Metabolites of 2,3-diketogulonate delay peroxidase action and induce non-enzymic H2O2 generation : Potential roles in the plant cell wall
Abstract
A proportion of the plant's L-ascorbate (vitamin C) occurs in the apoplast, where it and its metabolitesmay act as pro-oxidants and anti-oxidants. One ascorbate metabolite is 2,3-dilcetogulonate (DKG), preparations of which can non-enzymically generate H2O2 and delay peroxidase action on aromatic substrates. As DKG itself generates several by-products, we characterised these and their ability to generate H2O2 and delay peroxidase action. DKG preparations rapidly produced a by-product, compound (1), with lambda(max) 271 and 251 nm at neutral and acidic pH respectively. On HPLC, (1) co-eluted with the major H2O2-generating and peroxidase-delaying principle. Compound (1) was slowly destroyed by ascorbate oxidase, and was less stable at pH 6 than at pH 1. Electrophoresis of an HPLC-enriched preparation of (1) suggested a strongly acidic (pK(a) approximate to 2.3) compound. Mass spectrometry suggested that un-ionised (1) has the formula C6H6O5, i.e. it is a reduction product of DKG (C6H8O7). In conclusion, compound (1) is the major H2O2-generating, peroxidase-delaying principle formed non-enzymically from DKG in the pathway ascorbate -> dehydroascorbic acid -> DKG -> (1). We hypothesise that (1) generates apoplastic H2O2 (and consequently hydroxyl radicals) and delays cell-wall crosslinking - both these effects favouring wall loosening, and possibly playing a role in pathogen defence. (C) 2017 The Authors. Published by Elsevier Inc.Peer reviewe- Article
- publishedVersion
- Ascorbate
- Dehydroascorbic acid
- Diketogulonate
- Apoplast
- Peroxidase
- Hydrogen peroxide
- Hydroxyl radical
- Plant cell wall
- L-ASCORBIC-ACID
- PERFORMANCE LIQUID-CHROMATOGRAPHY
- VITAMIN-C DEGRADATION
- DEHYDROASCORBIC ACID
- APOPLASTIC ASCORBATE
- HYDROXYL RADICALS
- 2,3-DIKETO-L-GULONIC ACID
- TRANSFORMATION PRODUCTS
- OXIDATIVE-DEGRADATION
- ARABIDOPSIS-THALIANA
- 1183 Plant biology, microbiology, virology
- 415 Other agricultural sciences