A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria

McKerrow, J D; Vagg, S; McKinney, T; Seviour, E M; Maszennan, A M; Brooks, P R; Seviour, R J

journal article

oai:research.usc.edu.au:usc:1621

A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria

Authors: J D McKerrow
S Vagg
T McKinney
E M Seviour
A M Maszennan
P R Brooks
R J Seviour
Publication date: 1 January 2000
Publisher: 'Wiley'
Doi

Abstract

A simple and sensitive method for separating and detecting the LL, DD and meso diastereomers of the dibasic amino acid diaminopimelic acid (DAP) in the peptidoglycan of Gram-positive bacteria is described. This method is based on reverse phase HPLC separation of chiral derivatives of DAP followed by fluorescence detection of the o-phthaldehyde derivatives. Its application to the analyses of cell walls of several Gram-positive bacteria is described, where 10 mg or less of dry cells is required

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USC Research Bank - University of the Sunshine Coast

oai:research.usc.edu.au:usc:16...

Last time updated on 18/10/2017

This paper was published in USC Research Bank - University of the Sunshine Coast.

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