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Molecular pathways of silica nanoparticle formation and biosilicification

By Dominique Jeanette Tobler


Biosilicification and silica nanoparticle formation occur in many modem terrestrial environments and they also played an important role in ancient geological settings. This thesis presents results from (i) field studies in Icelandic geothermal waters that aimed at quantifying the parameters that control the growth rate and texture of sinters and the diversity and silicification of associated microbial communities and (ii) lab studies that focussed on the\ud kinetics and mechanisms of silica nanoparticle forination under conditions mimicking natural geothermal environments.\ud \ud The analysis of growth rates and textures of sinters from five geochemically very different Icelandic geothermal areas showed that the inorganic silica precipitation rate was strongly influenced by temperature, pH, ionic strength, and silica concentration. In addition, the presence\ud of thick biofilms seemed to have aided the precipitation process by simply providing "sticky" surfaces. In turn, the structural and textural development of sinters was affected by the precipitation rate and mechanism (subaqueously and/or subaerially) as well as the presence and\ud absence of microbial communities. As a result, porous, subaequeouss inters developed at sites with medium to high sinter growth rates and low microbial activity. Conversely, dense, heterogeneouss inters formed in geothermal waters characterized by low precipitation rates and extensive biofilms. With time these biofilms became fully silicified and well preserved within the sinter edifices. The diversity of microbial communities in hot spring environments appeared to be directly controlled by the physico-chernical conditions of the geothermal waters (i. e., T,pH, salinity and sinter growth rate) and the most dominant phylotypes were related to Aquificae, Deinococci and y-Proteobacteria.\ud \ud The rates and mechanisms of the initial steps of silica polymerisation and silica nanoparticle formation were quantified in-situ and time-resolved using synchrotron-based small angle x-ray scattering (SAXS). The experiments were carried out in near neutral pH solutions with initial\ud Si02 between 640 - 1600 ppm, ionic strength of 0.02 - 0.22 M, and added organics (glucose, glutarnic acid, xanthan gum). The polymerization reactions were induced either by neutralising a high pH solution or by rapid cooling of a supersaturatedh ot silica solution. From the analysis\ud of the time-resolved SAXS data, a kinetic model for the nucleation and growth of silica nanoparticles was derived suggesting a 3-stage process: (1) homogeneous nucleation of critical nuclei (I -2 run; depending on the concentration regimes), (2) 3-dimensional, surface-controlled particle growth following 1st order reaction kinetics and (3) Ostwald ripening and particle aggregation. At the end of this 3-stage process, regardless of the tested silica concentration, ionic strength or added organics, the final particle diameter was about 8nm characterised by open, polymeric (i. e., mass fractal) structures. The kinetics of particle growth were unaffected by the two different methods to induce silica polymerisation (pH-drop vs. T-drop) however, the growth processes proceeded substantially slower if silica polymerisation was induced by fast\ud cooling as opposed to pH-drop. In contrast, the addition of organics did not affect the reaction rates.\ud \ud The nucleation and growth of silica nanoparticles under constant re-supply Of fresh silica solution (i. e., hot springs) was simulated using a flow-through geothermal simulator system. The effect of silica concentration ([Si02D, ionic strength (IS), temperature and organic additives on the size and polydispersity of silica nanoparticles was quantified. VVhile the applied increase\ud in IS did not affect the size (30 - 35 nm) and polydispersity (± 9 nm) observed at 58 C, an increase in [Si02] notably enhanced silica polymerisation and also resulted in slightly smaller particle sizes. The biggest effect was observed with a decrease in temperature (58 to 33 C) or the addition of glucose: in both cases particle growth was restricted to sizes below 20 mn. Conversely, the addition of xanthan gum induced the development of a thin silica-rich film that enhanced silica aggregation

Publisher: School of Earth and Environment (Leeds)
Year: 2008
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  1. 1) Growth and ripening of silica polyiners in aqueous solutions. doi
  2. 1) Phosphorus Metabolism, vol. I, Johns Hopkins doi
  3. (2000). 213) Zýý 600 -CL 500 -'0' 100 1 amorphous 20 40 60 Time (min) --o-inorganic 300ppm gkicose 300ppm gkitamic acid 150ppm xarthan gum and Amorsson,
  4. (1961). 7he Scientific Papers, VoL L
  5. (1997). A chemical equilibrium model for metal adsorption onto bacterial surfaces. doi
  6. (2000). A molecular comparison of culturable aerobi heterotrophic bacteria and 16S rDNA clones derived from a deep subsurface sediment. doi
  7. (1997). A Molecular View doi
  8. (1989). A review on solubility and polymerisation of silica. doi
  9. (1937). A statistical theory for the recrystallization of metals.
  10. (1979). A theoretical model of the polymerization of silica in aqueous media. doi
  11. (2005). Abiotic-biotic controls on the origin and development of spicular sinter: in situ growth experiments, doi
  12. (1999). Actively growing siliceous oncoids in the Waiotapu geothermal area, North Island, New Zealand. doi
  13. (1996). Actualistic taphonomy of cyanobacteria: implications for Precambrian fossil record.
  14. (2006). Alteration of sinter diagenesis in an active furnarole, Taupo volcanic zone, New Zealand. doi
  15. American Institute ofMining and Engineering,
  16. (1998). Amorphous silica precipitation (60 to 120 degrees Q: Comparison of laboratory and field rates. doi
  17. (1980). Amorphous silica solubilities - II. Effect of aqueous salt solutions at 25"C. Geochimica et Cosmochimica Acta, doi
  18. (1982). Amorphous silica solubilities-V. Predictions of solubility behaviour in aqueous mixed electrolyte solutions to 3000C. Geochimica et Cosmochimica Acta, doi
  19. (2000). Amorphous silica solubility and the thermodynamic properties Of I14SiO4 in the range of 0' to 350'C at P,,,,. Geochimica et Cosmochimica Acta, doi
  20. (1997). Anaerobic microbiology of an alkaline Icelandic hot spring. doi
  21. (1992). Analysis of zeolite crystallizations using Avrami transformation methods. doi
  22. and Radosavljcvi6-Evans 1. (2004) Beyond classical applications of powder diffraction. doi
  23. (2005). Archaeal and bacterial communities in geochernically diverse hot springs of Yellowstone National Park USA. doi
  24. (2007). Archaeal diversity in Icelandic hot springs. doi
  25. (1988). Basic principles of colloid science. Royal Society of Chemistry Paperbacks, doi
  26. (1997). Biodiversity within hot springs microbial communities: molecular monitoring of enrichment cultures. Antoine Van Leeuwenhoek,
  27. (1997). Biogenicity of silica precipitation around geysers and hot spring vents, North Island, New Zealand. doi
  28. (2001). Biomineralization in New Zealand Geothermal Areas.
  29. (2003). Biosilicification: structure, regulation of structure and model studies. In: Silicon chemistry -from molecules to *extended systems doi
  30. (2005). Biosilicification: the role of cyanobacteria in silica sinter deposition, In Micro-organisms and earth systems: advances in geomicrobiology doi
  31. (2000). Biosilicification: the role of the organic matrix structure control. doi
  32. (1989). Brine classification at Svartsengi, Iceland: Effect of pH and temperature on the precipitation of silica and its properties. doi
  33. (1997). Brock Biology ofMicroorganisms, Prentice Hall International, 8th edition.
  34. (1999). Calorimeteric and highresolution transmission electron microscopy study of nanocrystallization in zirconia gel. doi
  35. (1990). Chemical Factors influencing the rated and sequences of silica phase transitions: Effects of organic constituents. doi
  36. (1973). Colloidal silica.
  37. (1956). Colorimetric method for detennination of sugars and related substances. doi
  38. (2001). Community structure along a thermal gradient in a stream near Obsidian Pool, Yellowstone National Park. doi
  39. (2003). Comparison of the acid-base behaviour and metal adsorption characteristics of a Gram-negative bacterium with other stmins. doi
  40. (2006). Composition and implications of diverse lipids in New Zealand Geothermal sinters. doi
  41. (1980). Condensation of silica from supersaturated silicic acid solutions. doi
  42. (1968). Controlled growth of monodisperse silica spheres in the micron size range. doi
  43. (1972). CRC Handbook of Chemistry and Physics, 53d edition, Chemical Rubber Co. Weres 0.,
  44. (2000). Cyanobacterial viability during hydrothermal. biomineralisation. doi
  45. (1998). Deducing growth mechanisms for minerals from the shapes of crystal size distributions. doi
  46. (1992). Determination of the regularization parameter in indirect-transform methods using perceptual criteria. doi
  47. (1991). Diagensis of siliceous particles in subAntarctic sediments, ODP Leg 114, Hole 699A: possible microbial mediation. doi
  48. (1956). Dissolution and prccipitation. of silica at low temperaturcs.
  49. (1972). Dissolution of opal in water and its water content. doi
  50. (2001). Distribution kinetics theory of Ostwald ripening. doi
  51. (1983). Distribution of Thermus spp. in Icelandic hot springs and a therinal gradient. doi
  52. (1996). Diversity of iron and silica precipitation by microbial mats in hydrothermal waters, Iceland: Implications for Precambrian iron formations. doi
  53. (1995). DNA relatedness of Thermus strains, description of Viermus brockianus sp. nov., and proposal to reestablish Thermus thermophilus (Oshima and Imahori), doi
  54. (2001). DNA sequencing protocols. doi
  55. (1988). Dynamics of Growth of Silica Particles from AmmoniaCatalyzed Hydrolysis of Tetra-ethyl-orthosilicate. doi
  56. (1995). Ecology, distribution and isolation of Thermus. doi
  57. (1956). Effect of p1l on polymerization of silicic acid. doi
  58. (1979). Effect of silica polymerisation and pH on geothennal scaling. doi
  59. (1977). Effect of temperature and concentration on the rate of polymerization of silica in geothermal waters.
  60. (2007). Evidence for a possible siliceous sinter deposit at Home Plate in Gusev Crater.
  61. (1985). Exceptional preservation of photosynthetic organisms in silicified carbonates and silicified peats. doi
  62. (2003). Experimental studies on New Zealand hot spring sinters: rates of growth and textural development. doi
  63. (2003). Experimental study of iron and silica immobilization by bacteria in mixed Fe-Si systems: implications for microbial silicification in hot springs. doi
  64. (2000). Experimental study of the pH-, ionic strength-, and reversibility behaviour of bacteria-mineral adsorption. doi
  65. (2002). Factors governing subaqueous siliceous sinter precipitation in hot springs: examples from Yellowstone National Park, doi
  66. (1990). Fast aggregation of colloidal silica. Physical Review A, 41,4379-439 1. 241 Makrides doi
  67. (2000). Feldspar saturation state in natural waters.
  68. (2005). Formation mechanism and morphology in precipitation of vateritenano-aggregation or crystal growth? doi
  69. (1992). Formation of colloidal silica particles from alkoxides. doi
  70. (1994). Formation of fine-grained metal and silicate precipitates on a bacterial surface (Bacillus subtilis). doi
  71. (1996). Fossilization processes in siliceous thermal springs trends in preservation along thermal gradient. doi
  72. (2006). Gene cloning and DNA analysis: An introduction. 5th edition, doi
  73. (1992). Geochemical model to calculate speciation of major, trace and redox elements in natural waters,
  74. (1998). Growth kinetics of nanocrystalline ZnO particles from colloidal suspensions. doi
  75. (2007). Horizontal Gene Transfer and Homologous Recombination Drive the Evolution of the Nitrogen-Fixing Symbionts of Medicago Species. doi
  76. (2003). Hot spring sinters: keys to understanding Earth's earliest life forms. doi
  77. (1976). Hot-spring sediments in Yellowstone National Park. In: doi
  78. (2005). Hydrogen and bioenergetics in the Yellowstone geothermal ecosystem. doi
  79. (1976). Hydrothennal crystallization of silica gel. doi
  80. (1995). In situ silicification of an Icelandic hot spring microbial mat: Implications for microfossil formation. doi
  81. (2000). Influence of sulfide and temperature on species composition and community structure of hot spring microbial mats. doi
  82. (1996). Influence of thermophilic bacteria on calcite and silica precipitation in hot springs with water temperatures above 90C: Evidence from Kenya and New Zealand. doi
  83. (1986). Iron-silica crystallite nucleation by bacteria in a geothermal sediment. doi
  84. (1993). Kinetics of crystal growth in a terrestrial magma ocean. doi
  85. (1940). Kinetics of phase change,
  86. (2005). Kinetics of silica oligornerization and nanocolloid formation as a function of pH and ionic strength at 25T. Geochimica et Cosmochimica Acta, doi
  87. (1979). Kinetics of silica polymerisation and deposition from dilute solutions between 5 and 180'C. doi
  88. (1993). Kinetics of the amorphous-tonanocrystalline transfonnation in Fe73.5CU, Nb3Sil3.5B9.
  89. (1939). La diffraction des rayons X aux tres petits angles: application al'6tude de ph6nomenes Ultramicroscopiques. Annals ofPhysics,
  90. (1990). Light Scattering of Silica Particles in Solution. doi
  91. (1984). Limits of the Fractal Dimension for Irreversible Kinetic Aggregation of Gold Colloids. Physical Review Letters, doi
  92. (2005). Lipid biomolecules in silica sinters: indicators of microbial biodiversity. doi
  93. (1978). Major element chemistry of geothermal seawater at Reykjanes and Svartsengi, doi
  94. (2002). Manual ofEnvironmental Microbiology.
  95. (1975). Metamorphism and Metamorphic Bells, doi
  96. (1998). Microbial biofacies in hot springs sinters: a model based on Ohaaki pool, North Island, New Zealand. doi
  97. (2008). Microbial biomass: A catalyst for CaC03 precipitation in advection-dominated transport regimes. doi
  98. (2002). Microbial composition of near-boiling silica depositing thermal springs throughout Yellowstone National Park. Applied Environmental Mircobiology, doi
  99. (2001). Microbial construction of siliceous stalactites at geysers and hot springs: examples from Whakarewarewa geothermal area, North Island, New Zealand. doi
  100. (2000). Microbial diversity at 83 degrees C doi
  101. (1997). Microbial participation in the formation of siliceous deposits from geothermal water and analysis of the extremely thermophilic bacterial community. doi
  102. (2003). Microbial silica deposition in geothennal hot waters. doi
  103. (2004). Microbial silicification in Iodine Pool, Waimangu geothermal area, North Island, New Zealand: implications for recognition and identification of ancient silicified microbes. doi
  104. (2001). Microbial-biomineral interactions and their significance for the formation of chemical sediments.
  105. (2001). Microbialsilica interactions in Icelandic hot springs sinter: possible analogues for some Precambrian siliceous stromatolites. doi
  106. (2007). Microfacies of strornatolitic sinter from acid-sulphate-chloride springs at Parariki Stream. Rotokawa geothermal field, New Zealand.
  107. (1965). Microorganisms from the gunflint chert. doi
  108. (1996). Mineralization of bacterial surfaces, doi
  109. (2000). Mineralogical and textural changes accompanying ageing of silica sinter. doi
  110. (2007). Modeling the kinetics of silic 'a nanocolloid fonnation and growth in aqueous solutions as a function of pH and ionic strength. doi
  111. (1969). Models for Solid-State Reactions in Powered Compacts: A Review.
  112. (2004). Molecular characterization of cyanobacterial silicification using synchrotron infrared micro-spe'ctroscopy. doi
  113. (1996). Nanocrystalline metals crystallized from amorphous solids: nanocrystallization, structure, and properties. doi
  114. (1974). Near infrared adsorption spectra (4000-9000 crif 1) of opals and the role of water
  115. (2000). New controlled environment vitrification system for cryo-transmission electron microscopy: design and application to siufactant solutions. doi
  116. (2002). New isolates and physiological properties of the Aquificales and description of Thermocrinis albus sp. doi
  117. (1998). Novel division level bacteria diversity in a Yellowstone hot spring.
  118. (1995). Novel routes to designer silicas: studies of the decomposition Of (M+)2[Si(C611402)31 -xH20. Importance of M+ identity of the kinetics of oligornerisation and the structural characteristics of the silicas produced. doi
  119. (2003). Opal-A and associated microbes from Wairakei, New Zealand: the first 300 days. doi
  120. (1988). Ostwald ripening in a system with a high, volume fraction of coarsening phase. doi
  121. (2003). Partitioning of bacterial communities between travertine depositional facies at Mammoth Hot Springs, Yellowstone National Park, doi
  122. (1999). Photosynthetic controls on the silicification of cyanobacteria.
  123. (1994). Phylogenetic analysis of the hyperthermophilic pink filament community in Octopus Spring, Yellowstone National Park. Applied and Environmental Microbiology,
  124. (2001). Phylogenetic characterisation of the blue filamentous bacterial community from and Icelandic geothermal spring. doi
  125. (2002). Phylogenetic characterization of microbial mats and streamers from a Japanese alkaline hot springs with a thermal gradient. doi
  126. (1998). Phylogenetic evidence for the existence of novel thertnophilic bacteria in hot spring sulphur-turf rnicrobial mats in Japan. Applied and Environment Microbiology,
  127. (1995). Phylogenetic identification and in situ detection of individual microbial cells without cultivation. doi
  128. (2000). Polydispersity during formation and growth of the St6ber silica particles from Small-Angle X-Ray Scattering measurements. doi
  129. (1995). Polymer physics. London, doi
  130. (1959). Polymerisation und Depolymerisation der Kieselsäure unter verschiedenen Bedingungen. doi
  131. (1978). Precipitation of calcite from flashed geothermal waters in Iceland. Contributions Mineralogical Petrology, doi
  132. (1996). Primary silica oncoids from Orakeikorako hot springs, North Island, New Zealand.
  133. (1997). Probing the dynamics of the silica nanostructure fonnation and growth by SAXS. Chemistry ofMaterials, doi
  134. (1991). Quartz dissolution in organic-rich aqueous systems. doi
  135. (1939). Reaction kinetics in processes of nucleation and growth.
  136. (2007). Recent results from the Spirit Rover at Home Plate and " Silica Valley". Eos Transactions,
  137. (2001). Relationship between spring and geyser activity and the deposition and morphology of high temperature (>73*C) siliceous sinter, Yellowstone National Park, doi
  138. (1994). Remarkable archaeal diversity detected in a Yellowstone National Park hot spring enviromnent. doi
  139. (2001). Role of biornineralization as an ultraviolet shield: Implications for Archean life. doi
  140. (1997). Scanning Transmission X-Ray Microscopy; a new method for the investigation of aggregation in silica. doi
  141. (1996). Seasonal changes in silica deposition in hot spring systems. doi
  142. (1985). Silica diagenesis, IL General mechanisms.
  143. (1991). Silica gel from water glass: a SAXS study of the formation and ageing of fractal aggregates. doi
  144. (1956). Silica in hot spring waters. doi
  145. (2001). Silica precipitating peptides from Diatoms: The chemical structure of silaffm-lA from Cylindrotheca fusiformis. doi
  146. (2003). Silica scaling: the main obstacles in efficient use of high-temperature geothermal fluids.
  147. (1972). Siliceous algal and bacterial stromatolites in hot spring and geyser effluents of Yellowstone National Park, doi
  148. (1991). Silicification of fossils. doi
  149. (1976). Silicification of wood.
  150. (2003). Silicified microbes in a geyser mound: the enigma of low-temperaturc cyanobacteria in a Mgh-temperature setting. doi
  151. (2003). Size, volume fraction, and nucleation of St6ber silica nanoparticles. doi
  152. (1982). Small Angle X-ray Scattering. doi
  153. (2003). Small-angle scattering studies of biological macromolecules in solution. doi
  154. (1998). Solution chemistry of wood silicification.
  155. (1984). Solution to the multiparticle diffusion problem with applications to Ostwald ripening. doi
  156. (2001). Species composition of cultivated and noncultivated. bacteria from short filaments in an Icelandic hot spring at 8 8C.
  157. (1995). Spherulites in Calcrete laminar crusts - biogenic CaC03 precipitation as a major contributor to crust formation. doi
  158. (1985). Standard Methods for the Examination of Water and Wastewater. American Public Health Association,
  159. (1994). Structure-function relationships in microbial exopoly-saccharides.
  160. (2008). Sul(urihydrogenibium krisyansson! sp. nov., a hydrogen- and sulfur-oxidizing thermophile isolated from a terrestrial Icelandic hot spring. doi
  161. (1985). The behaviour of silica in hydrothermal solutions.
  162. (1983). The chemistry of geothermal waters in Iceland. Il Mineral equilibria and independent variables controlling water compositions. doi
  163. (1983). The chemistry of geothermal waters in Iceland. Ill. Chemical geothermometry in geothermal investigations. doi
  164. (1982). The chemistry of iron in geothermal systems doi
  165. (1979). The chemistry ofsilica,
  166. The comparison to the inorganic experiment with identical Si0-' and IS (1600 ppm Si02,0-11 IS) showed that the presence of glucose, glutamic acid or xanthan guin did not affected the polyincrisation reaction (Fig.
  167. (2005). The development of monodispersed alumino-chromate spinel nanoparticles in doped cordierite glass, studied by in situ X-ray small and wide angle scattering and chromium X-ray spectroscopy. Joumal offon-Crystalline Solids, doi
  168. (2000). The diversity of fossil microorganisms in Archaen-age rocks. doi
  169. (1998). The Dutch-Belgian Beamline at the ESRF. doi
  170. (2004). The effect of aluminium on the biodeposition of silica in hot springs water: chemical state of alurniniurn in siliceous deposits collected along the hot spring water stream of Steep Cone hot spring in Yellowstone National Park, doi
  171. (2003). The effect of cyanobacteria on Si precipitation kinetics at neutral pH: Implications for bacterial silicification in geothermal hot springs. doi
  172. (2002). The effect of Fe on Si adsorption by Bacillus subtilis cell walls: insight into non-metabolic bacterial precipitation of silicate minerals. doi
  173. (1957). The effect of selected organic compounds on the determination of silica by the molybdenum blue method. Research London,
  174. (2006). The effects of temperature, pH and sulphide on the community structure of hyperthemophilic streamers in hot springs of northern Thailand. doi
  175. (2005). The experimental silicification of Aquificales and their role in hot spring formation. doi
  176. (2005). The kinetics and mechanisms of goethite and hematite crystallisation under alkaline conditions: An in-situ time-resolved Small Angle X-ray Scattering (SAXS) study.
  177. (2008). The kinetics and mechanisms of schwertmannite transformation to goethite and hematite under alkaline conditions. American Mineralogist, doi
  178. (1961). The kinetics of precipitation from supersaturated solid solutions. doi
  179. (1980). The kinetics of silica-water reactions. doi
  180. (1964). The kinetics ofPrecipitation,
  181. (1992). The membrane-induced proton motive force influences the metal binding ability of Bacillus subtilis cell walls.
  182. (2004). The microbial role in hot spring silicification. doi
  183. (1982). The nature of water in hydrous silica.
  184. (2004). The new materials processing beamline at the SRS Daresbury, doi
  185. (1954). The polymerization of monosilicic acid.
  186. (1960). The polymerization of silicic acid obtained by the hydrothermal treatment of quartz and the solubility of amorphous silica. The Review ofPhysical Chemistry ofJapan,
  187. (1996). The relationship between physical and chemical conditions and low microbial diversity in the Blue Lagoon geothermal lake in Iceland. doi
  188. (2007). The ribosomal database project (RDP-11): introducing myRDP space and quality controlled public data. doi
  189. (1965). The role of water in opal. doi
  190. (1995). The silica balance in the world ocean: a re-estimate.
  191. (2004). The silicomolybdic acid spectrophotometric method and its application to silicate/biopolymer interaction studies. doi
  192. (1954). The solubility of amorphous silica in water. doi
  193. (1985). The Theory of Ostwald ripening. doi
  194. (1972). The thermodynamics and kinetics of the polymerisation of silicic acid in dilute aqueous solutions. Thermochimica Acta, doi
  195. (2001). The zonation and structuring of siliceous sinter around hot springs, Yellowstone National Park, and the role of thermophilic bacteria in its deposition. doi
  196. (1993). Theory and simulation of Ostwald ripening. doi
  197. (1992). Therinophilic denitrifying bkteria: a survey of hot springs
  198. (1998). Thermocrinis ruber gen. nov., sp. nov., a pink-filament-forming hyperthermophilic bacterium isolated from Yellowstone national Park. Applied and Environmental Microbiology,
  199. (1978). Thermophilic Microorganisms and Life at High Temperatures. doi
  200. (2000). Thermus igniterrae sp. nov and Thermus antranikianii sp. nov., two,, new species from Iceland. doi
  201. (1994). Thermus scotoductus, sp. nov., a pigment-producing thermophilic bacterium from hot tap water doi
  202. (1987). Thermusfiliformis sp. nov., a filamentous caldoactive bacterium. doi
  203. (1969). Therrnus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile.
  204. (2002). Time-resolved SAXS study of nucleation and growth of silica colloids. doi
  205. (2001). Trace metal chemistry of microorganisms in geothermal sinter, Taupo Volcanic Zone, New Zealand. doi
  206. (1996). Transformation kinetics of FC73.5CUINb3SiI3.5Bq ribbons to the nanocrystalline state.
  207. (2002). Transition from nucleation and growth to Ostwald ripening. doi
  208. (1989). Types of scaling occuring by geothermal utilization
  209. (1990). Universal reaction-lin-dted colloid aggregation. Physical Review A, doi
  210. (1999). User's guide to PHREEQC (version 2)-A computer program for speciation, batch-reaction, one-dimensional transport,
  211. (1993). Visualizing dispersion morphology via ultra-rapid freezing/transmission electron microscopy. doi
  212. (2004). Water content of opal-A: Implications for the origin of laminae in Geyserite and sinter. doi
  213. (2000). Weighted Neighbor Joining: A LikelihoodBased Approach to Distance-Based Phylogeny Reconstruction. Molecular Biology and Evolution, doi

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