Using a microbiome for the dechlorination of 2,4-Dichlorophenol

Abstract

[[abstract]]本研究以微生物菌群（Microbiome）對2,4-二氯酚（2,4-DCP）進行脫氯，將Microbiome分為對數成長期（LM）與穩定期（SM）。對數成長期Microbiome對2,4-DCP無脫氯成效，穩定期180天Microbiome（SM180）降解對2,4-DCP有顯著脫氯成效，尤為50ppm最為顯著，氯離子釋出量為7ppm，氯離子釋出率為33%。Microbiome醱酵期間硫氮動態變化會促進對2,4-DCP脫氯，在SM60氯離子釋出不佳，SM90后開始氯離子釋出量提升。其中SM60~SM90氯離子釋放量有顯著提升，SM150有最佳氯離子釋出量為7ppm。LM與SM180氣體分析發現O2的減少與N2增加，這顯示在Microbiome混合液醱酵期間有脫氮功能微生物菌群的生成，導致有N2增加。從Gompertz方程式開發的簡單模型被應用於在10ppm~200ppm的2,4-DCP濃度與氯離子釋出速率的定量測量。2,4-DCP的濃度會影響Microbiome的微生物活性。回歸結果表明，在2,4-DCP濃度小於50ppm時，其氯離子釋出率、遲滯期相當。當大於50ppm濃度時氯離子釋出率與遲滯期隨著濃度的增加而降低，其中氯離子釋出率當2,4-DCP濃度為25ppm時，降低10%；2,4-DCP為75ppm時，降低50%。2,4-DCP濃度為300ppm時，微生物失去活性。在2,4-DCP濃度高於50ppm時，觀察到顯著的抑制。關鍵字：生物菌群、2,4-二氯酚、脫氯[[abstract]]Inthisstudy,2,4-dichlorophenol(2,4-DCP)wasdechlorinatedwithMicrobiome,andMicrobiomewasdividedintoLogarithmicphase（LM）andStationaryphase（SM）.LogarithmicphaseofMicrobiomeon2,4-dichlorophenolwithoutdechlorinationeffect，Stationaryphaseto180daysMicrobiome(SM180)degradationof2,4-DCPsignificantdechlorinationeffect，andtheconcentrationof2,4-dichlorophenolwas50ppm,thereleaseofchlorideionwas7ppm,andreleaseratewas33%.ThedynamicchangeofsulfurandnitrogenduringthefermentationofMicrobiomewillpromotethedechlorinationof2,4-DCP.IntheSM60Chlorideionsreleaseispoor,butSM90begantoincreasethereleaseofchlorideions.WhenSM60~SM90Chlorideionsreleasesignificantlyincreased,SM150hasthebestchlorideionreleaseof7ppm.LMandSM180gasanalysisrevealedanincreaseinN2,whichshowedthatduringthemicrobiomefermentationtherewasadenitrificationofthemicrobialflorathatresultedinanincreaseinN2.AsimplemodeldevelopedfromtheGompertzequationwasappliedtoquantitativemeasurementsoftheconcentrationof2,4-DCPat10ppmto200ppmwithchloridereleaserate.Theconcentrationof2,4-DCPaffectsmicrobiologicalactivityofMicrobiome.Theresultsindicatethatwhentheconcentrationof2,4-DCPislessthan50ppm,thechloridereleaserateandlagphaseareequivalent.Whentheconcentrationisgreaterthan50ppm,thechloridereleaserateandthelagphasedecreasewiththeincreaseoftheconcentration,andthechlorideionreleaserateis10%whenthe2,4-DCPconcentrationis25ppm;whenthe2,4-DCPis75ppm,Adecreaseof50%.2,4-DCPconcentrationof300ppm,themicrobiallossofactivity.Significantinhibitionwasobservedwhenthe2,4-DCPconcentrationwashigherthan50ppm.Keyword：Microbiome,2,4-dichlorophenol,dechlorination