Genome Analysis of Aeromonas hydrophila strain S14-452

Abstract

The Aeromonas hydrophila are gram-negative, opportunistic bacteria that can infect many groups of organisms, mainly fish and amphibians. In channel catfish, A. hydrophila causes fatal bacterial septicemia, leading to massive economic losses for catfish farmers. Therefore, an understanding of its genome will aid in identifying its virulence factors, metabolic pathways and survival mechanisms within the host. This study details the draft genome sequence of A. hydrophila strain S14-452, isolated from channel catfish exhibiting bacterial septicemia during a disease outbreak at a catfish farm. A. hydrophila strain S14-452 was sequenced using the Illumina iSeq 100 System. Assembly was performed with SPAdes, producing 218 contigs which were annotated using the Rapid Annotations Subsystem Technology (RAST) server and the SEED viewer. The draft genome comprises 5,047,785 bp and has a G+C content of 60.8%. It has 5,013 predicted genes, of which 4,903 are coding sequences (CDS), 110 tRNA, and 5 rRNA