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Development and application of evanescent wave cavity ring-down spectroscopy as a probe of biologically relevant interfaces

By Hayley Victoria Powell


The application of a hybrid instrument combining Evanescent Wave Cavity Ring-Down Spectroscopy (EW-CRDS) with electrochemical and fluidic methods is described. The electrochemical/fluidic methods were used to induce a surface process, the effects of which were subsequently monitored in situ and in real time with exquisite spectral sensitivity and excellent temporal resolution by EW-CRDS. The well-defined manner in which the surface processes were initiated allowed the extraction of kinetic rate constants by fitting the EW-CRDS data to mathematical models of the surface process coupled to convection-diffusion.\ud The investigations described include: the study of the thermodynamics and kinetics of the adsorption of tris(bipyridine)ruthenium(II) ([Ru(bpy)3]2+) to polypeptide films using EW-CRDS with chronoamperometry; the real-time electrochemistry of cytochrome c immobilised on silica by EW-CRDS with chronoamperometry; the kinetics of adsorption and DNA-assisted desorption of 5,10,15,20-tetra(N-methylpyridinium-4-yl)porphyrin at the silica-water interface using EW-CRDS with an impinging jet flow cell; and the monitoring the adsorption of cationic phospholipid vesicles at the silica-aqueous interface and the interaction of 5,10,15,20-Tetraphenyl-21H, 23H-porphine-p,p′,p″,p′′′-tetrasulfonic acid tetrasodium hydrate with the resulting bilayer also using EW-CRDS with an impinging jet flow cell.\ud The work described in this thesis provides a platform on which EW-CRDS can be used to study dynamics at biointerfaces, such as the association of ions, peptides, proteins and drugs with phospholipid bilayers, the electron transfer between redox enzymes in a biomimetic environment, and the lateral diffusion of protons, ions and proteins at biomembranes. Such studies are essential to the understanding of many important cellular processes in addition to the development and optimisation of a number of bio-inspired technologies

Topics: QD, QH301
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  3. (2005). AFM characterization of solid-supported lipid multilayers 181 prepared by spin-coating. Biochimica Et Biophysica Acta-Biomembranes doi
  4. (1992). Bilayer lipid-membranes - an experimental system for biomolecular electronic devices development. doi
  5. (1992). Biomembranes as catalysts of mass, energy and information-transfer. doi
  6. (2009). Capturing the nanoscale complexity of cellular membranes in supported lipid bilayers. doi
  7. (2008). Cationic liposomal lipids: From gene carriers to cell signaling. doi
  8. (2007). Characterization of solid supported lipoplexes by FTIR microspectroscopy. doi
  9. (2007). Characterization of Two Types of Silanol Groups on Fused-Silica Surfaces Using Evanescent-Wave Cavity Ring-Down doi
  10. (2001). Comments on rupture of absorbed vesicles. Langmuir doi
  11. (2007). Comparison of liposomes formed by sonication and extrusion: Rotational and translational diffusion of an embedded chromophore. Langmuir doi
  12. (2005). Conducting polymer polypyrrole supported bilayer lipid membranes. doi
  13. (2006). Controlling the pathway of formation of supported lipid bilayers of DMPC by varying the sodium chloride concentration. Thin Solid Films doi
  14. (1997). Conversion of light energy to proton potential in liposomes by artificial photosynthetic reaction centres. Nature doi
  15. (1999). Cyclic transmembrane charge transport by pyrylium ions in a vesicle-based photocatalytic system. Nature doi
  16. (2005). Dissipation-Enhanced Quartz Crystal Microbalance Studies on the Experimental Parameters Controlling the Formation of Supported Lipid Bilayers. doi
  17. (1997). Electrochemistry of supported bilayer lipid membranes: Background and techniques for biosensor development. Bioelectrochemistry and Bioenergetics doi
  18. Electroreduction of oxygen by cytochrome c oxidase immobilized in electrode-supported lipid bilayer membranes. doi
  19. (2006). Electrostatic stitching in gel-phase supported phospholipid bilayers. doi
  20. (2005). Following the formation of supported lipid bilayers on mica: A study combining AFM, QCM-D, and ellipsometry. doi
  21. (2008). Formation and Characterization of Fluid Lipid Bilayers on Alumina. Langmuir doi
  22. (2006). Formation of solid-supported lipid bilayers: An integrated view. Langmuir doi
  23. (2009). Formation of Supported Bilayers on Silica Substrates. Langmuir doi
  24. (2000). Formation of supported membranes from vesicles. Physical Review Letters doi
  25. (2004). In situ investigations of the formation of mixed supported lipid bilayers close to the phase transition temperature. Nano Letters doi
  26. (2009). Influence of Mono- And Divalent Ions on the Formation of Supported Phospholipid Bilayers via Vesicle Adsorption. Langmuir doi
  27. (2008). Lipid bilayer structure determined by the simultaneous analysis of neutron and x-ray scattering data. doi
  28. (1994). Lipid transfer between small unilamellar vesicles and single bilayers on a solid support - self-assembly of supported bilayers with asymmetric lipid distribution. Biochemistry doi
  29. (1999). Liposome adhesion on mica surface studied by atomic force microscopy. Langmuir doi
  30. (2007). Mitochondrial ion channels. Annual Review of Physiology doi
  31. (2000). Mobile phospholipid bilayers on a polyion/alkylthiol layer pair. Langmuir doi
  32. (2008). Ordering and lyotropic behavior of a silicon-supported cationic and neutral lipid system studied by neutron reflectivity. Applied Physics Letters doi
  33. (2005). Particle size distribution in DMPC vesicles solutions undergoing different sonication times. doi
  34. (2003). Pathways of lipid vesicle deposition on solid surfaces: A combined QCM-D and AFM study. doi
  35. (2005). Patterned biomimetic membranes: Effect of concentration and pH. Langmuir doi
  36. (2006). Phase separation of lipid membranes analyzed with high-resolution secondary ion mass spectrometry. Science doi
  37. (1981). Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucoside. Biochemistry doi
  38. (1987). Planar artificial biomembranes optimized for biochemical assay. Analytica Chimica Acta doi
  39. (1985). Planar bilayer lipid-membranes. Progress in Surface Science doi
  40. (2009). Porous Nanoparticle Supported Lipid Bilayers (Protocells) as Delivery Vehicles. doi
  41. (2004). QCM-D on mica for parallel QCM-D-AFM studies. Langmuir doi
  42. (2005). Raman spectra of planar supported lipid bilayers. Biochimica Et Biophysica Acta-Biomembranes doi
  43. (1962). Reconstitution of cell membrane structure in vitro and its transformation into an excitable system. Nature doi
  44. (1997). Self-assembled bilayer lipid membranes: from mimicking biomembranes to practical applications. Bioelectrochemistry and Bioenergetics doi
  45. (2007). Self-assembled lamellar complexes of siRNA with lipidic aminoglycoside derivatives promote efficient siRNA delivery and interference. doi
  46. (2007). Sensitive probing of DNA binding to a cationic lipid monolayer. doi
  47. (1977). Simple Method for Preparation of Homogeneous Phospholipid Vesicles. Biochemistry doi
  48. (2006). Solid supported lipid bilayers: From biophysical studies to sensor design. Surface Science Reports doi
  49. (2006). Spectroscopic infrared near-field microscopy and x-ray reflectivity studies of order and clustering in lipid membranes. Applied Physics Letters doi
  50. (1998). Structural studies of polymer-cushioned lipid bilayers. doi
  51. (2006). Structure and mobility of lipid membranes on a thermoplastic substrate. Langmuir doi
  52. (2005). Structure of a gel phase lipid bilayer prepared by the Langmuir-Blodgett/Langmuir-Schaefer method characterized by sum-frequency vibrational spectroscopy. Langmuir doi
  53. (2007). Structure of anionic phospholipid coatings on silica by dissipative quartz crystal microbalance. Langmuir doi
  54. (2006). Supported lipid bilayer formation by the giant vesicle fusion induced by vesicle-surface electrostatic attractive interaction. Chemical Physics Letters doi
  55. (2004). Supported membranes with well-defined polymer tethers-incorporation of cell receptors. Chemphyschem doi
  56. (1986). Supported planar membranes in studies of cell-cell recognition in the immune-system. Biochimica Et Biophysica Acta doi
  57. (1998). Surface specific kinetics of lipid vesicle adsorption measured with a quartz crystal microbalance. doi
  58. (2004). Surfacedependent transitions during self-assembly of phospholipid membranes on mica, silica, and glass. Langmuir doi
  59. (2008). Tethered bimolecular lipid membranes - A novel model membrane platform. Electrochimica Acta doi
  60. (2002). Vesicle adsorption on SiO2 and TiO2: Dependence on vesicle size. doi
  61. (2004). X-ray and neutron reflectivity study of solid-supported lipid membranes prepared by spin coating. doi

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