FSD-C10, a Fasudil derivative, promotes neuroregeneration through indirect and direct mechanisms.

FSD-C10, a Fasudil derivative, was shown to reduce severity of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS), through the modulation of the immune response and induction of neuroprotective molecules in the central nervous system (CNS). However, whether FSD-C10 can promote neuroregeneration remains unknown. In this study, we further analyzed the effect of FSD-C10 on neuroprotection and remyelination. FSD-C10-treated mice showed a longer, thicker and more intense MAP2 and synaptophysin positive signal in the CNS, with significantly fewer CD4(+) T cells, macrophages and microglia. Importantly, the CNS of FSD-C10-treated mice showed a shift of activated macrophages/microglia from the type 1 to type 2 status, elevated numbers of oligodendrocyte precursor cells (OPCs) and oligodendrocytes, and increased levels of neurotrophic factors NT-3, GDNF and BDNF. FSD-C10-treated microglia significantly inhibited Th1/Th17 cell differentiation and increased the number of IL-10(+) CD4(+) T cells, and the conditioned medium from FSD-C10-treated microglia promoted OPC survival and oligodendrocyte maturation. Addition of FSD-C10 directly promoted remyelination in a chemical-induced demyelination model on organotypic slice culture, in a BDNF-dependent manner. Together, these findings demonstrate that FSD-C10 promotes neural repair through mechanisms that involved both immunomodulation and induction of neurotrophic factors

Structure and mechanical properties of artificial protein hydrogels assembled through aggregation of leucine zipper peptide domains

Artificial protein hydrogels made from a triblock protein (designated AC10A, where A is an acidic zipper domain and C10 comprises 10 repeats of the nonapeptide sequence exhibit normalized plateau storage moduli (G/nkT) less than 0.13 at all concentrations, pH values, and ionic strengths examined. These gels are surprisingly soft due to loop formation at the expense of bridges between physical junctions. Molecular-level evidence of loop formation is provided by strong fluorescence energy transfer (FRET) between distinct chromophores placed at the C- and N-termini of labelled chains diluted in an excess of unlabelled chains. The tendency to form loops originates from the compact size of the random coil midblock (mean RH(C10) 20 Å, determined from quasi-elastic light scattering of C10), and is facilitated by the ability of the leucine zipper domains to form antiparallel aggregates. Although the aggregation number of the leucine zipper domains is small (tetrameric, determined from multi-angle static light scattering of AC10 diblock), the average center-to-center distance between aggregates is roughly 1.5 times the average end-to-end distance of the C10 domain in a 7% w/v network. To avoid stretching the C10 domain, the chains tend to form loops. Changes in pH or ionic strength that expand the polyelectrolyte midblock favor bridging, leading to greater G as long as leucine zipper endblocks do not dissociate. Understanding of the network structure provided successful design strategies to increase the rigidity of these hydrogels. In contrast to intuitive design concepts for rubber and gel materials, it was shown that increasing either the length or the charge density of the midblock increases rigidity, because fewer chains are wasted in loop formation

Far infrared supplement: Catalog of infrared observations

The Far Infrared Supplement: catalog of infrared observations summarizes all infrared astronomical observations at far infrared wavelengths published in the scientific literature between 1965 and 1982. The Supplement list contains 25% of the observations in the full catalog of infrared observations (C10), and essentially eliminates most visible stars from the listings. The Supplement is more compact than the main Catalog (it does not contain the bibliography and position index of the C10), and is intended for easy reference during astronomical observations

Calibration and accuracy assessment of Leica ScanStation C10 terrestrial laser scanner

Requirement of high accuracy data in surveying applications has made calibration procedure a standard routine for all surveying instruments. This is due to the assumption that all observed data are impaired with errors. Thus, this routine is also applicable to terrestrial laser scanner (TLS) to make it available for surveying purposes. There are two calibration approaches: (1) component, and (2) system calibration. With the intention to specifically identify the errors and accuracy of the Leica ScanStation C10 scanner, this study investigates component calibration. Three components of calibration were performed to identify the constant, scale error, accuracy of angular measurement and the effect of angular resolution for distance measurement. The first calibration has been processed using closed least square solutions and has yielded the values of constant (1.2 mm) and scale error (1.000008879). Using variance ratio test (F-Test), angles observation (horizontal and vertical) for Leica C10 scanner and Leica TM5100A theodolite have shown significance difference. This is because the accuracy of both sensors are not similar and these differences are 0.01 and 0.0075º for horizontal and vertical measurements, respectively. Investigation on the resolution setting for Leica C10 scanner has highlighted the drawback of the tilt-and-turn target. Using the highest resolution, Leica Cyclone software only able to recognize the tilt-and-turn target up to 10 m distance compare to 200 m for the black and white target

Synthesis and Evaluation of Saccharide-Based Aliphatic and Aromatic Esters as Antimicrobial and Antibiofilm Agents

A small library of sugar-based (i.e., glucose, mannose and lactose) monoesters containing hydrophobic aliphatic or aromatic tails were synthesized and tested. The antimicrobial activity of the compounds against a target panel of Gram-positive, Gram-negative and fungi was assessed. Based on this preliminary screening, the antibiofilm activity of the most promising molecules was evaluated at different development times of selected food-borne pathogens (E. coli, L. monocytogenes, S. aureus, S. enteritidis). The antibiofilm activity during biofilm formation resulted in the following: mannose C10 > lactose biphenylacetate > glucose C10 > lactose C10. Among them, mannose C10 and lactose biphenylacetate showed an inhibition for E. coli 97% and 92%, respectively. At MICs values, no toxicity was observed on Caco-2 cell line for all the examined compounds. Overall, based on these results, all the sugar-based monoesters showed an interesting profile as safe antimicrobial agents. In particular, mannose C10 and lactose biphenylacetate are the most promising as possible biocompatible and safe preservatives for pharmaceutical and food applications

Breast tumor diagnosis in digital mammograms

Breast cancer has been classified as the most common cancer in most part of the world [1]. Breast cancer is caused by the growth of the abnormal cells in the breast. Breast cancer not only develops in women but also on men. However, the incidents of breast cancer in women are more common than men. Breast cancer is dangerous and may take away one’s life if there is no early detection and treatment are not done to remove the cancer cell present in the breast. Although the prevention methods for breast cancer may be unclear, it is found out that the earlier the detection and treatment conducted to the patients, the higher the survivability of the patients. Digital mammography is a specific type of breast imaging that uses low-dose x-rays to detect cancer early especially before women experience any symptoms [2]. The early signs of breast cancer can be detected in mammograms. Hence, digital mammograms have been classified as one of the best methods to detect breast cancer. In the studies [2] has shown that digital mammograms produce a better result than film mammograms in a group of young women, premenopausal and perimenopausal women, and women with dense breasts. 335 women were found to be infected with breast cancer in the test. However, there is also a limitation present in digital mammograms. High breast density can affect the performance of diagnosis in digital mammography as it increases the difficulty in finding abnormalities in a mammogram. Digital mammograms are only able to yield the best accuracy in the result for the women who are under the age of 50 and absent from menopause or undergoes menopause in a period of less than one year

Ambient Atmospheric Hydrocarbon Content as Determined by Gas Chromatographic Techniques from Rural Tidewater Virginia in Late Spring 1974

In an attempt to ascertain the naturally generated hydrocarbon contribution to the air quality of the Hampton Roads region of Tidewater Virginia, a series of 27 air samples was obtained in two rural locations during late spring of 1974. These samples were analyzed for their hydrocarbon content (carbon number range C5 to C10) using gas chromatographic techniques. The thirty different hydrocarbon species were identified and monitored in the experiment. Preliminary analysis of the data indicates an average concentration of 397 parts per billion by weight (carbon) for the total non-methane hydrocarbon loading for C5 to C10 during the experiment. This value exceeds the National Primary Air Quality Standards as set by the Environmental Protection Agency