Nematotoxic Phenolic Compounds from Melia azedarach Against Meloidogyne incognita.

Abstract

In the present study it was evaluated the paralysis activity, of the whole Italian and Algerian Melia azedarach fruits and parts (seeds, wood, kernel), against Meloidogyne incognita second stage juveniles (J2). Paralysis activity was evaluated in vitro after one hour and one day immersion periods of nematodes in test solutions. Phenolic composition of the extracts was elucidated by means of HPLC-DAD and quantification by HPLC-MS/MS. The water extract of the Italian M. azedarach fruit pulp (IPWE) showed significant nematicidal activity (EC50/48h=955 μg/mL) and among its active ingredient components were p-coumaric acid and p-hydroxybenzoic acid (EC50/48h = 840 and 871 μg/mL, respectively). This is the first report of the nematicidal activity of M. azedarach pulp water extract and phenolic acids against the root knot nematode M. incognita