Butyratibacter algicola gen. nov., sp nov., a marine bacterium from the culture broth of Picochlorum sp 122

Abstract

A Gram-stain-negative, motile, non-spore-forming, ovoid or rod-shaped bacterium, designated strain B15(T), was isolated from the culture broth of a marine microalga, Picochlorum sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain B15(T) forms a stable cluster with Lutibaculum baratangense KCTC 22669(T) (95.4% 16S rRNA gene sequence similarity), Tepidamorphus gemmatus CB-27A(T) (94.9 %) and Microbaculum marinum HSF11(T) (94.6 %) in the family Rhodobiaceae. Optimal growth of strain B15(T) was observed at 33 degrees C, pH 8-9 and in the presence of 3% (w/v) NaCl. The only detected ubiquinone of strain B15(T) was Q-10, and the G+C content of the genomic DNA was 66.3 mol%. The major fatty acid profile comprised C-19 : 0 cyclo omega 8c, C-18 : 1 omega 7c/omega 6c and C-17 : 1 iso I/anteiso B. The major polar lipids of strain B15(T) were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, two unidentified aminolipids, and one unidentified lipid. Comprehensive analyses based on polyphasic characterization of strain B15(T) indicated that it represents a novel species of a new genusin the family Rhodobiaceae, for which the name Butyratibacter algicola gen. nov., sp. nov. is proposed. The type strain of the type species is B15(T) (= KCTC 52552(T) = MCCC 1K03221(T))