Introduction: Biofilms
are population of bacteria cells that cause irreversible binding to the
surfaces by producing extracellular polymers. Biofilm formation in bacteria
causes resistance to antimicrobial agents and can lead to severe problems in
this ground. Materials and methods: The purpose of this study was to evaluate biofilm
formation in some isolates of Pseudomonas aeruginosa (13 case), Staphylococcus
aureus (13 case), Enterobacter (13 case) and Acinetobacter
(13 case) which were collected from human infections of Alzahra hospital in
Isfahan. Also the minimum inhibitory concentration of chlorhexidine and its
impact on the growth of planktonic and biofilm formation for these isolates
were determined. Statistical analysis and graphing have been carried out by
using SPSS software (version 20) and Excel. Results: All isolates (52 isolates) have produced biofilm. The
mean of MIC of chlorehexidine antiseptic for the p.aeruginosa, S.aureus,
Enterobacter and Acinetobacter were 0/001, 0/00013, 0/001, 0/0003
g/ml respectively. Planktonic bacterial growth inhibition from p.aeruginosa
and Enterobacter in 1/4 MIC and 1/8 MIC respectively was seen in 40 and
60 % cases. Acinetobacter and Staphylobacter aureus, have been
controlled in 40 % of cases in 1/4 MIC and 60 % of cases in 1/8 MIC. Biofilm
has not been produced in any of MIC and 2MIC dilution, and the power of biofilm
formation had been increased significantly by reducing concentration of
chlorhexidine dilution. Discussion and
conclusion: The results indicate that
the use of chlorhexidine in appropriate concentrations (MIC) can prevent
bacterial growth and biofilm formation in different species causing hospital
infections, but doses of chlorhexidine that are less than the MIC can stimulate
biofilm formation
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