Data about the role of RXRγ in T3-mediated oligodendrocyte precursor cell differentiation

Abstract

This dataset contains data describing the effect of the knock-out of the Rxrg gene in neral stem cell (NSC)-derived oligodendrocyte precursor cells (OPCs) differentiation and maturation. Data has been obtained by transcriptomic analysis using RNAseq in cells isolated by WT and Rxrg-/- animals cultured as indifferentiated precursors and 24 hours after the differentiation induction by triiodotironine (T3) exposure, mimiking the physiologic differentiation/maturation molecular machinery. RNAseq data has been validated using morphological analysis. Molecular tools has been used to inhibit (cyclopamine, GANT61) or activate (SAG, purmorphamine) the sonic hadgehog (SHH) pathway in differentiating WT and Rxrg-/- OPC cultures. Specific markers (NG2, MBP) have been used to quantify the differentiation/maturation of the OPC culture, measuring the percentage of precursors and mature oligodendrocytes