935,936 research outputs found
Search for double charmonium decays of the P-wave spin-triplet bottomonium states
Using a sample of 158 million events collected with the Belle
detector, we search for the first time for double charmonium decays of the
-wave spin-triplet bottomonium states (,
\chi_{bJ} \to \jpsi \jpsi, \jpsi \psp, \psp \psp for J=0, 1, and 2). No
significant signal is observed in the double charmonium mass
spectra, and we obtain the following upper limits, \BR(\chi_{bJ} \to \jpsi
\jpsi)<7.1\times 10^{-5}, , ,
\BR(\chi_{bJ} \to \jpsi \psp)<1.2\times 10^{-4}, ,
, \BR(\chi_{bJ} \to \psp \psp)<3.1\times 10^{-5},
, for J=0, 1, and 2, respectively, at
the 90% confidence level. These limits are significantly lower than the central
values (with uncertainties of 50% to 70%) predicted using the light cone
formalism but are consistent with calculations using the NRQCD factorization
approach.Comment: 7 pages, 4 figures, 1 tabl
Chaperone-mediated native folding of a β-scorpion toxin in the periplasm of E.coli
Background: Animal neurotoxin peptides are valuable probes for investigating ion channel structure/function relationships and represent lead compounds for novel therapeutics and insecticides. However, misfolding and aggregation are common outcomes when toxins containing multiple disulfides are expressed in bacteria.
Methods: The ß-scorpion peptide toxin Bj-xtrIT from Hottentotta judaica and four chaperone enzymes (DsbA, DsbC, SurA and FkpA) were co-secreted into the oxidizing environment of the E.coli periplasm. Expressed Bj-xtrIT was purified and analyzed by HPLC and FPLC chromatography. Its thermostability was assessed using synchrotron radiation circular dichroism spectroscopy and its crystal structure was determined.
Results: Western blot analysis showed that robust expression was only achieved when cells co-expressed the chaperones. The purified samples were homogenous and monodisperse and the protein was thermostable. The crystal structure of the recombinant toxin confirmed that it adopts the native disulfide connectivity and fold.
Conclusions: The chaperones enabled correct folding of the four-disulfide-bridged Bj-xtrIT toxin. There was no apparent sub-population of misfolded Bj-xtrIT, which attests to the effectiveness of this expression method.
General Significance: We report the first example of a disulfide-linked scorpion toxin natively folded during bacterial expression. This method eliminates downstream processing steps such as oxidative refolding or cleavage of a fusion-carrier and therefore enables efficient production of insecticidal Bj-xtrIT. Periplasmic chaperone activity may produce native folding of other extensively disulfide-reticulated proteins including animal neurotoxins. This work is therefore relevant to venomics and studies of a wide range of channels and receptors
Impact of Deadlift and Power Clean on Vertical and Broad Jump Performance
A transfer between weightlifting and jumps is based on principles of increased demands being placed upon the muscular system while performing similar movement patterns. The purpose of the study was to investigate the impact of power-clean (PC) and deadlift (DL) interventions on vertical-jump (VJ) and broad-jump (BJ) performance in college-aged males. The hypothesis stated PC intervention would show greater improvements in BJ and VJ than DL intervention. The null hypothesis stated no difference between DL and PC groups would be found in affecting VJ and BJ performance. Six males who were not D-I athletes and were experienced with required movements were recruited for the study. Participants were randomly assigned to DL intervention, PC intervention, or control group. ORPYX shoe pods were placed in participants shoes to measure force produced in jumps and lifts. All participants performed pre-intervention max VJ and BJ testing. Jump testing was followed by max PC and DL testing for respected groups. Participants in DL and PC interventions performed a training protocol three days a week for six-weeks. Post-intervention, subjects were re-evaluated in jumps and lifts. Data was analyzed through ORPYX and transferred to Excel for further analysis. Means and standard deviations for force, jumps, and lifts were calculated and analyzed through SPSS. A one-way ANOVA was used to analyze data. Improvements occurred, but no statistically significant difference was observed (p \u3c .05). The null hypothesis was accepted; no significant differences were found between DL and PC in affecting VJ and BJ performance
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