PROSPEK SENYAWA FLAVONOID KULIT BATANG CEMPEDAK (Artocarpus champeden Spreng) SEBAGAI INHIBITOR DETOXIFIKASI HEME PARASIT MALARIA The detoxification inhibitory activity of heme malaria parasite by flavonoid in Cempedak bark (Artocarpus champeden Spreng)

Abstract

 ABSTRACTThe reaserch on the prospect of cempedak (Artocarpus champeden Spreng) bark as heme malaria parasite detoxificationinhibitor has been conducted. The flavonoid content was isolated from the methanolic extract of thecempedak bark and had been determine the anti-malaria propertise by in-vitro technique. The methode used wasBHIA (β- hematin Inhibitor Assay) and ultra observation of malaria parasite structure. The results showed thatisolates ME2 (IC50 = 0.35μg/ml) and morakhalkon compound A (IC50 = 0:28 mg /ml or 0.83μM) were isolatedfrom the methanol extract of the Cempedak bark is potential as an antimalarial. Compounds A and isolates morakhalkonME2 has a mechanism of action as an inhibitor of heme detoxification process of the malaria parasite.Therefore flavonoid from Cempedak bark is prospective developed as a new antimalarial drug. ABSTRAKPenelitian tentang prospek kulit batang cempedak (Artocarpus champeden Spreng) sebagai inhibitor detoxifikasiheme parasit malaria telah dilakukan. Senyawa flavonoid diisolasi dari ekstrak metanol kulit batang cempedakdan diuji potensi antimalarianya serta hambatan detoxifikasi heme secara in vitro. Pengujian hambatandetoxifikasi heme dilakukan dengan metode BHIA (β-hematin Inhibitor Assay) dan pengamatan ultra strukturparasit malaria. Hasil penelitian menunjukkan bahwa isolat ME2 (IC50=0.35μg/ml) dan senyawa morakhalkonA (IC50= 0.28 μg/ml atau 0.83μM) yang diisolasi dari ekstrak metanol kulit batang cempedak berpotensi sebagaiantimalaria. Senyawa morakhalkon A dan isolat ME2 memiliki mekanisme aksi sebagai inhibitor prosesdetoksifikasi heme parasit malaria. Oleh karena itu senyawa flavonoid dari kulit batang cempedak prospektifdikembangkan sebagai obat antimalaria baru.Kata kunci: Artocarpus champeden Spreng, antimalaria, inhibitor detoxifikasi heme