Skip to main content
Article thumbnail
Location of Repository

Expression of Cathepsins B, D and G in Infantile Hemangioma

By Tinte eItinteang, Daria eChudakova, Jonathan eDunne, Jonathan eDunne, Paul F Davis and Swee T Tan and Swee T Tan

Abstract

Aims: The role of the renin-angiotensin system (RAS) in the biology of infantile hemangioma (IH) represents an emerging paradigm, particularly the involvement of renin, angiotensin converting enzyme and angiotensin II. This study investigated the expression of cathepsins B, D and G, enzymes that may modulate the RAS, in IH.Materials and Methods: The expression of cathepsins B, D and G was examined using immunohistochemistry, enzyme activity assays, mass spectrometry and Nanostring gene expression assay in IH samples at different phases of development.Results: Immunohistochemical staining showed the expression of cathepsins B, D and G in proliferating and involuted IH samples. This was confirmed at the transcriptional level using Nanostring gene expression assays. Mass spectrometry confirmed identification of cathepsins D and G in all three phases of IH development, whereas cathepsin B was detected in 2/2 proliferating and 1/2 involuting lesions. Enzyme activity assays demonstrated the activity of cathepsins B and D, but not G, in all phases of IH development.Conclusions: Our data demonstrated the presence of cathepsins B, D and G in IH. Their role in modulating the RAS and the biology of IH offers potential novel targets for the management of this tumor

Topics: Renin-Angiotensin System, Cathepsin, paracrine, infantile hemangioma, angiotensin converting enzyme, Surgery, RD1-811
Publisher: Frontiers Media S.A.
Year: 2015
DOI identifier: 10.3389/fsurg.2015.00026
OAI identifier: oai:doaj.org/article:508cea78b81f49ed957ee9bf22b2a293
Journal:
Download PDF:
Sorry, we are unable to provide the full text but you may find it at the following location(s):
  • https://doaj.org/toc/2296-875X (external link)
  • http://journal.frontiersin.org... (external link)
  • https://doaj.org/article/508ce... (external link)
  • Suggested articles


    To submit an update or takedown request for this paper, please submit an Update/Correction/Removal Request.