Data about the effect of short-chain PFAS exposure during gestation and breastfeeding on alteration of learning and memory in adulthood

Abstract

The dataset includes detailed outcomes of in vivo and ex vivo tests, aimed to investigate the effect of short-chain Per- and polyfluoroalkyl substances (PFAS) maternal exposure on neurodevelopment in rats. Animals have been exposed to two different contaminants (PFBA, Perfluorobutanoic acid; and GenX, Ammonium Perfluoro, 2-methyl-3-oxahexanoate) at two different doses (indicated as LD, low dose, and HD, high dose) indirectly, through the maternal exposure (4 weeks pre-gestation, gestation, and lactation periods). Tests include a battery of standardized behavioral performed during adult life (9 weeks after weaning), assays designed to probe distinct facets of neurological function. Motor coordination and balance were quantitatively assessed via the Rotarod test. Spontaneous locomotion, reflecting exploratory behavior and anxiety-like states, was evaluated through open-field activity. Cognitive and mnemonic abilities were thoroughly investigated using the Morris water maze. In vivo analysis have been implemented with quantification of circulating hormones and gene expression data of neurogenesis marker in the hippocampus. Furthermore, the dataset integrates cell culture data from ex vivo studies, using primary hippocampl neurons (isolated from P0 pups exposed to PFBA during gestation) providing a comprehensive view. This dataset also includes quantitative analyses of short-chain PFAS residues in key tissues (liver, kidneys, brain, and thyroid)