Chemical constituents of bear bile

Abstract

目的:系统研究中国传统名贵中药熊胆中的化学成分。方法:利用大孔树脂,SEPHAdEX lH-20,OdS及正相硅胶柱等色谱手段进行分离,通过多种波谱学数据分析进行单体化合物的结构鉴定。结果:从熊胆中分离得到9个单体化合物,经结构鉴定分别为4′,7-二羟基异黄酮(1),4′,7-二羟基-6-甲氧基异黄酮(2),4′,5,7-三羟基异黄酮(3),4′-甲氧基-7-羟基异黄酮(4),牛磺熊去氧胆酸(5),牛磺鹅去氧胆酸(6),熊去氧胆酸(7),鹅去氧胆酸(8),胆固醇(9)。结论:化合物1~4为首次从熊胆中分离,也是从熊科动物中首次分离得到。Objecctive: To study the chemical constituents of bear bile.Method: The compounds were isolated by repeated column HP20 macroporous adsorption resin,Sephadex LH-20,ODS and silica gel as packing materials.The structures were identified on the basis of extensive spectroscopic data analysis and by comparison of their spectral data reported.Result: Nine compounds were identified as 4′,7-dihydroxyisoflavone(1),4′,7-dihydroxy-6-methoxyisoflavone(2),4′,6,7-trihydroxyisoflavone(3),4′-methoxy-7-hydroxyisoflavone(4),tauroursodeoxycholic acid(5),taurochenodeoxycholic acid(6),ursodeoxycholic acid(7),chenodeoxycholic acid(8),cholesterol(9).Conclusion: Compounds 1-4 were separated from bear bile for the first time.厦门市科技计划项目(3502Z20083005