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Osmostress-induced changes in yeast gene expression

By J. Varela, Catelijne Van Beekvelt, Rudi Planta and Peter Mager

Abstract

When Saccharomyces cerevisiae cells are exposed to high concentrations of NaCI, they show reduced viability, methionine uptake and protein biosynthesis. Cells can acquire tolerance against a severe salt shock (up to 1.4 M NaCI) by a previous treatment with 0.7 M NaCI, but not by a previous heat shock. Two-dimensional analysis of [3H]-leucine-labelled proteins from salt-shocked cells (0.7 M NaCt) revealed the elevated rate of synthesis of nine proteins, among which were the heat-shock proteins hsp12 and hsp26. Northern analysis using gene-specific probes confirmed the identity of the latter proteins and, in addition, demonstrated the induction of glycerol-3-phos-phate dehydrogenase gene expression. The synthesis of the same set of proteins is induced or enhanced upon exposure of cells to 0.8 M sucrose, although not as dramatically as in an iso-osmolar NaCI concentration (0.7 M).FCT (formerly known as JNICT)

Topics: Yeast, Osmotic stress, Salt stress, Heat shock proteins
Publisher: Wiley
Year: 1992
DOI identifier: 10.1111/j.1365-2958.1992.tb01392.x
OAI identifier: oai:sapientia.ualg.pt:10400.1/4831
Provided by: Sapientia
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