THE USE OF PHYSIOLOGICALLY ACTIVE SUBSTANCES IN THE BIOSORPTION OF IRON (III) ION

Abstract

This article proposes a simple and rapid method for the spectrophotometric determination of iron (III) using a physiologically active compound - the mono ammonium salt of glycyrrhizic acid (MASGA). The spectral characteristics of the MASGK-Fe (III) complex - absorption maxima (λmax = 440nm) and the actual molar extinction coefficient (ε = 3,425 × 102) were determined. The experimental results showed that when using a universal buffer solution, the complex compound had the maximum optical density. Therefore, in subsequent studies, a universal buffer solution with pH = 5 was used. The experimental results showed that the optical density of the complex compound practically did not change within 2 hours, and after 2 hours a slight decrease was observed. The optimal conditions for the determination of iron (III) in the range of its concentrations of 2,0–45,0 μg / 25 ml of solution have been established. The developed technique was applied to determine iron in model solutions of algae. To establish the possibility of spectrophotometric determination of iron (III) for the analysis of real objects, model mixtures were compiled, the results of whic