IEEE Trans Instrum Meas

This paper introduces a novel compact low-power amperometric instrumentation design with current-to-digital output for electrochemical sensors. By incorporating the double layer capacitance of an electrochemical sensor's impedance model, our new design can maintain performance while dramatically reducing circuit complexity and size. Electrochemical experiments with potassium ferricyanide, show that the circuit output is in good agreement with results obtained using commercial amperometric instrumentation. A high level of linearity (R| = 0.991) between the circuit output and the concentration of potassium ferricyanide was also demonstrated. Furthermore, we show that a CMOS implementation of the presented architecture could save 25.3% of area, and 47.6% of power compared to a traditional amperometric instrumentation structure. Thus, this new circuit structure is ideally suited for portable/wireless electrochemical sensing applications.20192021-05-01T00:00:00ZR01 ES022302/ES/NIEHS NIH HHS/United StatesR01 OH009644/OH/NIOSH CDC HHS/United States32292210PMC7156046759

An amperometric glucose biosensor with enhanced measurement stability and sensitivity using an artificially porous conducting polymer

A conducting polymer [polypyrrole (PPy)]-based amperometric biosensor fabricated on a platinum-coated nanoporous alumina electrode has been described. This fabricating process introduced artificial porosity into the PPy film, and the template pore sizes were carefully chosen to match the size of the glucose oxidase (GOx) molecule. The PF−6 -doped PPy film was synthesized with 0.05 M pyrrole and 0.1 M NaPF6 at a current density of 0.3 mA/cm2 for 90 s. Immobilization was done by physically adsorbing 5 μL of GOx on the nanoporous PPy film. Glutaraldehyde (0.1 wt.%, 5 μL) was used for cross-linking. The synthesized films were characterized by using an electrochemical technique and scanning electron microscopy (SEM). Amperometric responses were measured as a function of different concentrations of glucose at 0.4 V. Nanoporous electrodes lead to high enzyme loading, whereas the use of a cross-linking agent increased stability, sensitivity, reproducibility, repeatability, and shelf life

An amperometric glucose biosensor with enhanced measurement stability and sensitivity using an artificially porous conducting polymer

A conducting polymer [polypyrrole (PPy)]-based amperometric biosensor fabricated on a platinum-coated nanoporous alumina electrode has been described. This fabricating process introduced artificial porosity into the PPy film, and the template pore sizes were carefully chosen to match the size of the glucose oxidase (GOx) molecule. The PF−6 -doped PPy film was synthesized with 0.05 M pyrrole and 0.1 M NaPF6 at a current density of 0.3 mA/cm2 for 90 s. Immobilization was done by physically adsorbing 5 μL of GOx on the nanoporous PPy film. Glutaraldehyde (0.1 wt.%, 5 μL) was used for cross-linking. The synthesized films were characterized by using an electrochemical technique and scanning electron microscopy (SEM). Amperometric responses were measured as a function of different concentrations of glucose at 0.4 V. Nanoporous electrodes lead to high enzyme loading, whereas the use of a cross-linking agent increased stability, sensitivity, reproducibility, repeatability, and shelf life