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Stem cell ribonomics: RNA-binding proteins and gene networks in stem cell differentiation

By Patricia eShigunov and Bruno eDallagiovanna


Stem cells are undifferentiated cells with the ability to self-renew and the potential to differentiate into all body cell types. Stem cells follow a developmental genetic program and are able to respond to alterations in the environment through various signaling pathways. The mechanisms that control these processes involve the activation of transcription followed by a series of posttranscriptional events. These posttranscriptional steps are mediated by the interaction of RNA-binding proteins (RBPs) with defined subpopulations of RNAs creating a regulatory gene network. Characterizing these RNA-protein networks is essential to understanding the regulatory mechanisms underlying the control of stem cell fate. Ribonomics is the combination of classical biochemical purification protocols with the high-throughput identification of transcripts applied to the functional characterization of RNA-protein complexes. Here, we describe the different approaches that can be used in a ribonomic approach and how they have contributed to understanding the function of several RBPs with central roles in stem cell biology

Topics: RNA-Binding Proteins, Stem Cells, differentiation, gene networks, Ribonomics, Biology (General), QH301-705.5
Publisher: Frontiers Media S.A.
Year: 2015
DOI identifier: 10.3389/fmolb.2015.00074
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