Background. Our study was designed to elucidate whether there were
miRNA and mRNA aberrantly expression profiles and potential role in malignant transformation of
16HBE induced by Cd. Methods. mRNA and miRNA expression profiles were determined
in 35th Cd-induced 16HBE and untreated 16HBE by microarray. A series of bioinformatics analyses
such as predicting targets, GO, KEGG were performed to find DEGs, coexpressing networks between
miRNAs and mRNAs and its functions. Results. 498 DEGs were found. 8 Cd-responsive
novel miRNAs predicted previously were identified, and 5 of them were downregulated. 214 target
genes were predicted for the Cd-responsive miRNAs, many of which appeared to regulate gene networks.
Target gene CCM2 was showed reciprocal effect by miRNAs. According to the combination analysis,
hsa-miR-27b-3p regulated most of the mRNAs, especially upregulated expression genes. The differentially
expressed miRNAs are involved in the biological processes and channels, and these GO and KEGG
enrichment analyses result were significantly enriched in the Cd-responsive. Discussion. These
results provided a tight link for the miRNA-mRNA integrated network and implied the role of novel miRNAs
in malignant transformation of 16HBE induced by Cadmium. It is better to understand the novel
molecular mechanism of cadmium-induced tumorigenesis
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