Microalgae are amongst the most promising renewable feedstocks for biodiesel production. Control and optimization of the microalgae growth stage can improve the competitiveness and sustainability of microalgal-derived biodiesel industry. The main objective of this work is the development of a predictive microalgae growth model, which considers the impact of growth-associated parameters such as substrate, nitrogen, light and pH. A multi-parameter predictive microalgae growth model has been developed to describe the biomass growth and the lipid accumulation in bench-scale batch systems. Consequently, experiments have been conducted at a range of conditions to estimate the kinetic parameters of the model. The model was fitted to data from lab-scale batch experiments, using 2.1 gL−1 acetic acid and 0.378 gL−1 nitrogen under constant light illumination of 125 μEm−2s−1. The predictiveness of the model was tested by computing outputs of experiments at different conditions: 1.05 gL−1 acetic acid and 0.378 gL−1 nitrogen, under the same light illumination. The validated model can then be exploited to compute optimal operating conditions of bench-scale batch experiments
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