血管内皮细胞生长抑制因子的研究进展

血管内皮细胞生长抑制因子(vascularendothelialgrowthinhibitor,VEGI)是一种新型的血管内皮细胞生长抑制因子,属于TNF超家族,是Ⅱ型跨膜蛋白。重组VEGI不仅可以抑制内皮细胞增殖和诱导血管内皮细胞凋亡,而且可阻止新生血管生成,从而产生抗肿瘤生长的作用。VEGI作为一个内皮细胞产生的血管生成负调控因子可激活JNK、P38MAPA及胱冬肽,也可激活NF-κB,从而诱导内皮细胞的凋亡。VEGI的N段部分缺失可影响其生物活性,具有重要的病理生理意义,在肿瘤生物治疗方面有很大的应用前景

Functions and associated mechanisms of Human tumor necrosis factor-alpha-induced protein 8 family during gastric cancer carcinogenesis

胃癌是消化系统最常见的恶性肿瘤，在世界范围内，胃癌的发病率居恶性肿瘤的第四位，死亡率居恶性肿瘤的第二位。胃癌发生、发展涉及多因素、多步骤、多基因参与的复杂过程，迄今为止，胃癌的病因和发病机制尚未完全阐明。因此，对胃癌发病机制、在不同分子途径在肿瘤发生发展中的作用机制的研究，寻找特异性的生物标志分子，对于早期发现胃癌和探寻新的治疗方法，进而提高胃癌患者生存率和提升生活质量具有重要意义。 TNFAIP8家族是由肿瘤坏死因子α诱导产生的一个蛋白质家族，参与多种生物学过程，并在许多疾病特别是自身免疫性疾病和肿瘤的发生、发展中发挥重要的作用，TNFAIP8、TIPE2、TIPE3是其中的重要成员。TN...Gastric cancer(GC) is a leading cause of global cancer mortality responsible for 700000 deaths annually, and still highly prevalent in many parts of Asia, Eastern Europe and South America. Most patients with GC are diagnosed at advanced disease stages, and overall 5-year survival rates for patients with resectable GC remain low at 10-30% despite clinical advances in surgery and therapy.The elucida...学位：理学博士院系专业：医学院_生理学学号：2452013015431

两种雌激素受体亚型在雌性生殖系统中的不同调控作用

雌激素主要通过雌激素受体α和雌激素受体β在哺乳动物雌性生殖过程中起重要作用。这2种雌激素受体序列不同,在作用方面彼此拮抗。它们对于胚胎着床、蜕膜化和卵泡发育至关重要。这2种受体敲除的小鼠有不同程度的卵巢功能缺陷。雌激素受体与子宫内膜异位症、子宫内膜癌和卵巢癌等妇科疾病的发生也密切相关

The mechanism study of TIPE2 in nicotine mediated anti-inflammatory pathway

“胆碱能抗炎通路”是新近发现的神经-免疫调节通路，其激活可有效减少多种促炎因子的释放，对全身和局部炎症均具有明显抑制作用。尼古丁，作为一种胆碱能受体激动剂，其药理作用与乙酰胆碱ACh相似，可有效减轻炎症反应。已有文献报道尼古丁能够降低促炎因子所引起的细胞因子TNF-α、IL-12、IL-6的释放。但到目前为止，尼古丁抗炎通路的分子机制尚不清楚。TIPE2是最近发现的一种免疫负调节分子，属于TNFAIP8家族，主要表达在胸腺、脾脏、淋巴结以及小肠粘膜等免疫器官和淋巴组织中，对适应性免疫和固有免疫起到负性调控作用。但TIPE2是否参与尼古丁所介导的抗炎过程，以及在其中可能所起的作用及机制尚没有文献...“Cholinergic anti-inflammatory pathway” is a newly discovered nerve-immune regulation pathway. Its activation can effectively reduce the release of a variety of pro-inflammatory cytokines and significantly inhibit systemic and local inflammation. Nicotine is an acetylcholine receptor agonist, and its pharmacological effect is similar to acetylcholine Ach, which exerts its effect by acting on choli...学位：理学硕士院系专业：医学院基础医学系_微生物学学号：2452009115300

The Effects of Kv1.3 Potassium Channel on Atherosclerosis Induced by Group IIA Secretory Phospholipase A2

研究背景和目的：IIA型分泌型磷脂酶A2（sPLA2-IIA）作为一类磷脂酶可通过其酶的催化作用和本身的致炎症作用而促进动脉粥样硬化（AS）的发生发展。Kv1.3钾通道活性增高与AS相关性疾病密切相关。二者均可通过调节树突状细胞（DCs）的分化与抗原递呈功能参与AS的发生发展，然而二者之间的相互关系如何尚待进一步阐明。本研究分别在细胞水平和组织水平上探讨Kv1.3钾通道在诱导动脉粥样硬化形成过程中的作用。 方法：1.在单核细胞分化为树突状细胞（DCs）的过程中，用不同浓度sPLA2-IIA干预，观察Kv1.3钾通道表达的变化；阻断Kv1.3钾通道对共刺激分子表达的影响。2.在树突状细胞分化为...Background & Objective: Group IIA Secretory Phospholipase A2 (sPLA2-IIA) aggravated atherosclerosis (AS) progress via itself catalysis and pro-inflammation. Upregulating Kv1.3 potassium channel activity was closely bound up with AS. Both sPLA2-IIA and Kv1.3 potassium channel regulated the differentiation and antigen-presenting function of dendritic cells (DCs), thus participating in the developmen...学位：博士后院系专业：生命科学学院_生物学学号：201117002

植物抗逆性的获得与信息传导

概述了逆境下植物细胞水平的信号传导和生理反应及其相关基因表达的研究进展 ,并着重讨论了植物抗逆性获得与基因组DNA水平分子信号传递的可能机理。提出植物抗逆性获得的细胞分子生物学机制的模型。国家自然科学基金;福建省自然科学基金重点项目资助课题

Quantitative analysis of the developmental potential of cells and tissues based on evolutionary conservation of genes and regulatory regions

Objective·To study the relationship between evolution and the developmental process from the perspective of DNA sequence conservation, and explore their inherent principles.Methods·First, conservation rate (CR) was established by analyzing the conservation of amino acid sequences of coding genes in 100 species to quantify the evolutionary conservation of genes. The relationship between CR and developmental potential was verified by using the feature genes involved in embryonic stem cells pathways. Secondly, cell type-specific genes and their characteristics in conservation were studied by analyzing the RNA sequencing (RNA-seq) data of the three early germ layers (ectoderm, mesoderm and endoderm) and their corresponding mature organs (brain, heart, liver, etc). Then, chromatin immunoprecipitation sequencing (ChIP-seq) data of enhancer histone H3 acetylated at lysine 27 (H3K27ac) from early germ layers and mature organs were collected to search for enhancer sites and identify super enhancers in various cells and tissues by using the ROSE procedure. Functional enrichment and signaling pathway analysis of genes was used to examine the identity correlation between SEs-regulated genes and the corresponding cell characteristics, to clarify whether the SEs identified in this study were consistent with the characteristics reported in previous studies. Finally, PhastCons program was used to calculate the DNA conservation score (CS) of non-coding regulatory regions to study their relationship with developmental potential.Results·In the coding region of DNA, CR was successfully established to quantify the conservation of genes. The gene expression data of early germ layers and mature organs showed that the genes with higher conservation rate were more relevant to the stemness and early developmental process, and the differences between the tissues from early and late development could be distinguished by using CR. In the non-coding regions of DNA, it was found that the conservation of regulatory regions was also correlated with development. The CS of the SE sequences in the early developmental germ layers was significantly higher than that of the SE sequences in the corresponding mature organs. However, cell-specific typical enhancers (TEs) did not show such a trend.Conclusion·During the developmental process, CR of genes expressed in the coding region decreases, and CS of super-enhancer DNA in the non-coding region decreases