Cartilage Appearance Using an Environmental Scanning Electron Microscope.

Because of technical principles, samples to be observed with electron microscopy need to be fixed in a chemical process and exposed to vacuum conditions that can produce some changes in the morphology of the specimen. The aim of this work was to obtain high-resolution images of the fresh articular cartilage surface with an environmental scanning electron microscope (ESEM), which is an instrument that permits examination of biological specimens without fixation methods in a 10 Torr chamber pressure, thus minimizing the risk of creating artifacts in the structure. Samples from weight-bearing areas of femoral condyles of New Zealand white rabbits were collected and photographed using an ESEM. Images were analyzed using a categorization based in the Jurvelin classification system modified by Hong and Henderson. Appearance of the observed elevations and depressions as described in the classification were observed, but no fractures or splits of cartilage surface, thought to be artifacts, were detected. The ESEM is a useful tool to obtain images of fresh articular cartilage surface appearance without either employing fixation methods or exposing the specimen to extreme vacuum conditions, reducing the risk of introducing artifacts within the specimen. For all these reasons it could become a useful tool for quality control of the preservation process of osteochondral allografting in a bank of musculoskeletal tissues

Hot spots and waves in Bi2Sr2CaCu2O8 intrinsic Josephson junction stacks -a study by Low Temperature Scanning Laser Microscopy

Recently, it has been shown that large stacks of intrinsic Josephson junctions in Bi2Sr2CaCu2O8 emit synchronous THz radiation, the synchronization presumably triggered by a cavity resonance. To investigate this effect we use Low Temperature Scanning Laser Microscopy to image electric field distributions. Apart from verifying the appearance of cavity modes at low bias we find that, in a high input power regime, standing-wave patterns are created through interactions with a hot spot, possibly pointing to a new mode of generating synchronized radiation in intrinsic Josephson junction stacks.Comment: 6 pages, 5 figures, supplementary information include

Domain-adversarial neural networks to address the appearance variability of histopathology images

Preparing and scanning histopathology slides consists of several steps, each with a multitude of parameters. The parameters can vary between pathology labs and within the same lab over time, resulting in significant variability of the tissue appearance that hampers the generalization of automatic image analysis methods. Typically, this is addressed with ad-hoc approaches such as staining normalization that aim to reduce the appearance variability. In this paper, we propose a systematic solution based on domain-adversarial neural networks. We hypothesize that removing the domain information from the model representation leads to better generalization. We tested our hypothesis for the problem of mitosis detection in breast cancer histopathology images and made a comparative analysis with two other approaches. We show that combining color augmentation with domain-adversarial training is a better alternative than standard approaches to improve the generalization of deep learning methods.Comment: MICCAI 2017 Workshop on Deep Learning in Medical Image Analysi

Broken symmetry of row switching in 2D Josephson junction arrays

We present an experimental and theoretical study of row switching in two-dimensional Josephson junction arrays. We have observed novel dynamic states with peculiar percolative patterns of the voltage drop inside the arrays. These states were directly visualized using laser scanning microscopy and manifested by fine branching in the current-voltage characteristics of the arrays. Numerical simulations show that such percolative patterns have an intrinsic origin and occur independently of positional disorder. We argue that the appearance of these dynamic states is due to the presence of various metastable superconducting states in arrays.Comment: 4 Pages, 6 Figure

Influence of Plating Parameter and Surface Morphology on Mild Steel

The plating parameter effect of zinc deposition on mild steel substrates was investigated. The results showed an improved surface finished and homogeneous layer. The distance between the anode and the cathode on voltage, plating time and coating thickness were considered. The mild steel was deposited into solution of zinc bath for varying voltage between 0.5 v and 1.0v. It was discovered that the sample plated at 0.8v for 15 minutes gives the best plating deposition and appearance. The surface morphology of the plated sample was analyzed by Focused ion beam scanning electron microscopy (FIB-SEM), AFM and X-ray diffraction