Three-dimensional double helical DNA structure directly revealed from its X-ray fiber diffraction pattern by iterative phase retrieval

Coherent diffraction imaging (CDI) allows the retrieval of the structure of an isolated object, such as a macromolecule, from its diffraction pattern. CDI requires the fulfilment of two conditions: the imaging radiation must be coherent and the object must be isolated. We discuss that it is possible to directly retrieve the molecular structure from its diffraction pattern which was acquired neither with coherent radiation nor from an individual molecule, provided the molecule exhibits periodicity in one direction, as in the case of fiber diffraction. We demonstrate that by applying iterative phase retrieval methods to a fiber diffraction pattern, the repeating unit, that is, the molecule structure, can directly be reconstructed without any prior modeling. As an example, we recover the structure of the DNA double helix in three-dimensions from its two-dimensional X-ray fiber diffraction pattern, Photograph 51, acquired in the famous experiment by Raymond Gosling and Rosalind Franklin, at a resolution of 3.4 Angstrom

Towards optical intensity interferometry for high angular resolution stellar astrophysics

Most neighboring stars are still detected as point sources and are beyond the angular resolution reach of current observatories. Methods to improve our understanding of stars at high angular resolution are investigated. Air Cherenkov telescopes (ACTs), primarily used for Gamma-ray astronomy, enable us to increase our understanding of the circumstellar environment of a particular system. When used as optical intensity interferometers, future ACT arrays will allow us to detect stars as extended objects and image their surfaces at high angular resolution. Optical stellar intensity interferometry (SII) with ACT arrays, composed of nearly 100 telescopes, will provide means to measure fundamental stellar parameters and also open the possibility of model-independent imaging. A data analysis algorithm is developed and permits the reconstruction of high angular resolution images from simulated SII data. The capabilities and limitations of future ACT arrays used for high angular resolution imaging are investigated via Monte-Carlo simulations. Simple stellar objects as well as stellar surfaces with localized hot or cool regions can be accurately imaged. Finally, experimental efforts to measure intensity correlations are expounded. The functionality of analog and digital correlators is demonstrated. Intensity correlations have been measured for a simulated star emitting pseudo-thermal light, resulting in angular diameter measurements. The StarBase observatory, consisting of a pair of 3 m telescopes separated by 23 m, is described.Comment: PhD dissertatio

Reflectance Hashing for Material Recognition

We introduce a novel method for using reflectance to identify materials. Reflectance offers a unique signature of the material but is challenging to measure and use for recognizing materials due to its high-dimensionality. In this work, one-shot reflectance is captured using a unique optical camera measuring {\it reflectance disks} where the pixel coordinates correspond to surface viewing angles. The reflectance has class-specific stucture and angular gradients computed in this reflectance space reveal the material class. These reflectance disks encode discriminative information for efficient and accurate material recognition. We introduce a framework called reflectance hashing that models the reflectance disks with dictionary learning and binary hashing. We demonstrate the effectiveness of reflectance hashing for material recognition with a number of real-world materials