This dataset contains RNAseq data to measure gene expression and identify differentially expressed genes (DEGs) in Mediterranean mussels (Mytilus galloprovincialis) exposed for 3 and 30 days to experimental heat waves (28°C), mimiking the temperature increase expected in 2050. The control group was maintained at a temperature of 20.0 ± 0.5 ◦C, while the exposed group was brought to 28.0 ± 0.5 °C by warming the water at a rate of 0.5 °C·h-1. The water temperatures were kept constant for 30 days using electronic thermostats and heat exchangers. Seawater parameters (temperature, salinity, and pH) were checked daily
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