Investigating the Role of Acacia Nilotica Nanoparticles on Promoting Apoptosis in Human Breast Cancer Cell Line (MDA-MB-231)

Abstract

MDA-MB-231 is a model of a human breast cancer cell line. It represents a suitable cell line for breast cancer research worldwide, including anti-cancer studies. Natural products are rich in phytochemicals that have anti-cancer, antioxidant and anti-inflammatory effects. The aim of this study was to characterize the Acacia nilotica nanoparticles (AN-NPs) from the extract of Acacia nilotica (AN) using transmission electron microscopy (TEM), zeta sizer, X-ray diffraction (XRD) and Fourier transform infrared (FT-IR). Cytotoxic activity was assessed using the 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The morphological changes of the cells were examined using an inverted microscope. The results showed that at serial concentrations (5, 10, 20, 50 and 70 µg/ml) of AN extract and AN-NPs, a cytotoxic effect and morphological degeneration and damage of the cells were observed. The effect varied depending on the exposure time and AN extract and/or AN-NP concentration on MDA-MB-231. The results showed cytotoxic effects, morphological degeneration, damage and more efficacy against breast cancer cells. We can conclude that AN extract and AN-NP are an effective choice for the development of pharmacological treatments against cancer