A novel prediction algorithm of thermostable proteins by using hurst exponent and choquet integral regression model

[[abstract]]In search of good prediction algorithm of thermostable proteins is an important issue. In this paper, a novel prediction algorithm of thermostable proteins by using Hurst exponent and Choquet integral regression model based on ?measure and ?support is proposed. This method not used before is the first one integrating the physicochemical properties, fractal property and Choquet integral regression model. For evaluating the performance of this new algorithm, a real data experiment by using Leaveoneout CrossValidation accuracy is conducted. Experimental result shows that this new classification algorithm is useful and batter than those methods by using Hurst exponent and the traditional prediction models, multiple regression model and ridge regression model. © 2008 IEEE

Thermostability in endoglucanases is fold-specific

<p>Abstract</p> <p>Background</p> <p>Endoglucanases are usually considered to be synergistically involved in the initial stages of cellulose breakdown-an essential step in the bioprocessing of lignocellulosic plant materials into bioethanol. Despite their economic importance, we currently lack a basic understanding of how some endoglucanases can sustain their ability to function at elevated temperatures required for bioprocessing, while others cannot. In this study, we present a detailed comparative analysis of both thermophilic and mesophilic endoglucanases in order to gain insights into origins of thermostability. We analyzed the sequences and structures for sets of endoglucanase proteins drawn from the Carbohydrate-Active enZymes (CAZy) database.</p> <p>Results</p> <p>Our results demonstrate that thermophilic endoglucanases and their mesophilic counterparts differ significantly in their amino acid compositions. Strikingly, these compositional differences are specific to protein folds and enzyme families, and lead to differences in intramolecular interactions in a fold-dependent fashion.</p> <p>Conclusions</p> <p>Here, we provide fold-specific guidelines to control thermostability in endoglucanases that will aid in making production of biofuels from plant biomass more efficient.</p