step to facilitate actin remodeling and subsequent cofilin dephosphorylation. Active, dephosphorylated cofilin then provides the G-actin substrate for continued F-actin remodeling to facilitate GLUT4 vesicle translocation for glucose uptake into the skeletal muscle cell

Electrophysiology of Sodium Receptors in Taste Cells

Sodium intake is important to maintain proper osmolarity and volume of extracellular fluid in vertebrates. The ability to find sources of sodium ions for managing electrolyte homeostasis relies on the activity of the taste system to sense salt. Several studies have been performed to understand the mechanisms underlying Na+ reception in taste cells, the peripheral detectors for food chemicals. It is now generally accepted that Na+ interacts with specific ion channels in taste cell membrane, called sodium receptors. As ion channels, these proteins mediate transmembrane ion fluxes (that is, electrical currents) during their operation. Thus, a lot of information on the functional properties of sodium receptors has been obtained by using electrophysiological techniques. Here, I review our current knowledge on the biophysical and physiological features of these receptors obtained by applying the patch-clamp recording techniques to single taste cells

Cyclic-AMP regulates postnatal development of neural and behavioral responses to NaCl in rats

During postnatal development rats demonstrate an age-dependent increase in NaCl chorda tympani (CT) responses and the number of functional apical amiloride-sensitive epithelial Na+channels (ENaCs) in salt sensing fungiform (FF) taste receptor cells (TRCs). Currently, the intracellular signals that regulate the postnatal development of salt taste have not been identified. We investigated the effect of cAMP, a downstream signal for arginine vasopressin (AVP) action, on the postnatal development of NaCl responses in 19–23 day old rats. ENaC-dependent NaCl CT responses were monitored after lingual application of 8-chlorophenylthio-cAMP (8-CPT-cAMP) under open-circuit conditions and under ±60 mV lingual voltage clamp. Behavioral responses were tested using 2 bottle/24h NaCl preference tests. The effect of [deamino-Cys1, D-Arg8]-vasopressin (dDAVP, a specific V2R agonist) was investigated on ENaC subunit trafficking in rat FF TRCs and on cAMP generation in cultured adult human FF taste cells (HBO cells). Our results show that in 19–23 day old rats, the ENaC-dependent maximum NaCl CT response was a saturating sigmoidal function of 8-CPT-cAMP concentration. 8-CPT-cAMP increased the voltage-sensitivity of the NaCl CT response and the apical Na+ response conductance. Intravenous injections of dDAVP increased ENaC expression and γ-ENaC trafficking from cytosolic compartment to the apical compartment in rat FF TRCs. In HBO cells dDAVP increased intracellular cAMP and cAMP increased trafficking of γ- and δ-ENaC from cytosolic compartment to the apical compartment 10 min post-cAMP treatment. Control 19–23 day old rats were indifferent to NaCl, but showed clear preference for appetitive NaCl concentrations after 8-CPT-cAMP treatment. Relative to adult rats, 14 day old rats demonstrated significantly less V2R antibody binding in circumvallate TRCs. We conclude that an age-dependent increase in V2R expression produces an AVP-induced incremental increase in cAMP that modulates the postnatal increase in TRC ENaC and the neural and behavioral responses to NaCl