Parental Selection for Productivity Improvement in Indian Tropical Tasar Silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae)

Global demand for Vanya silks in general and tasar silk in particular, call-for sustainable utilization of the country’s seri-biodiversity potential. Viability and productivity proportion of tasarculture in terms of seeds, cocoons and essentially raw silk, need attention for its vital role in reforming the livelihood and economic condition of tribal farmers. The conventional approaches on basic stock maintenance, commercial seed production, selective use of parental races or parents for heterosis and heterobeltiosis, method of backcrossing to exploit the traits of commercial importance and applying the advantage of genotype x environment (G x E) interactions are indispensable. In spite of current knowledge on sophisticated transgenic silkworm, appropriate application of on-hand parental resource material and methodologies can expedite tasar silk productivity improvement in addition to up-keep of the agro based cottage industry’s cost-effectiveness

Genomic introgression mapping of field-derived multiple-anthelmintic resistance in Teladorsagia circumcincta

Preventive chemotherapy has long been practiced against nematode parasites of livestock, leading to widespread drug resistance, and is increasingly being adopted for eradication of human parasitic nematodes even though it is similarly likely to lead to drug resistance. Given that the genetic architecture of resistance is poorly understood for any nematode, we have analyzed multidrug resistant Teladorsagia circumcincta, a major parasite of sheep, as a model for analysis of resistance selection. We introgressed a field-derived multiresistant genotype into a partially inbred susceptible genetic background (through repeated backcrossing and drug selection) and performed genome-wide scans in the backcross progeny and drug-selected F2 populations to identify the major genes responsible for the multidrug resistance. We identified variation linking candidate resistance genes to each drug class. Putative mechanisms included target site polymorphism, changes in likely regulatory regions and copy number variation in efflux transporters. This work elucidates the genetic architecture of multiple anthelmintic resistance in a parasitic nematode for the first time and establishes a framework for future studies of anthelmintic resistance in nematode parasites of humans

Economic Impact Analysis of Marker-Assisted Breeding in Rice

The benefits of developing and releasing salinity-tolerant and phosphorous-deficiency-tolerant rice in Bangladesh, India, Indonesia and the Philippines were estimated for marker-assisted breeding as compared to conventional breeding using economic surplus analysis. Marker-assisted breeding is estimated to save at least 2 to 3 years in the breeding cycle and result in incremental benefits over 25 years in the range of $300 to$800 million depending on the country, stress, and time lags. Salinity and phosphorous deficient soils are difficult problems to solve through conventional breeding because of “genetic load” or undesirable traits that accompany desirable ones during backcrossing. MAB, enabled by advances in genomics and molecular mapping is more precise and hence time-saving. Solving salinity and P-deficiency problems is important, regardless of whether MAB or CB is used, as the cumulative benefits are at least $220 million and as much as$4 billion over the next 25 years depending on the problem and country.Crop Production/Industries,

Acaricide resistance and genetic affinities of some selected populations of Tetranychus urticae Koch in New Zealand : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science (Horticultural) in Entomology at Massey University

A study of resistance to acaricides in a number of populations of the two-spotted spider mite, Tetranychus urticae, in New Zealand had been carried out. Natural genetic and cytoplasmic incompatibilities between populations were also investigated with a view to possible biological control of the pest. Facets of acaricide resistance that were studied included multi-resistance, cross-resistance, negatively correlated resistance and the inheritance of resistance. Chemicals used included an organophosphate representative (parathion-methyl), a carbamate (formetanate), an ungrouped compound (tricyclohexyltin hydroxide) and an organochlorine (dicofol). Cross-resistance was demonstrated between parathion-methyl and formetanate in five populations obtained from widely separate areas of New Zealand. The resistance to parathion of three strains was found to be inherited as a single dominant character and transmissible by both sexes. Cytoplasmic factors (or nucleo-cytoplasmic interactions) and minor genes were found to contribute slightly to the expression of total resistance. No resistance to tricyclohexyltin hydroxide (Plictran) and dicofol (Kelthane) was detected. High degrees of incompatibility (haploid egg lethality) were observed in the hybrids of crosses between the various populations. Chromosomal rearrangements in balanced, heterozygous conditions, in conjunction with the cytoplasm, were considered to be important factors determining the interpopulational sterilities. The interpopulational incompatibility phenomenon was found to be multi-factorial and not associated with the resistance factor. The egg mortalities of some backcross series which remained constantly high in spite of several crossings, implicated that the introduction of normal males to a resistant mite population in an enclosed area (e.g. in a glasshouse) might be a worthwhile proposition in the integrated control of spider mites. Backcross hybrids, on allowing to multiply randomly, were capable of forming new gene combinations, leading consequently to the formation of new strains which were genetically different from the original parents used in the backcross series

Host resistance does not explain variation in incidence of male-killing bacteria in Drosophila bifasciata

Background Selfish genetic elements that distort the sex ratio are found widely. Notwithstanding the number of records of sex ratio distorters, their incidence is poorly understood. Two factors can prevent a sex ratio distorter from invading: inability of the sex ratio distorter to function (failure of mechanism or transmission), and lack of drive if they do function (inappropriate ecology for invasion). There has been no test to date on factors causing variation in the incidence of sex ratio distorting cytoplasmic bacteria. We therefore examined whether absence of the male-killing Wolbachia infection in D. bifasciata in Hokkaido island of Japan, in contrast to the presence of infection on the proximal island of Honshu, was associated with failure of the infection to function properly on the Hokkaido genetic background. Results The male-killer both transmitted and functioned well following introgression to each of 24 independent isofemale inbred lines carrying Hokkaido genetic backgrounds. This was maintained even under stringent conditions of temperature. We therefore reject the hypothesis that absence of infection is due to its inability to kill males and transmit on the Hokkaido genetic background. Further trap data indicates that D. bifasciata may occur at different densities in Hokkaido and Honshu populations, giving some credence to the idea that ecological differentiation could be important. Conclusions The absence of the infection from the Hokkaido population is not caused by failure of the male-killer to function on the Hokkaido genetic background

A major QTL corresponding to the Rk locus for resistance to root-knot nematodes in cowpea (Vigna unguiculata L. Walp.).

Key messageGenome resolution of a major QTL associated with the Rk locus in cowpea for resistance to root-knot nematodes has significance for plant breeding programs and R gene characterization. Cowpea (Vigna unguiculata L. Walp.) is a susceptible host of root-knot nematodes (Meloidogyne spp.) (RKN), major plant-parasitic pests in global agriculture. To date, breeding for host resistance in cowpea has relied on phenotypic selection which requires time-consuming and expensive controlled infection assays. To facilitate marker-based selection, we aimed to identify and map quantitative trait loci (QTL) conferring the resistance trait. One recombinant inbred line (RIL) and two F2:3 populations, each derived from a cross between a susceptible and a resistant parent, were genotyped with genome-wide single nucleotide polymorphism (SNP) markers. The populations were screened in the field for root-galling symptoms and/or under growth-chamber conditions for nematode reproduction levels using M. incognita and M. javanica biotypes. One major QTL was mapped consistently on linkage group VuLG11 of each population. By genotyping additional cowpea lines and near-isogenic lines derived from conventional backcrossing, we confirmed that the detected QTL co-localized with the genome region associated with the Rk locus for RKN resistance that has been used in conventional breeding for many decades. This chromosomal location defined with flanking markers will be a valuable target in marker-assisted breeding and for positional cloning of genes controlling RKN resistance

Comparative susceptibility of mosquito populations in North Queensland, Australia to oral infection with dengue virus.

Dengue is the most prevalent arthropod-borne virus, with at least 40% of the world's population at risk of infection each year. In Australia, dengue is not endemic, but viremic travelers trigger outbreaks involving hundreds of cases. We compared the susceptibility of Aedes aegypti mosquitoes from two geographically isolated populations to two strains of dengue virus serotype 2. We found, interestingly, that mosquitoes from a city with no history of dengue were more susceptible to virus than mosquitoes from an outbreak-prone region, particularly with respect to one dengue strain. These findings suggest recent evolution of population-based differences in vector competence or different historical origins. Future genomic comparisons of these populations could reveal the genetic basis of vector competence and the relative role of selection and stochastic processes in shaping their differences. Lastly, we show the novel finding of a correlation between midgut dengue titer and titer in tissues colonized after dissemination