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Distinct sources of hematopoietic progenitors emerge before HSCs and provide functional blood cells in the mammalian embryo

By Kathleen E. McGrath, Jenna M. Frame, Katherine H. Fegan, James R. Bowen, Simon J. Conway, Seana C. Catherman, Paul D. Kingsley, Anne D. Koniski and James Palis


Hematopoietic potential arises in mammalian embryos before adult-repopulating hematopoietic stem cells (HSCs). At E9.5, we show the first murine definitive erythro-myeloid progenitors (EMPs) have an immunophenotype distinct from primitive hematopoietic progenitors, maturing megakaryocytes and macrophages, and rare B cell potential. EMPs emerge in the yolk sac with erythroid and broad myeloid, but not lymphoid, potential. EMPs migrate to the fetal liver and rapidly differentiate including production of circulating neutrophils by E11.5. While the surface markers, transcription factors and lineage potential associated with EMPs overlap with those found in adult definitive hematopoiesis, they are present in unique combinations or proportions that result in a specialized definitive embryonic progenitor. Further, we find that ES cell -derived hematopoiesis recapitulates early yolk sac hematopoiesis, including primitive, EMP and rare B cell potential. EMPs do not have long term potential when transplanted in immunocompromised adults, but can provide transient adult-like RBC reconstitution

Topics: Hematopoietic stem cells, Hematopoiesis, Megakaryocytes, Macrophages, Erythro-myeloid progenitors, embryonic stem cell
Publisher: Elsevier B.V.
Year: 2015
DOI identifier: 10.1016/j.celrep.2015.05.036
OAI identifier:
Provided by: IUPUIScholarWorks

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