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Co-occurrence analysis of microbial taxa in the Atlantic Ocean reveals high connectivity in the free-living bacterioplankton

By Mathias eMilici, Zhi-Luo eDeng1, Jürgen eTomasch, Johan eDecelle, Melissa eWos-Oxley, Hui eWang, Ruy eJáuregui, Iris ePlumeier, Helge-Ansgar eGiebel, Thomas eBadewien, Masha eWurst, Dietmar ePieper, Meinhard eSimon and Irene eWagner-Doebler

Abstract

We determined the taxonomic composition of the bacterioplankton of the epipelagic zone of the Atlantic Ocean along a latitudinal transect (51°S – 47°N) using Illumina sequencing of the V5-V6 region of the 16S rRNA gene and inferred co-occurrence networks. Bacterioplankon community composition was distinct for Longhurstian provinces and water depth. Free-living microbial communities (between 0.22-3 µm) were dominated by highly abundant and ubiquitous taxa with streamlined genomes (e.g. SAR11, SAR86, OM1, Prochlorococcus) and could clearly be separated from particle-associated communities which were dominated by Bacteroidetes, Planktomycetes, Verrucomicrobia and Roseobacters. From a total of 369 different communities we then inferred co-occurrence networks for each size fraction and depth layer of the plankton between bacteria and between bacteria and phototrophic micro-eukaryotes. The inferred networks showed a reduction of edges in the deepest layer of the photic zone. Networks comprised of free-living bacteria had a larger amount of connections per OTU when compared to the particle associated communities throughout the water column. Negative correlations accounted for roughly one third of the total edges in the free-living communities at all depths, while they decreased with depth in the particle associated communities where they amounted for roughly 10% of the total in the last part of the epipelagic zone. Co-occurrence networks of bacteria with phototrophic micro-eukaryotes were not taxon-specific, and dominated by mutual exclusion (~60%). The data show a high degree of specialization to micro-environments in the water column and highlight the importance of interdependencies particularly between free-living bacteria in the upper layers of the epipelagic zone

Topics: Atlantic Ocean, Network analysis, next generation sequencing, bacterioplankton, 16S rRNA analysis, Bacteria-bacteria interactions, Microbiology, QR1-502
Publisher: Frontiers Media S.A.
Year: 2016
DOI identifier: 10.3389/fmicb.2016.00649
OAI identifier: oai:doaj.org/article:d5e28b01efa24b7dba24dc68061d7ac4
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