Isolations and Characterization of Novel Bacterial Species from Cyprinus Carpio and Its Use in Biodegradation of Polystyrene with Emphasis on SDG 3 for Ensuring Healthy Lives and Well-Being for All Humans and Animals

Abstract

Objective: This research aims to investigate the feasibility of employing beneficial bacterial species present in the gastrointestinal tract (GIT) of common carp (Cyprinus carpio) in order to biodegrade microplastics (MP), which can provide healthy lives for both humans and animals.   Theoretical framework: the main concept in this research is that the bacterial communities found in (GIT) of both people and animals can produce beneficial effects. Microorganisms that are beneficial to the host and are typically found in GIT are generally referred to as probiotics: Method: The 16S rRNA sequencing method was utilized to determine the species present in the bacterial community of Cyprinus carpio. The novel species Hafnia parallel UUNT_MP29 was utilized to conduct the biodegradation investigation. PS was used as the sole source of carbon. Discussion and the Results: During the PS batch experiment, this strain reached its highest point on the fifteenth day, displaying an incubation rate of 1.3x108 CFU/mL. Following that, the microbe entered a stationary phase, which it remained in until the experiment was finished on the 20th. According to the results, this strain appears to be a new indigenous variation capable of biodegrading PS compounds. Originality/Value: This study contributes to the existing body of knowledge by discovering bacterial species from common carp that have the potential to degrade PS. The possible application of Hafnia paralvei UUNT_MP29 in the rehabilitation of wastewater polluted by microplastics demonstrates the significance and utility of this research