9,612 research outputs found
Recent development of prebiotic research — statement from an expert workshop
A dietary prebiotic is defined as ‘a substrate that is selectively utilized by host microorganisms conferring a health benefit’. Although this definition evolved concomitantly with the knowledge and technological developments that accrued in the last twenty years, what qualifies as prebiotic continues to be a matter of debate. In this statement, we report the outcome of a workshop where academic experts working in the field of prebiotic research met with scientists from industry. The workshop covered three main topics: (i) evolution of the prebiotic concept/definition; (ii) the gut modeling in vitro technology PolyFermS to study prebiotic effects; and (iii) the potential novel microbiome-modulating effects associated with vitamins. The future of prebiotic research is very promising. Indeed, the technological developments observed in recent years provide scientists with powerful tools to investigate the complex ecosystem of gut microbiota. Combining multiple in vitro approaches with in vivo studies is key to understanding the mechanisms of action of prebiotics consumption and their potential beneficial effects on the host
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Selective incorporation of proteinaceous over nonproteinaceous cationic amino acids in model prebiotic oligomerization reactions.
Numerous long-standing questions in origins-of-life research center on the history of biopolymers. For example, how and why did nature select the polypeptide backbone and proteinaceous side chains? Depsipeptides, containing both ester and amide linkages, have been proposed as ancestors of polypeptides. In this paper, we investigate cationic depsipeptides that form under mild dry-down reactions. We compare the oligomerization of various cationic amino acids, including the cationic proteinaceous amino acids (lysine, Lys; arginine, Arg; and histidine, His), along with nonproteinaceous analogs of Lys harboring fewer methylene groups in their side chains. These analogs, which have been discussed as potential prebiotic alternatives to Lys, are ornithine, 2,4-diaminobutyric acid, and 2,3-diaminopropionic acid (Orn, Dab, and Dpr). We observe that the proteinaceous amino acids condense more extensively than these nonproteinaceous amino acids. Orn and Dab readily cyclize into lactams, while Dab and Dpr condense less efficiently. Furthermore, the proteinaceous amino acids exhibit more selective oligomerization through their α-amines relative to their side-chain groups. This selectivity results in predominantly linear depsipeptides in which the amino acids are α-amine-linked, analogous to today's proteins. These results suggest a chemical basis for the selection of Lys, Arg, and His over other cationic amino acids for incorporation into proto-proteins on the early Earth. Given that electrostatics are key elements of protein-RNA and protein-DNA interactions in extant life, we hypothesize that cationic side chains incorporated into proto-peptides, as reported in this study, served in a variety of functions with ancestral nucleic acid polymers in the early stages of life
Toward homochiral protocells in noncatalytic peptide systems
The activation-polymerization-epimerization-depolymerization (APED) model of
Plasson et al. has recently been proposed as a mechanism for the evolution of
homochirality on prebiotic Earth. The dynamics of the APED model in
two-dimensional spatially-extended systems is investigated for various
realistic reaction parameters. It is found that the APED system allows for the
formation of isolated homochiral proto-domains surrounded by a racemate. A
diffusive slowdown of the APED network such as induced through tidal motion or
evaporating pools and lagoons leads to the stabilization of homochiral bounded
structures as expected in the first self-assembled protocells.Comment: 10 pages, 5 figure
Nitrogen Oxide Concentrations in Natural Waters on Early Earth
A key challenge in origins-of-life studies is estimating the abundances of
species relevant to the chemical pathways proposed to have contributed to the
emergence of life on early Earth. Dissolved nitrogen oxide anions
(NO), in particular nitrate (NO) and nitrite
(NO), have been invoked in diverse origins-of-life chemistry, from
the oligomerization of RNA to the emergence of protometabolism. Recent work has
calculated the supply of NO from the prebiotic atmosphere to the
ocean, and reported steady-state [NO] to be high across all plausible
parameter space. These findings rest on the assumption that NO is
stable in natural waters unless processed at a hydrothermal vent. Here, we show
that NO is unstable in the reducing environment of early Earth. Sinks
due to UV photolysis and reactions with reduced iron (Fe) suppress
[NO] by several orders of magnitude relative to past predictions. For
pH and C, we find that it is most probable that
NO]M in the prebiotic ocean. On the other hand, prebiotic
ponds with favorable drainage characteristics may have sustained
[NO]M. As on modern Earth, most NO on prebiotic
Earth should have been present as NO, due to its much greater
stability. These findings inform the kind of prebiotic chemistries that would
have been possible on early Earth. We discuss the implications for proposed
prebiotic chemistries, and highlight the need for further studies of
NO kinetics to reduce the considerable uncertainties in predicting
[NO] on early Earth.Comment: In review for publication at Geochemistry, Geophysics, and Geosystems
(G-cubed). Comments, questions, and criticism solicited; please contact
corresponding author at [email protected]. SI at:
https://web-cert.mit.edu/sukrit/Public/nox_si.pdf. GitHub at:
https://github.com/sukritranjan/no
Modern views of ancient metabolic networks
Metabolism is a molecular, cellular, ecological and planetary phenomenon, whose fundamental principles are likely at the heart of what makes living matter different from inanimate one. Systems biology approaches developed for the quantitative analysis of metabolism at multiple scales can help understand metabolism's ancient history. In this review, we highlight work that uses network-level approaches to shed light on key innovations in ancient life, including the emergence of proto-metabolic networks, collective autocatalysis and bioenergetics coupling. Recent experiments and computational analyses have revealed new aspects of this ancient history, paving the way for the use of large datasets to further improve our understanding of life's principles and abiogenesis.https://www.sciencedirect.com/science/article/pii/S2452310017302196Published versio
Identification of the prebiotic translation apparatus within the contemporary ribosome
A structural element that could have existed independently in the prebiotic era was identified at the active site of the contemporary ribosome. It is suggested to have functioned as a proto-ribosome catalyzing peptide bond formation and non-coded elongation in the same manner that contemporary ribosomes exert positional catalysis, namely by accommodating the reactants in stereochemistry favourable for inline nucleophilic attack. This simple apparatus is a dimer of self-folding RNA units that could have assembled spontaneously into a symmetrical pocket-like structure, sufficiently efficient to be preserved throughout evolution as the active site of modern ribosomes, thus presenting a conceivable starting point for translation.Here we discuss the proto-ribosome emergence hypothesis and show that the tendency for dimerization, a prerequisite for obtaining the catalytic centre, is linked to the fold of its two components, indicating functional selection at the molecular level in the prebiotic era and supporting the existence of dimeric proto-ribosome
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