Collagenous Matrix Supported by A 3D-Printed Scaffold for Osteogenic Differentiation of Dental Pulp Cells

Objective A systematic characterization of hybrid scaffolds, fabricated based on combinatorial additive manufacturing technique and freeze-drying method, is presented as a new platform for osteoblastic differentiation of dental pulp cells (DPCs). Methods The scaffolds were consisted of a collagenous matrix embedded in a 3D-printed beta-tricalcium phosphate (β-TCP) as the mineral phase. The developed construct design was intended to achieve mechanical robustness owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the Collagen extracellular matrix. The β-TCP precursor formulations were investigated for their flow-ability at various temperatures, which optimized for fabrication of 3D printed scaffolds with interconnected porosity. The hybrid constructs were characterized by 3D laser scanning microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and compressive strength testing. Results The in vitro characterization of scaffolds revealed that the hybrid β-TCP/Collagen constructs offer superior DPCs proliferation and alkaline phosphatase (ALP) activity compared to the 3D-printed β-TCP scaffold over three weeks. Moreover, it was found that the incorporation of TCP into the Collagen matrix improves the ALP activity. Significance The presented results converge to suggest the developed 3D-printed β-TCP/Collagen hybrid constructs as a new platform for osteoblastic differentiation of DPCs for craniomaxillofacial bone regeneration

3D Printed Sports Mouthguard

The 3D printed sports mouthguard can be specifically designed to provide exceptional protection and comfort to athletes in any sport. The process by which an athlete undergoes for obtaining their mouthguard requires a substantial amount of time and cost. Introducing 3D printer capabilities into the dental and sporting fields would elicit faster manufacturing time with more economically priced materials. In testing our theory, the initial components of the research consisted of scanning a dental impression cast and transferring the S.T.L. file scan onto the C.A.D. software. The next important step required us to discover which material to wire with the 3D printer so that the first figure could be presented. After the first test material was chosen, we had to manipulate the measurements so that the 3D printer could properly layer the material to form a practical design. We then had to search for materials that adhered to the A.D.A. and the F.D.A.’s requirements for safety as this product is a protective oral device. Although we were unable to provide a fully functional 3D printed sports mouthguard example due to time constraints, we were able to provide a strong foundation for companies which would be willing to invest and apply this research.https://scholarscompass.vcu.edu/capstone/1205/thumbnail.jp

Evaluation of fit for 3D printed retainers as compared to thermoform retainers

ABSTRACT EVALUATION OF FIT FOR 3D PRINTED RETAINERS AS COMPARED TO THERMOFORM RETAINERS By David Cole, D.M.D. A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Dentistry at Virginia Commonwealth University Thesis Directors: Eser Tüfekçi, D.D.S., M.S., Ph.D., M.S.H.A. Professor, Department of Orthodontics Sompop Bencharit, D.D.S., M.S., Ph.D. Associate Professor and Director of Digital Dentistry, Department of General Practice Introduction: Despite recent advances in three-dimensional (3D) printing, little information is available on 3D printed retainers Methods: Three reference models were used to fabricate traditional vacuum formed, commercially-available vacuum formed, and 3D printed retainers. For each model, three retainers were made using the three methods (a total of 27 retainers). To determine the trueness, the distances between the intaglio surface of the retainers and the occlusal surface of the reference models were measured using an engineering software. A small difference was indicative of a good fit. Results: Average differences of the traditional vacuum formed retainers ranged from 0.10 to 0.20mm. The commercially-available and 3D printed retainers had a range of 0.10 to 0.30mm and 0.10 to 0.40mm, respectively. Conclusions: The traditional vacuum formed retainers showed the least amount of deviation from the original reference models while the 3D printed retainers showed the greatest deviation

Fatigue strength of additively manufactured polylactide (PLA): effect of raster angle and non-zero mean stresses

As far as polymers are concerned, polylactide (PLA) is certainly the polymeric compound that is most commonly used along with commercial additive manufacturing technologies. In this context, the present paper aims to investigate the influence of manufacturing direction and superimposed static stresses on the fatigue strength of PLA 3D-printed by using the Fused Filament Fabrication technique. This was done not only by generating a large number of new experimental results, but also by re-analysing different data sets taken from the technical literature. As long as the fused deposition modelling technology is used to fabricate, flat on the build-plate, objects of PLA, the obtained results and the performed re-analyses allowed us to come to the following conclusions: (i) the influence of the manufacturing direction can be neglected with little loss of accuracy; (ii) the effect of superimposed static stresses can be quantified and assessed effectively by simply performing the fatigue assessment in terms of maximum stress in the cycle; (iii) if appropriate experiments cannot be run, AM PLA can be designed against fatigue (for a probability of survival larger than 90%) by referring to a unifying design curve having negative inverse slope equal to 5.5 and endurance limit (at =2∙106 cycles to failure) equal to 10% of the material ultimate tensile strength

Exploring the Abilities of 3D Printing and its Viability for Consumption in the Fashion Industry

Abstract With the ever-evolving state of today’s technology, designers and retailers in the apparel industry are seeking out new technological methods that have the capacity to revolutionize and individualize their brand, as well as meet consumer needs and preferences. An emerging technology is 3D printing, which utilizes computer-aided technology and a variety of filaments to construct an object. Though 3D printing technology offers the ability for rapid prototyping, a condensed supply chain, and a sustainable additive manufacturing process, there is question as to whether or not consumers are ready for 3D printed clothing to enter their wardrobes. In this creative study, the authors designed a 3D printed garment in order to test whether 3D printers could be used to make wearable clothing of similar characteristics to clothing typically made of fabric. A survey was then conducted on the University of Arkansas campus to measure consumer response to the project garment. Three primary factors were measured: prior exposure and interest in 3D printing, general fashion interest, and aesthetic appeal of the project 3D printed garment. Overall perceptions of the project garment as well as further use of 3D printing for the apparel industry were positive. The ability of this study to create a fully 3D printed garment as well as understand consumer response to 3D printed clothing provides insight into this emerging technology. The results warrant further research into its capabilities for fashion and that the fashion industry could move towards adopting this technology on a wider scale in coming years. The results indicate that a major transformation in ready-to-wear style is feasible and beneficial to the apparel industry because of 3D printing. Keywords: 3D printing, fashion, consumer preference, sustainability, apparel, technolog

Characterisation of Cryogenic Material Properties of 3D-Printed Superconducting Niobium using a 3D Lumped Element Microwave Cavity

We present an experimental characterisation of the electrical properties of 3D-printed Niobium. The study was performed by inserting a 3D-printed Nb post inside an Aluminium cylindrical cavity, forming a 3D lumped element re-entrant microwave cavity resonator. The resonator was cooled to temperatures below the critical temperature of Niobium (9.25K) and then Aluminium (1.2K), while measuring the quality factors of the electromagnetic resonances. This was then compared with finite element analysis of the cavity and a measurement of the same cavity with an Aluminium post of similar dimensions and frequency, to extract the surface resistance of the Niobium post. The 3D-printed Niobium exhibited a transition to the superconducting state at a similar temperature to the regular Niobium, as well as a surface resistance of $3.1\times10^{-4}$ $\Omega$. This value was comparable to many samples of traditionally machined Niobium previously studied without specialised surface treatment. Furthermore, this study demonstrates a simple new method for characterizing the material properties of a relatively small and geometrically simple sample of superconductor, which could be easily applied to other materials, particularly 3D-printed materials. Further research and development in additive manufacturing may see the application of 3D-printed Niobium in not only superconducting cavity designs, but in the innovative technology of the future.Comment: 5 pages, 4 figure